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               PA4224               
Key to diagram


View in GBrowse: 5 putative orthologs View metabolic pathways using PseudoCyc Acknowledgements for this annotation
Gene Information
Strain Pseudomonas aeruginosa PAO1 (Tax ID: 208964) Replicon Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name pchG  Alternate Gene Names
Locus tag PA4224 (View 6 updates) Submit an Update GenBank Identifier (GI) 15599420 (Precomputed BLAST results at BLINK )
Genomic location 4731372 - 4730323 (-) Feature type CDS 
Transposon Mutant Available?Transposon mutant library help Yes (View in GBrowse) Pseudomonas Ortholog Group Explanation of Orthologous Groups
Product Information
Product Name pyochelin biosynthetic protein PchG (Class 1  Explanation of Product Name Confidence)
Alternate Product Names
Subcellular localization
Cytoplasmic (Class 3Explanation of Localization Confidence)
Alternate Annotations and Cross-References
TIGR NTL03PA04225
Systomonas PA4224
RefSeq NP_252914.1
GI 15599420
KEGG PA4224
GBrowse Click here for a GBrowse View of this and other annotations.
PseudoCyc Click here to view metabolic pathways in PseudoCyc.
Operons/Regulons
Type operon
Genes
GeneProduct
PA4222 probable ATP-binding component of ABC transporter
PA4223 probable ATP-binding component of ABC transporter
pchG pyochelin biosynthetic protein PchG
pchF pyochelin synthetase
pchE dihydroaeruginoic acid synthetase
Evidence Computationally Predicted
PubMed 18988623
Cross-Reference DOOR-13966
Genomic Context
Genomic context Explanation of Genomic Context Field - Adjacent to genes pchEF encoding pyochelin biosynthetic proteins
Function Classification / Gene Ontology (GO)
PseudoCAP Function Class Transport of small molecules
Membrane proteins
GO Accession GO Term Evidence
Molecular Function
Biological Process
GO:0006810 transport IDA
Cellular Component
GO:0016020 membrane IDA
COG PredictionExplanation of COG predictions
Accession Description Category
COG4693 PchG, Oxidoreductase (NAD-binding), involved in siderophore biosynthesis [Secondary metabolites biosynthesis, transport, and catabolism]. Secondary metabolites biosynthesis, transport, and catabolism
TIGRFAM predictionExplanation of TIGRFAM predictions
Accession Name Function Significance Role Sub Role Release No.
TIGR01761 thiaz-red thiazolinyl imide reductase 2.3e-211 No Data No Data 8.0
Virulence Factor
Name Category Source
Pyochelin Iron uptake Virulence Factor Database
Pathways
Pathways Explanation of Pathways Field
Name Database Xref
Biosynthesis of siderophore group nonribosomal peptides KEGG pae01053 Computationally Generated
Homology
BLINK Precomputed BLAST results at BLINK
Homologs Explanation of Homologs Field 99% similar to PchG [Pseudomonas aeruginosa]
Structure Features
Structure Features Explanation of Structure Features Field Two predicted transmembrane domains
PFAM predictions Explanation of PFAM predictions
Accession Domain Start Stop E-value Release No.
PF01408 GFO_IDH_MocA, Oxidoreductase family, NAD-binding Rossmann fold. 4 118 2e-15 23.0
 
Microarray Expression Data
NCBI GEO Link
Upstream 500 BP Region GACGAGCGCCTGCTGTTGTTCAGCTTCGCCGCGACCCTCGGCCTGGATACCGCGGCGCTCGGCTTCCCCGCGCCGGAACGTCTCGGCCAGGCGGTGCAGG CGGCGCTCGCGCAGACACCGGAGCGCCTGGTCGCCGAGGCGCTGGCGGGGCTGCCGGGCCTGGCCGATCTCGTCGCCCTGCGCGGCCGCGTGCTACAGGC GGCCAGCGGTAGCGCCGACGCCGTCAGCGTCGAACGCGACACCCTCTACCGGCTGTTCTGTCACTCGGTGCGTGCCAGCCAGGCCGAGGCGCCGGAGCCC TACGTCGGCGCGCTGCGGCTGTTCGTGCCGGACGCCGGCAACCCATTGGTGCCGCGCTACGCCGAGGCTCTGGAGACCCAATGGCGGGCCGCCGCGCTTG GCGCGTGCGGCATCCACGAGGTGCCCGGCGGGCACTTCGACTGCCTGGGCGAAGCCCTGGCGCAATCCTTGTCGAAACCCATGCCAGAGGAGGCGAGCCG

BLAST search against this genome or other genomes in database
Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence ATGAGCGACGTCCGTTCCGTGGTGGTCGCCGGTTCCCGGTTCGGCCAGTTCTATGCCGCCGGCGTCGCCGCCGATCCGCGCTTCGTCCTGCGCGGCATCC TTGGCCAGGGCAGCCGGCGTTCGCGGGCGTTGGCCGAACGCCTCGGGGTGGAGACCTGGTGCGAGGTCGAGGCGTTGCCCGACGATGTCCGCCTGGCCTG CGTCGCGGTCGGCGGCGCGGCACGCGGCGAGCAGGGCCCGGCGCTGGCCGAGGCGCTGATGGCGCGTGGCATCGACGTGCTGATCGAGCATCCGTTGCTG CCGCGCGAATGGCAGGACCTGCTGCGCAGCGCCGAGCGCCTGGGCCGGCGCTGCCTGCTCAACACCTTCTATCCGCAGTTGCCGGCGGTGGCGCGTTTCA TCGAGCTGGGCCGCCAGTTGCATCGCCGGCGCGGCATCCGCCACCTGGACGCGGCCTGCGGGGTGCAGGTCGGTTTCGCCACCCTGGACATCCTCGCCGC GTTGCTGGAGGGCGTTGGCCCCTGGTCGCTGGAGTCGCCCTCGAACGACCTGTCGGCGATGCGCGGGCTGTCCCTGGTGTTGGCGGAAGTGCCATTGAGC CTGCTCGTGCTCAACGAACTGGCGGCCGCCGACGACGGGCGCATGACCTTGTTGCAGCGCGTCAGCCTGACCACCGATCGCGGCACGCTGAGCCTGCTCA GCCCCCATGGCCCGTTGTTGTGGACGCCTGCGGTGGCGGTACCGGCAGAGGATGACGACGGCCTGTTCGCGCTGTTCGACGAGATAGCCGGCGAACCGCT GCCCAGCGCCCAGCTCTGGTATGCCGAACCGTGCAGCTGGGCCCAGGTGCACCAGCGCCTGTGGCCGGCGGCGGCGGCCGAGGCGCTGGCGCTGCTCGCC GACGGCGACGAGGTGCGCCGGCGCAACCAGCGCAGCCTGGAGGTCGCCGCCCTGTGGCAGCGGATCGGCGAGCGCCTTGGCTTCCCCGAGGCGCCGCCGG CGTCGCTGGCGCCGGCGAGCCTGGAACAGGTGCTGGAGCAAGCCTCGTGA

BLAST search against this genome or other genomes in database

Downstream 500 BP region Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.
Protein Sequence MSDVRSVVVAGSRFGQFYAAGVAADPRFVLRGILGQGSRRSRALAERLGVETWCEVEALPDDVRLACVAVGGAARGEQGPALAEALMARGIDVLIEHPLL PREWQDLLRSAERLGRRCLLNTFYPQLPAVARFIELGRQLHRRRGIRHLDAACGVQVGFATLDILAALLEGVGPWSLESPSNDLSAMRGLSLVLAEVPLS LLVLNELAAADDGRMTLLQRVSLTTDRGTLSLLSPHGPLLWTPAVAVPAEDDDGLFALFDEIAGEPLPSAQLWYAEPCSWAQVHQRLWPAAAAEALALLA DGDEVRRRNQRSLEVAALWQRIGERLGFPEAPPASLAPASLEQVLEQAS

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK
SNPs NOTE: This feature is currently open to beta-testing. We welcome feedback on how to improve this new resource. Please send any comments to pseudocap-mail@sfu.ca.

Showing SNPs occurring in PA4224 gene of Pseudomonas aeruginosa PAO1, Chromosome (4730323 to 4731372 bp)


Displaying the first five SNPs out of a total of 80 found in all isolate strains ( View all 80 )
Strain Type Mutation Description Effect Details GBrowse
DNA Protein
Pseudomonas aeruginosa PA14 Transversion g.4730332T>G p.Gln347His
 Missense
CAA(Gln) -> CAC(His) 		SNP-161358 View
Pseudomonas aeruginosa PA7 Transition g.4730371C>T
Silent
GCG(Ala) -> GCA(Ala) 		SNP-282187 View
Pseudomonas aeruginosa 2192 Transversion g.4730372G>T
Not determined
GCG(Ala) -> AGT() 		SNP-50867 View
Pseudomonas aeruginosa PA7 Transition g.4730384T>C p.Glu330Gly
 Missense
GAG(Glu) -> GGG(Gly) 		SNP-282188 View
Pseudomonas aeruginosa C3719 Transition g.4730386G>A
Silent
CCC(Pro) -> CCT(Pro) 		SNP-15354 View
Select to view SNPs in a specific strain
SNP Map
PubMed Essential PchG-dependent reduction in pyochelin biosynthesis of Pseudomonas aeruginosa.
Reimmann C, Patel HM, Serino L, Barone M, Walsh CT, Haas D.
J Bacteriol. 2001 Feb;183(3):813-20.
PMID: 11208777
 


We thank Cystic Fibrosis Foundation Therapeutics, Inc. for critical funding support for the Pseudomonas Genome Database. When acknowledging use of the database, please cite the article by Winsor et al. (2009; PMID:18978025) and not the web site URL. Thank you!

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