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               PA3438               
Key to diagram


View in GBrowse: (?) 14 putative orthologs View metabolic pathways using PseudoCyc Acknowledgements for this annotation
Gene Information
Strain Pseudomonas aeruginosa PAO1 (Tax ID: 208964) Replicon Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name folE1  Alternate Gene Names
Locus tag PA3438 (View 7 updates) GenBank Identifier (GI) 15598634 (Precomputed BLAST results at BLINK )
Genomic location 3845775 - 3846335 (+) Feature type CDS 
Transposon Mutant Available?Transposon mutant library help Yes (View in GBrowse) Pseudomonas Ortholog Group Explanation of Orthologous Groups
Product Information
Product Name GTP cyclohydrolase I precursor (Class 2  Explanation of Product Name Confidence)
Alternate Product Names
Subcellular localization
Cytoplasmic (Class 3Explanation of Localization Confidence)
Function Classification / Gene Ontology (GO)
PseudoCAP Function Class Biosynthesis of cofactors, prosthetic groups and carriers
GO Accession GO Term Evidence
Molecular Function
GO:0003934 GTP cyclohydrolase I activity IEA
Biological Process
GO:0051188 cofactor biosynthetic process ISS
GO:0006760 folic acid and derivative metabolic process RCA
GO:0042559 pteridine and derivative biosynthetic process IEA
GO:0046654 tetrahydrofolate biosynthetic process IEA
Cellular Component
COG PredictionExplanation of COG predictions
Accession Description Category
COG0302 FolE, GTP cyclohydrolase I [Coenzyme metabolism]. Coenzyme metabolism
TIGRFAM predictionExplanation of TIGRFAM predictions
Accession Name Function Significance Role Sub Role Release No.
TIGR00063 folE GTP cyclohydrolase I 7.6e-104 Biosynthesis of cofactors, prosthetic groups, and carriers Folic acid 8.0
Pathways
Pathways Explanation of Pathways Field
Name Database Xref
Folate biosynthesis PseudoCAP
Folate biosynthesis KEGG pae00790 Computationally Generated
Metabolic pathways KEGG pae01100 Computationally Generated
Reactions
EC Number 3.5.4.16
Homology
BLINK Precomputed BLAST results at BLINK
Homologs Explanation of Homologs Field 58% similar to GTP cyclohydrolase I [Escherichia coli]
77% similar to GTP cyclohydrolase I [Rattus norvegicus]
Most Similar Human Homolog
ENSP00000419045, Percent Identity: 57.78%, E-Value: 1e-58, Ensembl Release: 56.
Description: GTP cyclohydrolase 1
Structure Features
Structure Features Explanation of Structure Features Field PS00859: GTP cyclohydrolase I signature 1
PFAM predictions Explanation of PFAM predictions
Accession Domain Start Stop E-value Release No.
PF01227 GTP_cyclohydroI, GTP cyclohydrolase I. 62 154 6e-30 23.0
 
Genomic Context
Genomic context Explanation of Genomic Context Field - The adjacent gene is homologous to folX, another gene in tetrahydrobiopterin biosynthetic pathway.
Operons/Regulons
Type operon
Genes
GeneProduct
PA3437 probable short-chain dehydrogenase
folE1 GTP cyclohydrolase I precursor
folX d-erythro-7,8-dihydroneopterin triphosphate epimerase
Evidence Computationally Predicted
PubMed 18988623
Cross-Reference DOOR-13810
Microarray Expression Data
NCBI GEO Link
Sequence data
Upstream 500 BP Region AGCCTGCGAGCGATCGTCCACAACGCCTCGGACTGGGTCGCCGAGACGCCCGGCCACGAAGCCGAGGCGTTCCAGCAACTGTTCAGCGTGCACATGCTCG CGCCCTACCTGATCAACCTGCACTGCGCCGAGCTGCTGGAACGCTCGCAGCCCGCCGACATCGTCCACCTCACCGACGACGTGGCGCGCAAGGGCAGCGC CCGGCGTATCGCCTACTGCGCGAGCAAGGCCGGGCTGGACAACCTGACCCTGTCGTTCGCCGCGCGCTTCGCGCCGAGGATCAAGGTCAACGCCATTTCA CCGGCACTGGTGATGTTCAACGACGGGGACGATGCCGAATACCGCGCCCGCACCCTGGCGAAATCCGCCCTCGGCATCGAACCGGGGCCGGAAGTCATCT ACCAGAGCCTTCGCTACCTGTTGGACAACCCCTACGTCACCGGGACCACCCTGACCGTGAACGGCGGCCGCCACGTCAAGTGAGGCGCCAAGGACCCCTT

BLAST search against this genome or other genomes in database
Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence ATGAACCCAGCACTTTCCCAGCATTACCGGGAGATCCTCGTCGGTCTCGGCGAAGACCCGCAGCGCGAAGGCCTGCTCGACACCCCGAAGCGCGCGGCCA AGGCCATGCAGTACCTCTGCCACGGCTATGGCCAGACCCTGGAGGAAATCGTCAACGGCGCGCTGTTCGCCTCGGACAACGATGAAATGGTCATCGTCCG CGACATCGAGCTGTACTCCCTGTGCGAACACCACCTCCTGCCCTTCATCGGCAAGGCTCATGTGGCGTACATTCCCACCGGCAAGGTGCTCGGCCTGTCG AAAGTGGCGCGGATCGTCGACATGTTCGCCCGCCGCCTGCAGATCCAGGAGAACCTCACCCGGCAGATCGCCGAGGCGGTCCGCCAGGTGACCAGCGCCG CCGGCGTGGCGGTAGTGATCGAGGCCCAGCACATGTGCATGATGATGCGCGGGGTGGAGAAGCAGAACTCGCAGATGTTCACCTCGGTGATGCTCGGCGC GTTCCGCGACTCGAACACCACCCGCCAGGAATTTCTGCAATTGATCGGAAGGAGCAAGTGA

BLAST search against this genome or other genomes in database


Downstream 500 BP region Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.

Protein Sequence MNPALSQHYREILVGLGEDPQREGLLDTPKRAAKAMQYLCHGYGQTLEEIVNGALFASDNDEMVIVRDIELYSLCEHHLLPFIGKAHVAYIPTGKVLGLS KVARIVDMFARRLQIQENLTRQIAEAVRQVTSAAGVAVVIEAQHMCMMMRGVEKQNSQMFTSVMLGAFRDSNTTRQEFLQLIGRSK

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK
References
PubMed Cloning and sequencing of cDNA encoding rat GTP cyclohydrolase I. The first enzyme of the tetrahydrobiopterin biosynthetic pathway.
Hatakeyama K, Inoue Y, Harada T, Kagamiyama H.
J Biol Chem. 1991 Jan 15;266(2):765-9.
PMID: 1985963
Biosynthesis of tetrahydrofolate. Sequence of GTP cyclohydrolase I from Escherichia coli.
Katzenmeier G, Schmid C, Kellermann J, Lottspeich F, Bacher A.
Biol Chem Hoppe Seyler. 1991 Nov;372(11):991-7.
PMID: 1665332
 
Alternate Annotations and Cross-References
TIGR NTL03PA03438
Systomonas PA3438
RefSeq NP_252128.1
GI 15598634
KEGG PA3438
GBrowse Click here for a GBrowse View of this and other annotations.
PseudoCyc Click here to view metabolic pathways in PseudoCyc.


We thank Cystic Fibrosis Foundation Therapeutics, Inc. for critical funding support for the Pseudomonas Genome Database. When acknowledging use of the database, please cite the article by Winsor et al. (2009; PMID:18978025) and not the web site URL. Thank you!

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