Pseudomonas Genome Database - Annotation for PA4226

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PA4226

Key to diagram

View in GBrowse:

7 putative orthologs

View metabolic pathways using PseudoCyc

Acknowledgements for this annotation

Overview
Functional Data
Sequence Data
Operons/Regulons
Polymorphisms
References

Gene Information
Strain	Pseudomonas aeruginosa PAO1 (Tax ID: 208964)	Replicon	Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name	pchE	Alternate Gene Names
Locus tag	PA4226 (View 5 updates) Submit an Update	GenBank Identifier (GI)	15599422 (Precomputed BLAST results at BLINK )
Genomic location	4741111 - 4736795 (-)	Feature type	CDS
Transposon Mutant Available?	Yes (View in GBrowse)	Pseudomonas Ortholog Group	POG001483 May contain one or more ortholog fragments (i.e. length of smallest gene in group is less than 1/4 of the median length for group)
Product Information
Product Name	dihydroaeruginoic acid synthetase (Class 1 )
Alternate Product Names
Subcellular localization	Unknown (Class 3)
Alternate Annotations and Cross-References
TIGR	NTL03PA04227
Systomonas	PA4226
RefSeq	NP_252916.1
GI	15599422
KEGG	PA4226
GBrowse	Click here for a GBrowse View of this and other annotations.
PseudoCyc	Click here to view metabolic pathways in PseudoCyc.

Operons/Regulons

Type

operon

Genes

Gene	Product
PA4222	probable ATP-binding component of ABC transporter
PA4223	probable ATP-binding component of ABC transporter
pchG	pyochelin biosynthetic protein PchG
pchF	pyochelin synthetase
pchE	dihydroaeruginoic acid synthetase

Evidence

Computationally Predicted

PubMed

18988623

Cross-Reference

DOOR-13966

Genomic Context

Genomic context

- pchABCDREF (Pseudomonas aeruginosa)

Function Classification / Gene Ontology (GO)

PseudoCAP Function Class

Secreted Factors (toxins, enzymes, alginate)
Transport of small molecules

GO Accession

GO Term

Evidence

Molecular Function

Biological Process

GO:0006810	transport	IDA
GO:0042864	pyochelin biosynthetic process	IDA

Cellular Component

COG Prediction

Accession	Description	Category
COG1020	EntF, Non-ribosomal peptide synthetase modules and related proteins [Secondary metabolites biosynthesis, transport, and catabolism].	Secondary metabolites biosynthesis, transport, and catabolism

TIGRFAM prediction

Accession	Name	Function	Significance	Role	Sub Role	Release No.
TIGR01733	AA-adenyl-dom	amino acid adenylation domain	7.9e-141	No Data	No Data	8.0

Virulence Factor

Name	Category	Source
Pyochelin	Iron uptake	Virulence Factor Database

Pathways

Name	Database	Xref
Pyochelin synthesis	PseudoCAP
Biosynthesis of siderophore group nonribosomal peptides	KEGG	pae01053	Computationally Generated

Homology

BLINK

Precomputed BLAST results at BLINK

Homologs

100% similar to dihydroaeruginoic acid synthetase, peptide synthetase [Pseudomonas aeruginosa]

Most Similar Human Homolog

ENSP00000409656, Percent Identity: 28.28%, E-Value: 3e-14, Ensembl Release: 56.
Description: Acyl-CoA synthetase family member 4

Structure Features

BL00455: Putative AMP-binding domain signature
PD00284: PHOSPHOPANTETHEINE BIOSYNTHESIS MULTIFUNCTIONAL ENZYME SYNTHETASE ANTIBIOTIC REPEAT LIGASE SYNTHASE TRANSFERASE

PFAM predictions

Accession	Domain	Start	Stop	E-value	Release No.
PF00668	Condensation, Condensation domain.	103	399	3e-37	23.0
PF08415	NRPS, Nonribosomal peptide synthase.	421	476	2e-12	23.0
PF00501	AMP-binding, AMP-binding enzyme.	581	974	1e-93	23.0
PF00550	PP-binding, Phosphopantetheine attachment site.	1357	1421	1e-09	23.0

Microarray Expression Data

NCBI GEO

Link

Upstream 500 BP Region

GGAGGTCGAGCGCATCCACGCCGCCCGCGACCTGCTGGTCGGCGCCTTGCAGGAGCCGCCGTCGCTGGACACGCTGGCCAGCCGCGTGGGCATGAACCCG CGCAAGCTGACTGCCGGCTTTCGCAAGGTGTTCGGCGCCAGCGTATTCGGCTACCTGCAGGAATACCGCCTGCGCGAGGCGCACCGGATGCTCTGCGACG AAGAGGCGAATGTCTCCACGGTGGCCTACCGGGTGGGCTACAGCCCGGCGCATTTCTCCATCGCTTTCCGCAAGCGCTACGGCATCTCCCCCAGCGAGAT CCGCTGAGTCTCCGCGACGACCCCCGACCCGGCCACGGGAGGGGCGTCGCCGCTGCGGGATGACGCTGCATCTAACGAAACAGTCCGGCTGTCTAAAGCA TTGCCGGCCAGGCCTCCCAGGCAAGTGAGATAAGGTCGCATTTGATAATCGTTGCCAGGAACTGGCAACCGACCTCAGATGCTTGCCCTAGGGAGCCCCC

Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence

ATGGATCTGCCCCCCGATTCCCGTACCGCCCTGCGCGACTGGCTGACCGAGCAGCTCGCCGACCTGCTCGGCGAACCGCTTGCTGACGTGCGCGCCCTGG CGGACGACGACGACCTGCTGGGCTGCGGCCTCGACTCGATCCGCCTGATGTACCTGCAGGAACGCCTGCGCGCGCGTGGCTCGACGCTGGACTTCGCCCA GTTGGCGCAGCGCCCCTGCCTGGGGGCCTGGCTCGACCTGCTGGCCTGCGCGGACCGGCTGTCCGCCCCGGCAACGGTCGCGCTGCCGACGGCGCAGGAT CGCGATCAGCCGTTCGAGCTGTCTTCCGTGCAGCAGGCCTACTGGCTGGGACGTGGCGCCGGCGAGGTGCTGGGCAACGTCAGCTGCCATGCCTTTCTGG AATTCCGCACGCGGGATGTCGACCCGCAGCGCCTGGCCGCGGCGGCGGAGTGCGTGCGTCAACGCCACCCGATGTTGCGGGCGCGCTTCCTCGACGGTCG CCAGCAGATCCTTCCGACGCCGCCGCTGTCCTGCTTCGACCTGCAGGACTGGCGCACCTTACAGGTGGACGAGGCCGAGCGCGACTGGCAGGCGCTGCGC GACTGGCGCGCCCATGAATGCCTGGCGGTGGAGCGCGGCCAGGTGTTCCTGCTCGGGCTGGTGCGCATGCCGGGCGGCGAGGATCGCCTCTGGCTGAGTC TCGACCTGCTTGCCGCCGATGTCGAAAGCCTGCGCCTGCTGCTGGCCGAACTGGGCGTTGCCTACCTGGCGCCGGAGCGCCTGGCGGAGCCGCCGGCGCT GCATTTCGCCGACTACCTGGCGCACCGTGCGGCGCAACGCGCCGAGGCCGCGGCGCGGGCCCGCGACTACTGGCTGGAACGCCTGCCGCGCTTGCCGGAC GCGCCGGCCCTGCCGTTGGCCTGCGCGCCGGAAAGCATCCGCCAGCCGCGCACCCGGCGCCTGGCATTCCAGCTTTCCGCCGGCGAGAGCCGGCGCCTGG AGCGTCTTGCCGCGCAGCATGGCGTGACCTTGTCCAGCGTGTTCGGCTGCGCCTTCGCGCTGGTCCTGGCGCGCTGGAGCGAAAGCGCGGAATTTCTCCT CAACGTGCCGTTGTTCGATCGGCATGCCGACGACCCGCGTATCGGCGAGGTGATCGCCGACTTCACCACCCTGTTGCTGCTGGAGTGCCGGATGCAGGCC GGGGTGTCCTTCGCCGAGGCGGTGAAGAGCTTCCAGCGCAACCTCCACGGAGCCATCGACCACGCCGCATTCCCCGCCCTGGAGGTGCTCCGCGAGGCGC GCCGGCAGGGCCAGCCACGCTCGGCGCCGGTGGTGTTCGCCAGCAACCTGGGCGAGGAGGGCTTCGTCCCGGCGGCCTTCCGCGACGCTTTCGGCGATCT CCACGACATGCTCTCGCAGACCCCGCAGGTCTGGCTCGACCACCAGCTCTACCGGGTGGGCGACGGTATCCTGCTGGCCTGGGATAGCGTCGTCGGCCTG TTCCCCGAAGGTCTGCCGGAAACCATGTTCGAAGCCTACGTGGGGCTGCTCCAGCGTCTCTGCGACAGCGCCTGGGGGCAGCCCGCCGATCTGCCGTTGC CCTGGGCGCAGCAGGCGCGCCGGGCCCTGCTCAACGGCCAGCCGGCATGCGCCACGGCGCGCACCCTGCATCGCGACTTCTTCCTTCGCGCCGCCGAGGC GCCGGATGCCGACGCGCTGCTCTATCGCGACCAACGTGTCACCCGCGGCGAACTGGCCGAGCGTGCGCTGCGCATCGCCGGCGGCCTGCGCGAAGCCGGG GTGCGCCCTGGCGACGCGGTCGAGGTCAGCCTGCCGCGCGGACCGCAGCAGGTCGCGGCGGTATTCGGCGTGCTCGCCGCAGGCGCCTGCTACGTGCCGC TGGACATCGACCAGCCGCCCGCACGGCGGCGCCTGATCGAAGAGGCCGCCGGGGTATGCCTGGCGATCACCGAGGAGGACGATCCGCAGGCCTTGCCGCC GCGCCTGGATGTCCAGCGCCTGCTGCGCGGCCCGGCGCTGGCCGCCCCCGTGCCGCTGGCGCCGCAGGCGAGTGCCTATGTGATCTACACCTCGGGCTCC ACCGGGGTGCCCAAGGGCGTCGAGGTCAGCCACGCGGCGGCGATCAATACCATCGACGCGCTGCTCGACCTGCTGCGGGTGAACGCATCGGATCGCTTGC TGGCGGTCTCCGCGCTGGACTTCGATCTGTCGGTCTTCGACCTGTTCGGCGGCCTCGGCGCCGGTGCCAGCCTGGTCCTGCCGGCCCAGGAACAGGCGCG CGATGCCGCTGCCTGGGCGGAGGCTATCCAGCGGCATGCGGTGAGCCTGTGGAACTCGGCGCCGGCCTTGCTGGAGATGGCCCTCAGCCTGCCGGCGAGC CAGGCCGACTATCGCAGTCTGCGGGCGGTGCTGCTGTCCGGCGACTGGGTGGCCCTGGACCTGCCCGGCCGCCTGCGCCCACGTTGTGCCGAAGGCTGCC GCCTGCATGTGCTGGGTGGCGCTACCGAAGCGGGCATCTGGTCGAACCTGCAGAGCGTCGATACGGTGCCGCCGCACTGGCGTTCGATTCCCTACGGCCG GCCATTGCCGGGACAGGCCTACCGGGTGGTCGACACCCACGGGCGCGACGTGCCGGACCTGGTGGTCGGCGAGCTGTGGATCGGCGGCGCCAGCCTGGCC CGCGGCTATCGCAACGATCCCGAACTCAGCGCCCGGCGTTTCGTCCACGATGCCCAGGGCCGCTGGTATCGCACCGGCGATCGCGGTCGCTACTGGGGCG ACGGTACCCTGGAATTCCTCGGTCGGGTCGACCAGCAGGTGAAAGTGCGCGGCCAGCGCATCGAGTTGGGCGAGGTGGAGGCCGCGCTGTGCGCCCAGGC TGGCGTGGAGAGCGCCTGCGCGGCGGTGCTCGGCGGTGGCGTGGCGAGCCTCGGCGCGGTGCTGGTACCGCGCCTGGCGCCACGGGCCGAAGGCTCCATG GATCTACCGGCCGCACAGCCCTTCGCCGGCCTGGCAGAGGCCGAGGCGGTACTCACCCGGGAAATCCTCGGCGCGCTGCTGGAGGCGCCGCTGGAGCTAG ACGACGGTTTGCGCCGGCGCTGGCTGGACTGGCTAGCGGACTCCGCCGCCAGCGCGCTGCCGTCGCTCGACGAGGCGTTGCGCCGGCTCGGCTGGCAGGC CGCGGGGCTGACCGCGATGGGCAACGCTCTGCGCGGCCTGCTCGCCGGCGAACAGGCGCCGGCCGCGCTGCTCCTCGATCCCTGGCTGGCGCCGCAGGCG GTGGCCGCGCGCCTGCCGGACGGCCGCGAGGCCCTGGCGCGCCTGCTCGAAGCGCTGCCGACGCCGGCTGCCGGCGAACGCCTGCGGGTGGCGGTGCTGG ATACCCGCGCCGGGCTCTGGCTCGACCAGGGCATGGCCTCGCTGTTGCGCCCAGGGCTGGAACTGACCCTCTTCGAACGCAGCCGCGTCCTCCTCGACGC CGCCGCCACCCGCTTGCCGGAACGGATCGTGGTGCAGGCGCTGGACGACGGCCTGCTACCTGCCGAGCACCTCGGTCGCTACGACCGGGTGATCAGCTTC GCCGCGCTGCACGCCTACGAGGCCAGCCGCGAAGGCCTGGCGCTGGCGGCGGCGCTGCTGCGCCCGCAGGGCCGCCTGTTGCTGGTGGACCTGCTATGCG AGTCGCCACTGGCGCTGCTCGGTGCGGCCTTGCTCGACGACCGGCCGCTGCGCCTGGCGGAGCTGCCGAGCCTGTTGGCCGATCTCGCCGCTGCGGGACT GGCGCCGCGTTGCCTGTGGCGCAGCGAGCGGATCGCCCTGGTCGAGGCGCTGGCACCGGGACTCGGGCTCGACGCCGCCGCGCTCCAGGCCGGCCTGGAG CAACGCCTGCCCCAGGCGATGCGGCCCGAACGCCTGTGGTGCCTGCCAAGCCTGCCGTTGAACGGCAATGGCAAGGTCGATCGTCGCCGCCTGGCCGAGA GCATGACCCGCGCACTCGGCGAGTGTCGTCACGAGCCCTCGGCGGAGGAGCCGCTGGAAGCCCATGAGCAAGCGCTGGCCGAGTGCTGGGAAGCGGTTCT CAAACGCCCGGTGCGTCGTCGCGAGGCGAGCTTCTTCAGCCTCGGCGGCGACAGCCTGCTGGCGACCCGCCTGCTGGCCGGCATACGTGAGCGTTTCGGC GTACGCCTGGGCATGGCCGACTTCTATCGCCAGCCGACCCTGGCCGGTCTTGCCCGCCACTTGCAGGTGCAGACCGTCGAAATCGAGGAAACCCAACTGG AAGAGGGCGTGCTATGA

Downstream 500 BP region

Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.

Protein Sequence

MDLPPDSRTALRDWLTEQLADLLGEPLADVRALADDDDLLGCGLDSIRLMYLQERLRARGSTLDFAQLAQRPCLGAWLDLLACADRLSAPATVALPTAQD RDQPFELSSVQQAYWLGRGAGEVLGNVSCHAFLEFRTRDVDPQRLAAAAECVRQRHPMLRARFLDGRQQILPTPPLSCFDLQDWRTLQVDEAERDWQALR DWRAHECLAVERGQVFLLGLVRMPGGEDRLWLSLDLLAADVESLRLLLAELGVAYLAPERLAEPPALHFADYLAHRAAQRAEAAARARDYWLERLPRLPD APALPLACAPESIRQPRTRRLAFQLSAGESRRLERLAAQHGVTLSSVFGCAFALVLARWSESAEFLLNVPLFDRHADDPRIGEVIADFTTLLLLECRMQA GVSFAEAVKSFQRNLHGAIDHAAFPALEVLREARRQGQPRSAPVVFASNLGEEGFVPAAFRDAFGDLHDMLSQTPQVWLDHQLYRVGDGILLAWDSVVGL FPEGLPETMFEAYVGLLQRLCDSAWGQPADLPLPWAQQARRALLNGQPACATARTLHRDFFLRAAEAPDADALLYRDQRVTRGELAERALRIAGGLREAG VRPGDAVEVSLPRGPQQVAAVFGVLAAGACYVPLDIDQPPARRRLIEEAAGVCLAITEEDDPQALPPRLDVQRLLRGPALAAPVPLAPQASAYVIYTSGS TGVPKGVEVSHAAAINTIDALLDLLRVNASDRLLAVSALDFDLSVFDLFGGLGAGASLVLPAQEQARDAAAWAEAIQRHAVSLWNSAPALLEMALSLPAS QADYRSLRAVLLSGDWVALDLPGRLRPRCAEGCRLHVLGGATEAGIWSNLQSVDTVPPHWRSIPYGRPLPGQAYRVVDTHGRDVPDLVVGELWIGGASLA RGYRNDPELSARRFVHDAQGRWYRTGDRGRYWGDGTLEFLGRVDQQVKVRGQRIELGEVEAALCAQAGVESACAAVLGGGVASLGAVLVPRLAPRAEGSM DLPAAQPFAGLAEAEAVLTREILGALLEAPLELDDGLRRRWLDWLADSAASALPSLDEALRRLGWQAAGLTAMGNALRGLLAGEQAPAALLLDPWLAPQA VAARLPDGREALARLLEALPTPAAGERLRVAVLDTRAGLWLDQGMASLLRPGLELTLFERSRVLLDAAATRLPERIVVQALDDGLLPAEHLGRYDRVISF AALHAYEASREGLALAAALLRPQGRLLLVDLLCESPLALLGAALLDDRPLRLAELPSLLADLAAAGLAPRCLWRSERIALVEALAPGLGLDAAALQAGLE QRLPQAMRPERLWCLPSLPLNGNGKVDRRRLAESMTRALGECRHEPSAEEPLEAHEQALAECWEAVLKRPVRRREASFFSLGGDSLLATRLLAGIRERFG VRLGMADFYRQPTLAGLARHLQVQTVEIEETQLEEGVL

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK

SNPs

NOTE: This feature is currently open to beta-testing. We welcome feedback on how to improve this new resource. Please send any comments to pseudocap-mail@sfu.ca.

Showing SNPs occurring in PA4226 gene of Pseudomonas aeruginosa PAO1, Chromosome (4736795 to 4741111 bp)

Displaying the first five SNPs out of a total of 400 found in all isolate strains ( View all 400 )

Strain	Type	Mutation Description		Effect	Details	GBrowse
		DNA	Protein
Pseudomonas aeruginosa PA14	Transition	g.4736810T>C		Silent GAA(Glu) -> GAG(Glu)	SNP-161452	View
Pseudomonas aeruginosa PA14	Transition	g.4736831T>C		Silent GAA(Glu) -> GAG(Glu)	SNP-161453	View
Pseudomonas aeruginosa PA7	Transversion	g.4736835A>T	p.Val1426Asp	Missense GTC(Val) -> GAC(Asp)	SNP-282413	View
Pseudomonas aeruginosa C3719	Transition	g.4736844A>G		Not determined GTG(Val) -> GCG()	SNP-18227	View
Pseudomonas aeruginosa 2192	Transition	g.4736844A>G		Not determined GTG(Val) -> GCG()	SNP-63039	View

Select to view SNPs in a specific strain

SNP Map

PubMed

Dihydroaeruginoic acid synthetase and pyochelin synthetase, products of the pchEF genes, are induced by extracellular pyochelin in Pseudomonas aeruginosa.
Reimmann C, Serino L, Beyeler M, Haas D.
Microbiology. 1998 Nov;144 ( Pt 11):3135-48.
PMID: 9846750