Pseudomonas Genome Database - Annotation for PA3452

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PA3452

Key to diagram

View in GBrowse: (?)

18 putative orthologs

View metabolic pathways using PseudoCyc

Acknowledgements for this annotation

Gene Information

Strain

Pseudomonas aeruginosa PAO1 (Tax ID: 208964)

Replicon

Chromosome(5651 genes, 6264404 bp) [ RefSeq ]

Gene name

mqoA

Alternate Gene Names

Locus tag

PA3452 (View 10 updates)

GenBank Identifier (GI)

15598648 (Precomputed BLAST results at BLINK )

Genomic location

3856749 - 3858320 (+)

Feature type

CDS

Transposon Mutant Available?

Yes (View in GBrowse)

Pseudomonas Ortholog Group

POG002188
Orthology uncertain because more than one top BLASTp hit was identified.

Product Information

Product Name

malate:quinone oxidoreductase (Class 2

)

Alternate Product Names

malate dehydrogenase

Subcellular localization

Unknown (This protein may have multiple localization sites) (Class 3

)

Function Classification / Gene Ontology (GO)

PseudoCAP Function Class

Central intermediary metabolism
Energy metabolism

GO Accession

GO Term

Evidence

Molecular Function

GO:0008924

malate dehydrogenase (acceptor) activity

IEA

Biological Process

GO:0008152	metabolic process	ISS
GO:0006091	generation of precursor metabolites and energy	ISS
GO:0006099	tricarboxylic acid cycle	IEA

Cellular Component

COG Prediction

Accession	Description	Category
COG0579	COG0579, Predicted dehydrogenase [General function prediction only].	General function prediction only

TIGRFAM prediction

Accession	Name	Function	Significance	Role	Sub Role	Release No.
TIGR01320	mal_quin_oxido	malate dehydrogenase (acceptor)	7.2e-287	Energy metabolism	TCA cycle	8.0

Pathways

Name	Database	Xref
Pyruvate metabolism	PseudoCAP
Citrate cycle (TCA cycle)	PseudoCAP
glycolysis+TCA+glyoxylate bypass	PseudoCyc	GLYCOLYSIS-TCA-GLYOX-BYPASS
TCA cycle -- aerobic respiration	PseudoCyc	TCA
glyoxylate bypass+TCA	PseudoCyc	TCA-GLYOX-BYPASS
Pyruvate metabolism	KEGG	pae00620	Computationally Generated

Reactions

EC Number

1.1.99.16

Homology

BLINK

Precomputed BLAST results at BLINK

Homologs

68% similar to L-malate dehydrogenase (acceptor) [Corynebacterium glutamicum]

Structure Features

4 strong transmembrane helices
PSORT predicted lipoprotein
Type II (lipoprotein) export signal predicted by LipoP (cleavage after residue 15)
Export across inner membrane confirmed by PhoA fusion screen

PFAM predictions

Accession	Domain	Start	Stop	E-value	Release No.
PF06039	Mqo, Malate:quinone oxidoreductase (Mqo).	24	514	0.0	23.0

Microarray Expression Data

NCBI GEO

Link

Sequence data

Upstream 500 BP Region

TCCGTCTCCTTTCGTCCGCCGGCCTGCCGCCCGGCCAACCCCCGCCTACACTGAAAACACCTTGGCCCGCTATCCAGGAGTCGCCGTCATGTTGGGGAAA CGTTCCACCGATCCCACCCCGGCCACCCGCTTTCGCAGCGATCGCATAAGTTCGGTGAATGGGCAGTACTTCTTCTCCACCCGCGAAGGGACCCTGGAAG GTCCCTATTTCACCCGTTTCGACGCGGAGCGGGAAATCGACGCGTATATCCGCAGGATGCGCCAATCGGCGGAAATCCACGCGCCGGGTTCCCGCTGAAA CGATACAGGCGCTGAATTTATTTCCCGGCGGCTGAAACGTTCAAGAAAGGGAAGGCAGGTATTCTCGGCGGAACGCCGAGGCAAATGTCACCGCATGTGA ACGCACTCACATCGGGGGTGAGTTCCAGTATCATTCACCCGGTGAATACCTGCCATGTCGTGCGTATCCACGCTTCTCCACGTTTTCTAGCGGTAAATCC

Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence

ATGAAAAAAATCTTATTGATGCTTTTGTGCGTCAGCGTGCTGGGTTGCTCCAAAAACAGCGTCGAATCGGAAAAACCGGTCGACGTGCTCCTGATCGGTG GCGGCATCATGAGCGCCACCCTGGGTACCTATCTGAACGAACTGGAACCAGGCTGGACCATCGAGATGGTCGAGCGCCTGGACAAGGTCGCCGAAGAAAG CTCCAACGGCTGGAACAACGCGGGCACCGGTCACTCGGCGTTCTGCGAACTGAACTACACCAGCGAGGCCGCGGACGGCTCGATGGATATCAGCAAGGCG GTGGCGATCAACGAGAACTTCGAGATCTCCAAGCAGTTCTGGGCCTACCAGGTCGACCGCAAGGTGCTGAACGATCCGAAGTCGTTCATCAACAACGTCC CGCACATGAGCTTCGTCTGGGGCGACGACAACGTCGCCTTCCTCAAGAAGCGCCACGCCGCCCTGCAGCACAGCTCGCTGTTCCGCGGCATGGAGTACTC GGAAGACCCCGAGCAGATCAAGCAGTGGGTGCCGCTGGTGATGGAAGGCCGCGAGCCGGGGCAGAAGATCGCCGCCACGCGCATGTCGATCGGCACCGAC GTCAACTTCGGCGAGATCACTCGCCAACTGGTGGGCTCGCTGTCGGCCAAGGACACCTTCAAGCTGCGCCTGCAACATGAGGTCCGCGACCTCAAGCGCA ACGACGACAACACCTGGACCGTGACCATGGCCGACCTGGCCAATGGCGACAAGGAAACCAGCGTCAAGGCCAGGTTCGTCTTCATCGGCGCCGGCGGCGG CGCGCTGAAGCTGCTGCAGATGTCCGGCATCCCCGAGGCCGAAGGCTACGCCGGCTTCCCGGTGGGCGGTTCGTTCCTCGCCACCACCAACCCGGACGTG GTCAAGCGCCACCTGGCCAAGGTCTACGGAAAGGCTTCGGTGGGTTCGCCGCCGATGTCGGTGCCGCACCTCGACACCCGCATGATCGACGGCAAGCCGG TTCTGCTGTTCGGTCCGTTCGCCACTTTCTCCACCAAGTTCCTGAAGAACGGCTCGCTGTGGGACCTGCCGGGCTCGGTGACCAGCGGCAACATCGGCCC GATGTTCAACGCCGGCATCGACAACTTCGATCTCAGCCAGTACCTGATCGGCCAGCTGATGCTCAGCCAGGACGACCGCATGGCCTCGCTGCGCGAGTAC TTCCCGGAAGCCCGCGACGAGGACTGGAAGCTGGTGCAGGCCGGCCAGCGCGTGCAGATCATCAAGAAGGACGCCGAGAAAGGCGGCGTACTGCAATTCG GCACCGAAGTGGTGACCGCAGCGGACGGCTCCGTCGCCGCCCTGCTCGGCGCCTCGCCGGGCGCCTCGACCGCCGCGCCGATCATGCTGTCGGTGCTGGA GAAGGCCTTCAAGGACAAGGTCGCTACCCCCGAGTGGCAGGCACGCCTGAAGGAAATCGTGCCGTCTTACGGGCGCAAGCTGAACAACGACATCGAGCTG ACCAACAGCACCCGTGCCTGGAGCAGCGAGCGCCTGCAACTGATCCACGTGCCGGTACAGCCGGAGGCCTGA

Downstream 500 BP region

Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.

Protein Sequence

MKKILLMLLCVSVLGCSKNSVESEKPVDVLLIGGGIMSATLGTYLNELEPGWTIEMVERLDKVAEESSNGWNNAGTGHSAFCELNYTSEAADGSMDISKA VAINENFEISKQFWAYQVDRKVLNDPKSFINNVPHMSFVWGDDNVAFLKKRHAALQHSSLFRGMEYSEDPEQIKQWVPLVMEGREPGQKIAATRMSIGTD VNFGEITRQLVGSLSAKDTFKLRLQHEVRDLKRNDDNTWTVTMADLANGDKETSVKARFVFIGAGGGALKLLQMSGIPEAEGYAGFPVGGSFLATTNPDV VKRHLAKVYGKASVGSPPMSVPHLDTRMIDGKPVLLFGPFATFSTKFLKNGSLWDLPGSVTSGNIGPMFNAGIDNFDLSQYLIGQLMLSQDDRMASLREY FPEARDEDWKLVQAGQRVQIIKKDAEKGGVLQFGTEVVTAADGSVAALLGASPGASTAAPIMLSVLEKAFKDKVATPEWQARLKEIVPSYGRKLNNDIEL TNSTRAWSSERLQLIHVPVQPEA

References

PubMed

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE.
Genome Res. 2005 Feb;15(2):321-9.
PMID: 15687295
A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map.
Itoh T, Aiba H, Baba T, Hayashi K, Inada T, Isono K, Kasai H, Kimura S, Kitakawa M, Kitagawa M, Makino K, Miki T, Mizobuchi K, Mori H, Mori T, Motomura K, Nakade S, Nakamura Y, Nashimoto H, Nishio Y, Oshima T, Saito N, Sampei G, Seki Y, Horiuchi T, et al.
DNA Res. 1996 Dec 31;3(6):379-92.
PMID: 9097040
Biochemical and genetic characterization of the membrane-associated malate dehydrogenase (acceptor) from Corynebacterium glutamicum.
Molenaar D, van der Rest ME, Petrovic S.
Eur J Biochem. 1998 Jun 1;254(2):395-403.
PMID: 9660197

Alternate Annotations and Cross-References

TIGR

NTL03PA03452

Systomonas

PA3452

RefSeq

NP_252142.1

15598648

KEGG

PA3452

GBrowse

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PseudoCyc

Click here to view metabolic pathways in PseudoCyc.