Pseudomonas Genome Database - Annotation for PA3452

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PA3452

Key to diagram

View in GBrowse:

18 putative orthologs

View metabolic pathways using PseudoCyc

Acknowledgements for this annotation

Overview
Functional Data
Sequence Data
Polymorphisms
References

Gene Information
Strain	Pseudomonas aeruginosa PAO1 (Tax ID: 208964)	Replicon	Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name	mqoA	Alternate Gene Names
Locus tag	PA3452 (View 10 updates) Submit an Update	GenBank Identifier (GI)	15598648 (Precomputed BLAST results at BLINK )
Genomic location	3856749 - 3858320 (+)	Feature type	CDS
Transposon Mutant Available?	Yes (View in GBrowse)	Pseudomonas Ortholog Group	POG002188 Orthology uncertain because more than one top BLASTp hit was identified.
Product Information
Product Name	malate:quinone oxidoreductase (Class 2 )
Alternate Product Names	malate dehydrogenase
Subcellular localization	Cytoplasmic (Class 3)
Alternate Annotations and Cross-References
TIGR	NTL03PA03452
Systomonas	PA3452
RefSeq	NP_252142.1
GI	15598648
KEGG	PA3452
GBrowse	Click here for a GBrowse View of this and other annotations.
PseudoCyc	Click here to view metabolic pathways in PseudoCyc.

Function Classification / Gene Ontology (GO)

PseudoCAP Function Class

Central intermediary metabolism
Energy metabolism

GO Accession

GO Term

Evidence

Molecular Function

GO:0008924

malate dehydrogenase (acceptor) activity

IEA

Biological Process

GO:0008152	metabolic process	ISS
GO:0006091	generation of precursor metabolites and energy	ISS
GO:0006099	tricarboxylic acid cycle	IEA

Cellular Component

COG Prediction

Accession	Description	Category
COG0579	COG0579, Predicted dehydrogenase [General function prediction only].	General function prediction only

TIGRFAM prediction

Accession	Name	Function	Significance	Role	Sub Role	Release No.
TIGR01320	mal_quin_oxido	malate dehydrogenase (acceptor)	7.2e-287	Energy metabolism	TCA cycle	8.0

Pathways

Name	Database	Xref
Pyruvate metabolism	PseudoCAP
Citrate cycle (TCA cycle)	PseudoCAP
glycolysis+TCA+glyoxylate bypass	PseudoCyc	GLYCOLYSIS-TCA-GLYOX-BYPASS
TCA cycle -- aerobic respiration	PseudoCyc	TCA
glyoxylate bypass+TCA	PseudoCyc	TCA-GLYOX-BYPASS
Pyruvate metabolism	KEGG	pae00620	Computationally Generated

Reactions

EC Number

1.1.99.16

Homology

BLINK

Precomputed BLAST results at BLINK

Homologs

68% similar to L-malate dehydrogenase (acceptor) [Corynebacterium glutamicum]

Structure Features

4 strong transmembrane helices
PSORT predicted lipoprotein
Type II (lipoprotein) export signal predicted by LipoP (cleavage after residue 15)
Export across inner membrane confirmed by PhoA fusion screen

PFAM predictions

Accession	Domain	Start	Stop	E-value	Release No.
PF06039	Mqo, Malate:quinone oxidoreductase (Mqo).	24	514	0.0	23.0

Microarray Expression Data

NCBI GEO

Link

Upstream 500 BP Region

TCCGTCTCCTTTCGTCCGCCGGCCTGCCGCCCGGCCAACCCCCGCCTACACTGAAAACACCTTGGCCCGCTATCCAGGAGTCGCCGTCATGTTGGGGAAA CGTTCCACCGATCCCACCCCGGCCACCCGCTTTCGCAGCGATCGCATAAGTTCGGTGAATGGGCAGTACTTCTTCTCCACCCGCGAAGGGACCCTGGAAG GTCCCTATTTCACCCGTTTCGACGCGGAGCGGGAAATCGACGCGTATATCCGCAGGATGCGCCAATCGGCGGAAATCCACGCGCCGGGTTCCCGCTGAAA CGATACAGGCGCTGAATTTATTTCCCGGCGGCTGAAACGTTCAAGAAAGGGAAGGCAGGTATTCTCGGCGGAACGCCGAGGCAAATGTCACCGCATGTGA ACGCACTCACATCGGGGGTGAGTTCCAGTATCATTCACCCGGTGAATACCTGCCATGTCGTGCGTATCCACGCTTCTCCACGTTTTCTAGCGGTAAATCC

Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence

ATGAAAAAAATCTTATTGATGCTTTTGTGCGTCAGCGTGCTGGGTTGCTCCAAAAACAGCGTCGAATCGGAAAAACCGGTCGACGTGCTCCTGATCGGTG GCGGCATCATGAGCGCCACCCTGGGTACCTATCTGAACGAACTGGAACCAGGCTGGACCATCGAGATGGTCGAGCGCCTGGACAAGGTCGCCGAAGAAAG CTCCAACGGCTGGAACAACGCGGGCACCGGTCACTCGGCGTTCTGCGAACTGAACTACACCAGCGAGGCCGCGGACGGCTCGATGGATATCAGCAAGGCG GTGGCGATCAACGAGAACTTCGAGATCTCCAAGCAGTTCTGGGCCTACCAGGTCGACCGCAAGGTGCTGAACGATCCGAAGTCGTTCATCAACAACGTCC CGCACATGAGCTTCGTCTGGGGCGACGACAACGTCGCCTTCCTCAAGAAGCGCCACGCCGCCCTGCAGCACAGCTCGCTGTTCCGCGGCATGGAGTACTC GGAAGACCCCGAGCAGATCAAGCAGTGGGTGCCGCTGGTGATGGAAGGCCGCGAGCCGGGGCAGAAGATCGCCGCCACGCGCATGTCGATCGGCACCGAC GTCAACTTCGGCGAGATCACTCGCCAACTGGTGGGCTCGCTGTCGGCCAAGGACACCTTCAAGCTGCGCCTGCAACATGAGGTCCGCGACCTCAAGCGCA ACGACGACAACACCTGGACCGTGACCATGGCCGACCTGGCCAATGGCGACAAGGAAACCAGCGTCAAGGCCAGGTTCGTCTTCATCGGCGCCGGCGGCGG CGCGCTGAAGCTGCTGCAGATGTCCGGCATCCCCGAGGCCGAAGGCTACGCCGGCTTCCCGGTGGGCGGTTCGTTCCTCGCCACCACCAACCCGGACGTG GTCAAGCGCCACCTGGCCAAGGTCTACGGAAAGGCTTCGGTGGGTTCGCCGCCGATGTCGGTGCCGCACCTCGACACCCGCATGATCGACGGCAAGCCGG TTCTGCTGTTCGGTCCGTTCGCCACTTTCTCCACCAAGTTCCTGAAGAACGGCTCGCTGTGGGACCTGCCGGGCTCGGTGACCAGCGGCAACATCGGCCC GATGTTCAACGCCGGCATCGACAACTTCGATCTCAGCCAGTACCTGATCGGCCAGCTGATGCTCAGCCAGGACGACCGCATGGCCTCGCTGCGCGAGTAC TTCCCGGAAGCCCGCGACGAGGACTGGAAGCTGGTGCAGGCCGGCCAGCGCGTGCAGATCATCAAGAAGGACGCCGAGAAAGGCGGCGTACTGCAATTCG GCACCGAAGTGGTGACCGCAGCGGACGGCTCCGTCGCCGCCCTGCTCGGCGCCTCGCCGGGCGCCTCGACCGCCGCGCCGATCATGCTGTCGGTGCTGGA GAAGGCCTTCAAGGACAAGGTCGCTACCCCCGAGTGGCAGGCACGCCTGAAGGAAATCGTGCCGTCTTACGGGCGCAAGCTGAACAACGACATCGAGCTG ACCAACAGCACCCGTGCCTGGAGCAGCGAGCGCCTGCAACTGATCCACGTGCCGGTACAGCCGGAGGCCTGA

Downstream 500 BP region

Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.

Protein Sequence

MKKILLMLLCVSVLGCSKNSVESEKPVDVLLIGGGIMSATLGTYLNELEPGWTIEMVERLDKVAEESSNGWNNAGTGHSAFCELNYTSEAADGSMDISKA VAINENFEISKQFWAYQVDRKVLNDPKSFINNVPHMSFVWGDDNVAFLKKRHAALQHSSLFRGMEYSEDPEQIKQWVPLVMEGREPGQKIAATRMSIGTD VNFGEITRQLVGSLSAKDTFKLRLQHEVRDLKRNDDNTWTVTMADLANGDKETSVKARFVFIGAGGGALKLLQMSGIPEAEGYAGFPVGGSFLATTNPDV VKRHLAKVYGKASVGSPPMSVPHLDTRMIDGKPVLLFGPFATFSTKFLKNGSLWDLPGSVTSGNIGPMFNAGIDNFDLSQYLIGQLMLSQDDRMASLREY FPEARDEDWKLVQAGQRVQIIKKDAEKGGVLQFGTEVVTAADGSVAALLGASPGASTAAPIMLSVLEKAFKDKVATPEWQARLKEIVPSYGRKLNNDIEL TNSTRAWSSERLQLIHVPVQPEA

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK

SNPs

NOTE: This feature is currently open to beta-testing. We welcome feedback on how to improve this new resource. Please send any comments to pseudocap-mail@sfu.ca.

Showing SNPs occurring in PA3452 gene of Pseudomonas aeruginosa PAO1, Chromosome (3856749 to 3858320 bp)

Displaying the first five SNPs out of a total of 85 found in all isolate strains ( View all 85 )

Strain	Type	Mutation Description		Effect	Details	GBrowse
		DNA	Protein
Pseudomonas aeruginosa PA7	Transition	g.3856773T>C		Silent TTG(Leu) -> CTG(Leu)	SNP-433675	View
Pseudomonas aeruginosa PA7	Transition	g.3856868C>T		Silent ACC(Thr) -> ACT(Thr)	SNP-433676	View
Pseudomonas aeruginosa PA7	Transition	g.3856880T>C		Silent TAT(Tyr) -> TAC(Tyr)	SNP-433677	View
Pseudomonas aeruginosa C3719	Transition	g.3856898A>G		Silent CCA(Pro) -> CCG(Pro)	SNP-38210	View
Pseudomonas aeruginosa LESB58	Transition	g.3856898A>G		Silent CCA(Pro) -> CCG(Pro)	SNP-75165	View

Select to view SNPs in a specific strain

SNP Map

PubMed

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE.
Genome Res. 2005 Feb;15(2):321-9.
PMID: 15687295
A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map.
Itoh T, Aiba H, Baba T, Hayashi K, Inada T, Isono K, Kasai H, Kimura S, Kitakawa M, Kitagawa M, Makino K, Miki T, Mizobuchi K, Mori H, Mori T, Motomura K, Nakade S, Nakamura Y, Nashimoto H, Nishio Y, Oshima T, Saito N, Sampei G, Seki Y, Horiuchi T, et al.
DNA Res. 1996 Dec 31;3(6):379-92.
PMID: 9097040
Biochemical and genetic characterization of the membrane-associated malate dehydrogenase (acceptor) from Corynebacterium glutamicum.
Molenaar D, van der Rest ME, Petrovic S.
Eur J Biochem. 1998 Jun 1;254(2):395-403.
PMID: 9660197