Pseudomonas Genome Database - Annotation for PA4227

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PA4227

Key to diagram

View in GBrowse:

6 putative orthologs

View metabolic pathways using PseudoCyc

Acknowledgements for this annotation

Overview
Functional Data
Sequence Data
Polymorphisms
References

Gene Information
Strain	Pseudomonas aeruginosa PAO1 (Tax ID: 208964)	Replicon	Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name	pchR	Alternate Gene Names
Locus tag	PA4227 (View 5 updates) Submit an Update	GenBank Identifier (GI)	15599423 (Precomputed BLAST results at BLINK )
Genomic location	4742195 - 4741305 (-)	Feature type	CDS
Transposon Mutant Available?	Yes (View in GBrowse)	Pseudomonas Ortholog Group	POG001482
Product Information
Product Name	transcriptional regulator PchR (Class 1 )
Alternate Product Names
Subcellular localization	Cytoplasmic (Class 1) Cytoplasmic (Class 3)
Alternate Annotations and Cross-References
TIGR	NTL03PA04228
Systomonas	PA4227
RefSeq	NP_252917.1
GI	15599423
KEGG	PA4227
GBrowse	Click here for a GBrowse View of this and other annotations.
PseudoCyc	Click here to view metabolic pathways in PseudoCyc.

Function Classification / Gene Ontology (GO)

PseudoCAP Function Class

Transcriptional regulators

GO Accession

GO Term

Evidence

Molecular Function

GO:0030528

transcription regulator activity

IDA

Biological Process

GO:0006355

regulation of transcription, DNA-dependent

IDA

Cellular Component

COG Prediction

Accession	Description	Category
COG2207	AraC, AraC-type DNA-binding domain-containing proteins [Transcription].	Transcription

Virulence Factor

Name	Category	Source
Pyochelin	Iron uptake	Virulence Factor Database

Homology

BLINK

Precomputed BLAST results at BLINK

Homologs

100% similar to regulatory protein PchR [Pseudomonas aeruginosa]

Structure Features

PS00041: Bacterial regulatory proteins, araC family signature

PFAM predictions

Accession	Domain	Start	Stop	E-value	Release No.
PF00165	HTH_AraC, Bacterial regulatory helix-turn-helix proteins, AraC family.	254	296	4e-05	23.0

Microarray Expression Data

NCBI GEO

Link

Upstream 500 BP Region

AGGTGCAGCACTACCGTGTCGCCATGTGCGAGGCCGGCCTGGCGCAGTCCGGCTGCCAGGCGGCGGCAGCGCTGGAGCAGGTCGGCATAGCTGAGCCGCT GGTCGTCGTCGCAGAGCGCGATCCGCCGCGGATGGCGCGCGGCGCTGGTGGCCAGGGCTTCGGCGAAGCTCTGGTCCTGCCAGTGGCCGGCGTCGAGATA GCGGCGGACGAAGGCGGCGGGCCAGTCGGGGGCGTCGTCGACGGCCGATGGGGTGACGGGCGAGGAAGTCATGCGATCTCCGTGGATGCGGTCGATTGCC ATTGTTGGGAAATGAGATTTATTATCATTGGCGGGCGGCAGGGACCGCGCAAGCCCGCGCCTTTCGATTGCGGGGCTTTTTCATTCGCTGCAGGGGCGGA GTGCAGGGCGCTTTGTCCATCTCCGCTAAAAAGTCCCCTGCCCCCACGTATCCAGGAAGGCGTCGCCGCGTCGGCAGCGCCCGGGCTACAGGAAAACCTG

Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence

ATGACCATCACCATCATTGCTCCGCCACAGGCCGATGCCGCCGCGCCGGCACCGGGCAACCGGCCCGGCGTCGCGCACATCGATCCGAACATGAAGCTGG TGACCGGAACCTTCTGTTCGGCCAGCGAGGACTGGTTCGAGGAACCGCTGGAGCGCGGCCTGCGCCTGATCCTCGTGCAGAGCGGCCAGTTGCGCTGCCG GATTCCCGGCCAGCCCGAGCACCTCATCGAGGGGCCGAGCCTGTGCACCATCGCCAACGATGGCGACTTCACCTCGGCGCAGATCTACGGCACCGACAAG CCGTTGCGCTACACCATCGTCCAGCTCGGCGTCGAAGCCCTGGACAGCCGCCTCGGCTGGCTGCCCGAGCAACTGATACGCCGCCCCGGCGGCGATCCGC GGATCATGAGCTGCCCGGCGCCGCGGGCGATGCAGGCGTTGGCCTCGCAGATCGCCACCTGCCAGATGCTCGGTCCGACCCGCGATCTCTACCTCGGCGG CAAGGCCCTGGAGTTGGCCGCGCTCAGCGCACAGTTCCTTTCCGGCGAGGGGCGCCCCGTCGAGGAGCCGCGCATCACCTGCTCGGAGGTCGAGCGCATC CACGCCGCCCGCGACCTGCTGGTCGGCGCCTTGCAGGAGCCGCCGTCGCTGGACACGCTGGCCAGCCGCGTGGGCATGAACCCGCGCAAGCTGACTGCCG GCTTTCGCAAGGTGTTCGGCGCCAGCGTATTCGGCTACCTGCAGGAATACCGCCTGCGCGAGGCGCACCGGATGCTCTGCGACGAAGAGGCGAATGTCTC CACGGTGGCCTACCGGGTGGGCTACAGCCCGGCGCATTTCTCCATCGCTTTCCGCAAGCGCTACGGCATCTCCCCCAGCGAGATCCGCTGA

Downstream 500 BP region

Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.

Protein Sequence

MTITIIAPPQADAAAPAPGNRPGVAHIDPNMKLVTGTFCSASEDWFEEPLERGLRLILVQSGQLRCRIPGQPEHLIEGPSLCTIANDGDFTSAQIYGTDK PLRYTIVQLGVEALDSRLGWLPEQLIRRPGGDPRIMSCPAPRAMQALASQIATCQMLGPTRDLYLGGKALELAALSAQFLSGEGRPVEEPRITCSEVERI HAARDLLVGALQEPPSLDTLASRVGMNPRKLTAGFRKVFGASVFGYLQEYRLREAHRMLCDEEANVSTVAYRVGYSPAHFSIAFRKRYGISPSEIR

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK

SNPs

NOTE: This feature is currently open to beta-testing. We welcome feedback on how to improve this new resource. Please send any comments to pseudocap-mail@sfu.ca.

Showing SNPs occurring in PA4227 gene of Pseudomonas aeruginosa PAO1, Chromosome (4741305 to 4742195 bp)

Displaying the first five SNPs out of a total of 90 found in all isolate strains ( View all 90 )

Strain	Type	Mutation Description		Effect	Details	GBrowse
		DNA	Protein
Pseudomonas aeruginosa PA7	Transition	g.4741314C>T		Silent GAG(Glu) -> GAA(Glu)	SNP-282692	View
Pseudomonas aeruginosa PA7	Transition	g.4741326G>A		Silent ATC(Ile) -> ATT(Ile)	SNP-282693	View
Pseudomonas aeruginosa PA7	Transition	g.4741347A>G		Silent GCT(Ala) -> GCC(Ala)	SNP-282694	View
Pseudomonas aeruginosa PA7	Transition	g.4741356G>A		Silent TTC(Phe) -> TTT(Phe)	SNP-282695	View
Pseudomonas aeruginosa PA7	Transition	g.4741368G>A		Silent AGC(Ser) -> AGT(Ser)	SNP-282696	View

Select to view SNPs in a specific strain

SNP Map

PubMed

PchR, a regulator of ferripyochelin receptor gene (fptA) expression in Pseudomonas aeruginosa, functions both as an activator and as a repressor.
Heinrichs DE, Poole K.
J Bacteriol. 1996 May;178(9):2586-92.
PMID: 8626326
Cloning and sequence analysis of a gene (pchR) encoding an AraC family activator of pyochelin and ferripyochelin receptor synthesis in Pseudomonas aeruginosa.
Heinrichs DE, Poole K.
J Bacteriol. 1993 Sep;175(18):5882-9.
PMID: 8397186
Biosynthesis of pyochelin and dihydroaeruginoic acid requires the iron-regulated pchDCBA operon in Pseudomonas aeruginosa.
Serino L, Reimmann C, Visca P, Beyeler M, Chiesa VD, Haas D.
J Bacteriol. 1997 Jan;179(1):248-57.
PMID: 8982005