Pseudomonas Genome Database - Annotation for PA4225

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PA4225

Key to diagram

View in GBrowse:

4 putative orthologs

View metabolic pathways using PseudoCyc

Acknowledgements for this annotation

Overview
Functional Data
Sequence Data
Polymorphisms
Operons/Regulons
References

Gene Information
Strain	Pseudomonas aeruginosa PAO1 (Tax ID: 208964)	Replicon	Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name	pchF	Alternate Gene Names
Locus tag	PA4225 (View 5 updates) Submit an Update	GenBank Identifier (GI)	15599421 (Precomputed BLAST results at BLINK )
Genomic location	4736798 - 4731369 (-)	Feature type	CDS
Transposon Mutant Available?	Yes (View in GBrowse)	Pseudomonas Ortholog Group	POG001483 May contain one or more ortholog fragments (i.e. length of smallest gene in group is less than 1/4 of the median length for group)
Product Information
Product Name	pyochelin synthetase (Class 1 )
Alternate Product Names
Subcellular localization	Unknown (Class 3)
Alternate Annotations and Cross-References
TIGR	NTL03PA04226
Systomonas	PA4225
RefSeq	NP_252915.1
GI	15599421
KEGG	PA4225
GBrowse	Click here for a GBrowse View of this and other annotations.
PseudoCyc	Click here to view metabolic pathways in PseudoCyc.

Function Classification / Gene Ontology (GO)

PseudoCAP Function Class

Secreted Factors (toxins, enzymes, alginate)
Transport of small molecules

GO Accession

GO Term

Evidence

Molecular Function

Biological Process

GO:0006810	transport	IDA
GO:0042864	pyochelin biosynthetic process	IDA

Cellular Component

COG Prediction

Accession	Description	Category
COG1020	EntF, Non-ribosomal peptide synthetase modules and related proteins [Secondary metabolites biosynthesis, transport, and catabolism].	Secondary metabolites biosynthesis, transport, and catabolism

TIGRFAM prediction

Accession	Name	Function	Significance	Role	Sub Role	Release No.
TIGR01733	AA-adenyl-dom	amino acid adenylation domain	2.2e-148	No Data	No Data	8.0

Virulence Factor

Name	Category	Source
Pyochelin	Iron uptake	Virulence Factor Database

Pathways

Name	Database	Xref
Pyochelin synthesis	PseudoCAP
Biosynthesis of siderophore group nonribosomal peptides	KEGG	pae01053	Computationally Generated

Homology

BLINK

Precomputed BLAST results at BLINK

Homologs

99% similar to pyochelin synthetase [Pseudomonas aeruginosa]

Most Similar Human Homolog

ENSP00000301956, Percent Identity: 24.83%, E-Value: 9e-15, Ensembl Release: 56.
Description: Acyl-coenzyme A synthetase ACSM1, mitochondrial Precursor

Structure Features

PD00284: PHOSPHOPANTETHEINE BIOSYNTHESIS MULTIFUNCTIONAL ENZYME SYNTHETASE ANTIBIOTIC REPEAT LIGASE SYNTHASE TRANSFERASE

PFAM predictions

Accession	Domain	Start	Stop	E-value	Release No.
PF00668	Condensation, Condensation domain.	67	362	2e-28	23.0
PF08415	NRPS, Nonribosomal peptide synthase.	386	442	1e-14	23.0
PF00501	AMP-binding, AMP-binding enzyme.	541	938	1e-103	23.0
PF08242	Methyltransf_12, Methyltransferase domain.	1162	1267	1e-07	23.0
PF00550	PP-binding, Phosphopantetheine attachment site.	1414	1484	5e-05	23.0
PF00975	Thioesterase, Thioesterase domain.	1536	1801	5e-07	23.0

Microarray Expression Data

NCBI GEO

Link

Upstream 500 BP Region

CTGTGGCGCAGCGAGCGGATCGCCCTGGTCGAGGCGCTGGCACCGGGACTCGGGCTCGACGCCGCCGCGCTCCAGGCCGGCCTGGAGCAACGCCTGCCCC AGGCGATGCGGCCCGAACGCCTGTGGTGCCTGCCAAGCCTGCCGTTGAACGGCAATGGCAAGGTCGATCGTCGCCGCCTGGCCGAGAGCATGACCCGCGC ACTCGGCGAGTGTCGTCACGAGCCCTCGGCGGAGGAGCCGCTGGAAGCCCATGAGCAAGCGCTGGCCGAGTGCTGGGAAGCGGTTCTCAAACGCCCGGTG CGTCGTCGCGAGGCGAGCTTCTTCAGCCTCGGCGGCGACAGCCTGCTGGCGACCCGCCTGCTGGCCGGCATACGTGAGCGTTTCGGCGTACGCCTGGGCA TGGCCGACTTCTATCGCCAGCCGACCCTGGCCGGTCTTGCCCGCCACTTGCAGGTGCAGACCGTCGAAATCGAGGAAACCCAACTGGAAGAGGGCGTGCT

Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence

ATGAGCCTCGGCGAACTGCTGGAAACCTGCCGCAGCCGGCGCATCGAACTCTGGAGCGAGGCGGGCCGCCTGCGCTATCGCGCCCCCCAGGGTGCCCTCG ACGCCGGCCTCGCCGAGCGCCTGCGGGCCGAGCGCGAGGCCCTGCTGGAACACCTGGAAGGCGGCCCTGGCTGGCGCGCCGAACCCGACATGGCCCACCA GCGCTTCCCGCTGACCCCGGTGCAGGCCGCCTACGTGCTGGGCCGCCAGGCGGCCTTCGACTACGGCGGTAACGCCTGCCAGCTGTACGCCGAGTACGAC TGGCCGGCCGACACCGATCCGGCGCGCCTGGAGGCGGCCTGGAACGCCATGGTCGAGCGCCACCCGATGCTGCGCGCGGTGATCGAGGACAACGCCTGGC AGCGCGTGCTGCCCGAGGTGCCCTGGCAGCGGCTGACCGTGCATGCCTGCGCGGGGCTCGACGAGGCCGCTTTCCAGGCGCACCTGGAGCGGGTCCGCGA ACGCCTCGACCACGCCTGCGCGGCGCTCGACCAGTGGCCGGTCCTGCGCCCCGAGCTGAGTATCGGCCGGGATGCCTGCGTACTGCACTGCTCGGTGGAT TTCACCCTGGTCGACTACGCCAGCCTGCAATTGCTGCTTGGCGAATGGCGCCGCCGCTATCTCGATCCGCAATGGACGGCGGAACCGCTGGAGGCGACCT TCCGCGACTATGTCGGCGTCGAGCAGCGCCGACGCCAGTCGCCAGCCTGGCAGCGCGACCGCGACTGGTGGCTGGCGCGTCTCGACGCGCTACCGGGGCG TCCCGACCTGCCGCTGCGGGTGCAGCCGGACACCCGGTCCACGCGCTTCCGGCACTTCCACGCGCGCCTCGACGAGGCCGCCTGGCAGGCGCTCGGCGCG CGCGCCGGCGAACACGGCCTGAGCGCTGCCGGCGTGGCCTTGGCGGCCTTCGCCGAGACCATCGGTCGCTGGAGCCAGGCACCGGCGTTCTGTCTCAACC TGACGGTACTCAACCGGCCGCCGCTGCATCCGCAGCTGGCGCAGGTGCTCGGTGACTTCACCGCGCTCAGCCTGCTGGCAGTGGACAGCCGCCACGGCGA CAGTTTCGTCGAGCGTGCCCGACGCATCGGCGAGCAGATGTTCGACGACCTCGACCACCCGACCTTCAGCGGCGTCGACCTGCTGCGCGAACTGGCGCGC CGGCGTGGTCGCGGCGCCGATCTGATGCCGGTGGTGTTCACCAGTGGCATCGGCAGCGTGCAGCGCCTGCTCGGCGATGGCGAGGCGCCGCGCGCGCCAC GCTACATGATCAGCCAGACCCCGCAGGTCTGGCTGGACTGCCAGGTCACCGACCAGTTCGGCGGCCTGGAGATCGGCTGGGACGTACGCCTCGGGTTGTT CCCCGAGGGCCAGGCGGAAGCCATGTTCGACGACTTCGTCGGGCTGCTCCGGCGCCTGGCGCAGAGCCCGCGCGCCTGGACCGACGGCGATGCCACGGAA CCCGTCGAGGCGCCGCCGCAGGCGTTGCCCGGTAGTGCCCGGAGCATCGCCGCCGGTTTCGCCGAGCGTGCCCTGCTGACCCCCGACGCCACGGCGATCC ACGATGCCGCCGGCAGCTACAGCTACCGCCAGGTCGCCCAGCACGCCAGCGCCCTGCGCCGCGTCCTGGAAGCGCACGGCGCGGGCCGTGGCCGGCGGGT CGCGGTGATGCTGCCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCCGCCTATGTGCCGGTGGACATCCGCCAGCCTCCG CTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGTCGGCTGCGCCTGCGTGGCCATCGACC GGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATCTACACCTCCGGCTCCACCGGCACGCC AAAGGGCGTGATGCTCAGCCATGCGGCGGTGAGCAACACGCTGCTCGACATCAACCAGCGCTACGGCGTCGACGCCAACGACCGCGTCCTCGGCCTCGCC GAGCTGAGCTTCGACCTCTCGGTCTACGACTTCTTCGGCGCCACCGCGGCGGGGGCCCAGGTGGTCCTCCCGGACCCGGCGCGCGGCAGCGATCCATCGC ACTGGGCGGAACTGCTGGAACGCCACGCCATCACCCTGTGGAACTCGGTGCCGGCCCAAGGCCAGATGCTCATCGATTACCTGGAGAGCGAGCCGCAACG TCACCTGCCGGGACCGCGCTGCGTGCTCTGGTCCGGTGACTGGATTCCGGTCAGCCTGCCGACCCGCTGGTGGCGGCGCTGGCCGGACAGCGCGCTGTTC AGCCTGGGCGGCGCCACCGAGGCGGCGATCTGGTCGATCGAGCAGCCGATCCGCCCGCAGCACACCGAGCTGGCCAGCATCCCTTATGGCCGTGCCCTGC GCGGGCAGAGCGTGGAAGTCCTGGATGCCCGCGGGCGGCGCTGCCCGCCGGGCGTGCGCGGCGAGATCCATATCGGCGGGGTGGGCCTGGCGCTCGGCTA CGCCGGCGATCCGCAGCGCACCGCCGAACGCTTCGTCCGTCACCCCGATGGCCGTCGCCTGTATCGCACCGGCGACCTCGGCCGCTACCTGGCCGACGGC AGCATCGAGTTCCTCGGCCGCGAGGACGACCAGGTGAAGATTCGCGGCCACCGCATCGAACTGGCCGAACTGGACGCCGCGCTGTGCGCTCATCCGCAGG TCAACCTGGCGGCCACCGTGGTGCTCGGCGAGACCCACGAGCGCAGCCTGGCCAGCTTCGTCACCCTGCATGCGCCGGTGGAGGCTGGCGAGGATCCGCG TACGGCGCTCGACGCGGTGCGCCAGCGGGCGGCCCAGGCCTTGCGCCGCGACTGGGGCAGCGAGGAGGGCATCGCCGCGGCGGTGGCCGCACTCGACCGT GCCTGCCTCGCCTCGTTGGCCGCCTGGCTGGCCGGCAGCGGTCTGTTCGCCAGTGCGACGCCGCTGGACTTAGCCACCCTGTGCCAGCGCCTGGGTATCG CCGAGGCGCGCCAGCGCCTGCTGCGCCACTGGTTGCGCCAACTGGAGGAGGGCGGCTACCTGCGCGCCGAGGGCGAGGGCTGGCTGGGCTGCGCCGAGCG TCCCGCGCAGAGTCCGGAGGACGCCTGGACGGCGTTCGCCGGCTGCGCGCCGGCGGCGCTCTGGCCGGCCGAGCTGGTCGCCTACCTGCGTGACAGCGCG CAATCCCTCGGCGAGCAACTGGCCGGGCGGATCAGCCCGGCGGCGCTGATGTTCCCGCAGGGCTCGGCGCGCATCGCCGAGGCCATGTACAGCCAGGGCC TGCATGCCCAGGCGCTGCACGAGGCCATGGCCGAGGCCATCGCCGCCATCGTCGAGCGCCAGCCGCAACGGCGCTGGCGCCTGCTGGAGCTTGGCGCCGG CACCGCCGCCGCCAGCCGCACGGTGATCGCCCGGTTGGCGCCGCTGGTGCAGCGAGGGGCGGAGGTGGACTACCTGTTCACCGACGTTTCCAGCTACTTC CTCGCCGCCGCCCGCGAGCGCTTCGCCGACCAGCCGTGGGTACGCTTCGGCCGCTTCGACATGAACGGCGATCTTCTCGACCAGGGCGTGGCGCCGCACT CGGTGGATATCCTGCTCAGCTCCGGGGCCTTGAACAACGCGCTGGACACCCCGGCGCTGCTGGCCGGCCTGCGCGAGTTGCTGAGCGCCGACGCCTGGCT GGTGATCCAGGAACTGACGCGCGAGCACAACGAGATCAGCGTCAGCCAGAGCCTGATGATGGAAAACCCGCGCGACCTCCGCGACGAGCGCCGCCAACTG TTCGTCCACACCGGGCAATGGCTGGAGTGGCTGGCGGCACAGGGTGGCGACCTGGCTTGTGGGGTGGTGCCGCCGGGCAGCGCTCTCGACCTGCTTGGCT ACGATGTCCTGCTGGCTCGCTGCAAGACCGACCGCGCCCGCCTGGAGCCGGCCGAGCTGCTGGCCTTCGTCGAAGCGCGGGTGCCGCGCTACATGCTCCC GGCGCAGTTGCGCGTGCTCGAACGCCTGCCGGTCACCGGCAACGGCAAGATCGACCGCAAGGCCCTGACCGGCTTTGCCCGCCAGCCCCAGGCGGACCTT CGGCATGGCGTCGCGCAGGCACCGGCCGACGAACTGGAGAATGCGCTGCTGGCACTCTGGCGGGAGGTGCTGGACAACCCGTCGCTGGGCGTCGAGCAAG ACTTCTTCGGGGCCGGCGGCGACTCGCTGTTGATCGCCCAGTTGATCGCCCGTTTGCGCGAACGACTGGAAAGCGCCCGTCGGCATCCGTTCGATCGCCT GCTACGCTGGGCGCTCAGCCAGCCGACGCCGCGCGGCCTGGCCGAACGCCTGCGCAGCGCGCCGGAAGAGGGCCGTGGGCCAGCCCTGGCCGCGGCGCGC GGCGTCGCCCCGGCGCCGGCCGGCATGTCGCGCGCACCGCTCGCCGAGGGCGCGGTGGCGCTCGACCCGCTGGTGCGCCTGGTGCCCGGCGAGGGCGTGC CGCGGGTGCTGGTCCACGAAGGCCTCGGCACGCTACTGCCGTACCGCCCGCTGCTTCGCGCCCTGGGTGAGGGGCGGCCGTTGCTGGGGCTGGCCGTGCA TGACAGCGACGCCTACCTGGCGATCCCCGCCGAGCATCTCAACGCCTGCCTCGGCCGCCGCTACGCCGAGGCGCTCCATCGCGCCGGGCTACGCGAGGTC GACCTGCTCGGCTACTGCTCCGGCGGGCTGGTCGCCCTGGAGACCGCCAAGTCCCTGGTCCAGCGCGGGGTGCGCGTGCGCCAACTGGATATCGTCTCCA GCTACCGGATTCCCTACCGGGTGGACGACGAGCGCCTGCTGTTGTTCAGCTTCGCCGCGACCCTCGGCCTGGATACCGCGGCGCTCGGCTTCCCCGCGCC GGAACGTCTCGGCCAGGCGGTGCAGGCGGCGCTCGCGCAGACACCGGAGCGCCTGGTCGCCGAGGCGCTGGCGGGGCTGCCGGGCCTGGCCGATCTCGTC GCCCTGCGCGGCCGCGTGCTACAGGCGGCCAGCGGTAGCGCCGACGCCGTCAGCGTCGAACGCGACACCCTCTACCGGCTGTTCTGTCACTCGGTGCGTG CCAGCCAGGCCGAGGCGCCGGAGCCCTACGTCGGCGCGCTGCGGCTGTTCGTGCCGGACGCCGGCAACCCATTGGTGCCGCGCTACGCCGAGGCTCTGGA GACCCAATGGCGGGCCGCCGCGCTTGGCGCGTGCGGCATCCACGAGGTGCCCGGCGGGCACTTCGACTGCCTGGGCGAAGCCCTGGCGCAATCCTTGTCG AAACCCATGCCAGAGGAGGCGAGCCGATGA

Downstream 500 BP region

Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.

Protein Sequence

MSLGELLETCRSRRIELWSEAGRLRYRAPQGALDAGLAERLRAEREALLEHLEGGPGWRAEPDMAHQRFPLTPVQAAYVLGRQAAFDYGGNACQLYAEYD WPADTDPARLEAAWNAMVERHPMLRAVIEDNAWQRVLPEVPWQRLTVHACAGLDEAAFQAHLERVRERLDHACAALDQWPVLRPELSIGRDACVLHCSVD FTLVDYASLQLLLGEWRRRYLDPQWTAEPLEATFRDYVGVEQRRRQSPAWQRDRDWWLARLDALPGRPDLPLRVQPDTRSTRFRHFHARLDEAAWQALGA RAGEHGLSAAGVALAAFAETIGRWSQAPAFCLNLTVLNRPPLHPQLAQVLGDFTALSLLAVDSRHGDSFVERARRIGEQMFDDLDHPTFSGVDLLRELAR RRGRGADLMPVVFTSGIGSVQRLLGDGEAPRAPRYMISQTPQVWLDCQVTDQFGGLEIGWDVRLGLFPEGQAEAMFDDFVGLLRRLAQSPRAWTDGDATE PVEAPPQALPGSARSIAAGFAERALLTPDATAIHDAAGSYSYRQVAQHASALRRVLEAHGAGRGRRVAVMLPKSAAQLVAVIGILQAGAAYVPVDIRQPP LRRQAILASAEVVALVCLESDVPDVGCACVAIDRLAADSAWPPPPAAEVAADDLAYVIYTSGSTGTPKGVMLSHAAVSNTLLDINQRYGVDANDRVLGLA ELSFDLSVYDFFGATAAGAQVVLPDPARGSDPSHWAELLERHAITLWNSVPAQGQMLIDYLESEPQRHLPGPRCVLWSGDWIPVSLPTRWWRRWPDSALF SLGGATEAAIWSIEQPIRPQHTELASIPYGRALRGQSVEVLDARGRRCPPGVRGEIHIGGVGLALGYAGDPQRTAERFVRHPDGRRLYRTGDLGRYLADG SIEFLGREDDQVKIRGHRIELAELDAALCAHPQVNLAATVVLGETHERSLASFVTLHAPVEAGEDPRTALDAVRQRAAQALRRDWGSEEGIAAAVAALDR ACLASLAAWLAGSGLFASATPLDLATLCQRLGIAEARQRLLRHWLRQLEEGGYLRAEGEGWLGCAERPAQSPEDAWTAFAGCAPAALWPAELVAYLRDSA QSLGEQLAGRISPAALMFPQGSARIAEAMYSQGLHAQALHEAMAEAIAAIVERQPQRRWRLLELGAGTAAASRTVIARLAPLVQRGAEVDYLFTDVSSYF LAAARERFADQPWVRFGRFDMNGDLLDQGVAPHSVDILLSSGALNNALDTPALLAGLRELLSADAWLVIQELTREHNEISVSQSLMMENPRDLRDERRQL FVHTGQWLEWLAAQGGDLACGVVPPGSALDLLGYDVLLARCKTDRARLEPAELLAFVEARVPRYMLPAQLRVLERLPVTGNGKIDRKALTGFARQPQADL RHGVAQAPADELENALLALWREVLDNPSLGVEQDFFGAGGDSLLIAQLIARLRERLESARRHPFDRLLRWALSQPTPRGLAERLRSAPEEGRGPALAAAR GVAPAPAGMSRAPLAEGAVALDPLVRLVPGEGVPRVLVHEGLGTLLPYRPLLRALGEGRPLLGLAVHDSDAYLAIPAEHLNACLGRRYAEALHRAGLREV DLLGYCSGGLVALETAKSLVQRGVRVRQLDIVSSYRIPYRVDDERLLLFSFAATLGLDTAALGFPAPERLGQAVQAALAQTPERLVAEALAGLPGLADLV ALRGRVLQAASGSADAVSVERDTLYRLFCHSVRASQAEAPEPYVGALRLFVPDAGNPLVPRYAEALETQWRAAALGACGIHEVPGGHFDCLGEALAQSLS KPMPEEASR

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK

SNPs

NOTE: This feature is currently open to beta-testing. We welcome feedback on how to improve this new resource. Please send any comments to pseudocap-mail@sfu.ca.

Showing SNPs occurring in PA4225 gene of Pseudomonas aeruginosa PAO1, Chromosome (4731369 to 4736798 bp)

Displaying the first five SNPs out of a total of 417 found in all isolate strains ( View all 417 )

Strain	Type	Mutation Description		Effect	Details	GBrowse
		DNA	Protein
Pseudomonas aeruginosa PA14	Transition	g.4731373C>T	p.Arg1809Gln	Missense CGA(Arg) -> CAA(Gln)	SNP-161367	View
Pseudomonas aeruginosa PA7	Transition	g.4731375G>A		Silent AGC(Ser) -> AGT(Ser)	SNP-282222	View
Pseudomonas aeruginosa C3719	Transition	g.4731386C>T		Not determined GAG(Glu) -> AAA()	SNP-15363	View
Pseudomonas aeruginosa PA7	Transversion	g.4731398T>G	p.Lys1801Gln	Missense AAA(Lys) -> CAA(Gln)	SNP-282223	View
Pseudomonas aeruginosa PA7	Transversion	g.4731399C>G		Silent TCG(Ser) -> TCC(Ser)	SNP-282224	View

Select to view SNPs in a specific strain

SNP Map

Operons/Regulons

Type

operon

Genes

Gene	Product
PA4222	probable ATP-binding component of ABC transporter
PA4223	probable ATP-binding component of ABC transporter
pchG	pyochelin biosynthetic protein PchG
pchF	pyochelin synthetase
pchE	dihydroaeruginoic acid synthetase

Evidence

Computationally Predicted

PubMed

18988623

Cross-Reference

DOOR-13966

Genomic Context

Genomic context

- pchABCDREF (Pseudomonas aeruginosa)

PubMed

Dihydroaeruginoic acid synthetase and pyochelin synthetase, products of the pchEF genes, are induced by extracellular pyochelin in Pseudomonas aeruginosa.
Reimmann C, Serino L, Beyeler M, Haas D.
Microbiology. 1998 Nov;144 ( Pt 11):3135-48.
PMID: 9846750