contact us| site map | view my clipboard
Add this annotation to your clipboard
               PA4228               
Key to diagram


View in GBrowse: 7 putative orthologs View metabolic pathways using PseudoCyc Acknowledgements for this annotation
Gene Information
Strain Pseudomonas aeruginosa PAO1 (Tax ID: 208964) Replicon Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name pchD  Alternate Gene Names
Locus tag PA4228 (View 7 updates) Submit an Update GenBank Identifier (GI) 15599424 (Precomputed BLAST results at BLINK )
Genomic location 4742424 - 4744067 (+) Feature type CDS 
Transposon Mutant Available?Transposon mutant library help Yes (View in GBrowse) Pseudomonas Ortholog Group Explanation of Orthologous Groups
Product Information
Product Name pyochelin biosynthesis protein PchD (Class 1  Explanation of Product Name Confidence)
Alternate Product Names
Subcellular localization
Cytoplasmic (Class 3Explanation of Localization Confidence)
Alternate Annotations and Cross-References
TIGR NTL03PA04229
Systomonas PA4228
RefSeq NP_252918.1
GI 15599424
KEGG PA4228
GBrowse Click here for a GBrowse View of this and other annotations.
PseudoCyc Click here to view metabolic pathways in PseudoCyc.
Operons/Regulons
Type operon
Genes
GeneProduct
pchD pyochelin biosynthesis protein PchD
pchC pyochelin biosynthetic protein PchC
pchB salicylate biosynthesis protein PchB
pchA salicylate biosynthesis isochorismate synthase
Evidence Computationally Predicted
PubMed 18988623
Cross-Reference DOOR-13967
Genomic Context
Genomic context Explanation of Genomic Context Field - iron-regulated pchDCBA (Pseudomonas aeruginosa)
Function Classification / Gene Ontology (GO)
PseudoCAP Function Class Secreted Factors (toxins, enzymes, alginate)
Transport of small molecules
GO Accession GO Term Evidence
Molecular Function
GO:0008668 (2,3-dihydroxybenzoyl)adenylate synthase activity IEA
Biological Process
GO:0006810 transport IDA
GO:0019290 siderophore biosynthetic process IEA
Cellular Component
COG PredictionExplanation of COG predictions
Accession Description Category
COG1021 EntE, Peptide arylation enzymes [Secondary metabolites biosynthesis, transport, and catabolism]. Secondary metabolites biosynthesis, transport, and catabolism
TIGRFAM predictionExplanation of TIGRFAM predictions
Accession Name Function Significance Role Sub Role Release No.
TIGR02275 DHB_AMP_lig 2,3-dihydroxybenzoate-AMP ligase 2.6e-274 Transport and binding proteins Cations and iron carrying compounds 8.0
Virulence Factor
Name Category Source
Pyochelin Iron uptake Virulence Factor Database
Pathways
Pathways Explanation of Pathways Field
Name Database Xref
Pyochelin synthesis PseudoCAP
Biosynthesis of siderophore group nonribosomal peptides KEGG pae01053 Computationally Generated
Homology
BLINK Precomputed BLAST results at BLINK
Homologs Explanation of Homologs Field 68% similar to 2,3-dihydroxybenzoate-AMP ligase [Bacillus subtilis]
99% similar to adenylate-forming, activating enzymes [Pseudomonas aeruginosa]
Most Similar Human Homolog
ENSP00000300441, Percent Identity: 23.71%, E-Value: 2e-20, Ensembl Release: 56.
Description: Acyl-CoA synthetase family member 2, mitochondrial Precursor
Structure Features
Structure Features Explanation of Structure Features Field BL00455: Putative AMP-binding domain signature
PFAM predictions Explanation of PFAM predictions
Accession Domain Start Stop E-value Release No.
PF00501 AMP-binding, AMP-binding enzyme. 61 470 2e-89 23.0
 
Microarray Expression Data
NCBI GEO Link
Upstream 500 BP Region GTGAAGTCGCCATCGTTGGCGATGGTGCACAGGCTCGGCCCCTCGATGAGGTGCTCGGGCTGGCCGGGAATCCGGCAGCGCAACTGGCCGCTCTGCACGA GGATCAGGCGCAGGCCGCGCTCCAGCGGTTCCTCGAACCAGTCCTCGCTGGCCGAACAGAAGGTTCCGGTCACCAGCTTCATGTTCGGATCGATGTGCGC GACGCCGGGCCGGTTGCCCGGTGCCGGCGCGGCGGCATCGGCCTGTGGCGGAGCAATGATGGTGATGGTCATCAGGTTTTCCTGTAGCCCGGGCGCTGCC GACGCGGCGACGCCTTCCTGGATACGTGGGGGCAGGGGACTTTTTAGCGGAGATGGACAAAGCGCCCTGCACTCCGCCCCTGCAGCGAATGAAAAAGCCC CGCAATCGAAAGGCGCGGGCTTGCGCGGTCCCTGCCGCCCGCCAATGATAATAAATCTCATTTCCCAACAATGGCAATCGACCGCATCCACGGAGATCGC

BLAST search against this genome or other genomes in database
Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence ATGACTTCCTCGCCCGTCACCCCATCGGCCGTCGACGACGCCCCCGACTGGCCCGCCGCCTTCGTCCGCCGCTATCTCGACGCCGGCCACTGGCAGGACC AGAGCTTCGCCGAAGCCCTGGCCACCAGCGCCGCGCGCCATCCGCGGCGGATCGCGCTCTGCGACGACGACCAGCGGCTCAGCTATGCCGACCTGCTCCA GCGCTGCCGCCGCCTGGCAGCCGGACTGCGCCAGGCCGGCCTCGCACATGGCGACACGGTAGTGCTGCACCTGCCCAACGGCATCGCCTTCGTCGAGACC TGCTTCGCCCTGTTCCAGCTCGGCGTACGCCCGGTGCTCGCCCTCCCCGCCCATCGCCAGCATGAGATCAGCGGTTTCTGTCGCTTCGCCGAGGCCAAGG CCTACATCGGCGCCGAACGGATCGACGGCTTCGACCCACGGCCGATGGCCCGTGAGCTGCTCGCCAGCGGCGCCTGCCGGATGGCGCTGATCCACGGCGA AGCCGAGGCGCCGCTGCAAGCGCTGGCACCGCTGTACCAGGCCGACGCGCTGGAAGACTGCGCCGCCCGCGCCGAGGATATCGCCTGCTTCCAGTTGTCC GGCGGCACCACCGGCACGCCGAAGCTGATTCCCCGGCGTCATCGCGAGTATCTCTACAACGTCCGCGCCAGCGCCGAGGTCTGCGGCTTCGACGAGCACA CGGTGTACCTCACCGGCTTGCCGATGGCGCACAACTTCACTCTCTGCTGCCCCGGAGTGATCGGCACGCTGCTGGCCAGCGGCCGCGTGGTGGTCAGCCA GCGCGCCGATCCCGAGCATTGCTTCGCCCTGATCGCCCGTGAACGGGTCACCCACACCGCCCTGGTACCGCCGCTGGCGATGCTCTGGCTGGATGCCCAG GAAAGCCGCCGCGCCGATCTATCCAGCCTGCGTCTGCTCCAGGTCGGCGGCTCCAGGCTCGGCAGCAGTGCCGCGCAACGCGTCGAACCGGTGCTCGGCT GCCAGTTGCAACAGGTGCTGGGAATGGCCGAGGGGCTGATCTGCTACACCCGCCTGGACGATCCGCCCGAACGCGTGCTGCATACCCAGGGCCGGCCGCT GTCGCCGGACGACGAAGTGCGGGTGGTCGACGCCGAGGGCCGCGAGGTCGGCCCCGGCGAGGTGGGCGAACTGACCGTGCGCGGTCCCTACACCATCCGC GGCTACTACCGCCTGCCTGAACACAACGCCAAGGCCTTCAGCGCGGACGGCTTCTACCGCACCGGCGACCGGGTCAGCCGCGACAAGGACGGCTACCTGG TGGTGGAGGGCCGCGACAAGGACCAGATCAACCGCGGCGGCGAGAAGATCGCCGCGGAAGAGGTGGAGAACCTGCTGATAGCCCATCCGCAGGTGCACGA CGCCACCGTCGTGGCGATGCCCGACAGCCTGCTCGGCGAGCGCACCTGCGCCTTCGTCATCCCGCGCCAGCCGGCGCCCTCGGCGCTGAAGCTGAAGCAA TACCTGCACGCCTGCGGGCTGGCCGCGTTCAAGGTGCCGGACCGCATCGAGCTGGTCCCGGCCTTCCCCCAAACCGGCATCGGCAAGATCAGCAAGAAGG ACCTGCGCGAGCGCCTGCGCCGCGAGCTGGAGGCCCGCGCATGA

BLAST search against this genome or other genomes in database

Downstream 500 BP region Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.
Protein Sequence MTSSPVTPSAVDDAPDWPAAFVRRYLDAGHWQDQSFAEALATSAARHPRRIALCDDDQRLSYADLLQRCRRLAAGLRQAGLAHGDTVVLHLPNGIAFVET CFALFQLGVRPVLALPAHRQHEISGFCRFAEAKAYIGAERIDGFDPRPMARELLASGACRMALIHGEAEAPLQALAPLYQADALEDCAARAEDIACFQLS GGTTGTPKLIPRRHREYLYNVRASAEVCGFDEHTVYLTGLPMAHNFTLCCPGVIGTLLASGRVVVSQRADPEHCFALIARERVTHTALVPPLAMLWLDAQ ESRRADLSSLRLLQVGGSRLGSSAAQRVEPVLGCQLQQVLGMAEGLICYTRLDDPPERVLHTQGRPLSPDDEVRVVDAEGREVGPGEVGELTVRGPYTIR GYYRLPEHNAKAFSADGFYRTGDRVSRDKDGYLVVEGRDKDQINRGGEKIAAEEVENLLIAHPQVHDATVVAMPDSLLGERTCAFVIPRQPAPSALKLKQ YLHACGLAAFKVPDRIELVPAFPQTGIGKISKKDLRERLRRELEARA

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK
SNPs NOTE: This feature is currently open to beta-testing. We welcome feedback on how to improve this new resource. Please send any comments to pseudocap-mail@sfu.ca.

Showing SNPs occurring in PA4228 gene of Pseudomonas aeruginosa PAO1, Chromosome (4742424 to 4744067 bp)


Displaying the first five SNPs out of a total of 193 found in all isolate strains ( View all 193 )
Strain Type Mutation Description Effect Details GBrowse
DNA Protein
Pseudomonas aeruginosa PA14 Transition g.4742440T>C p.Val6Ala
 Missense
GTC(Val) -> GCC(Ala) 		SNP-161519 View
Pseudomonas aeruginosa PA7 Transversion g.4742442A>C p.Thr7Pro
 Missense
ACC(Thr) -> CCT(Pro) 		SNP-282774 View
Pseudomonas aeruginosa PA7 Transition g.4742444C>T p.Thr7Pro
 Missense
ACC(Thr) -> CCT(Pro) 		SNP-282775 View
Pseudomonas aeruginosa PA7 Transversion g.4742446C>G p.Pro8Arg
 Missense
CCA(Pro) -> CGA(Arg) 		SNP-282776 View
Pseudomonas aeruginosa PA7 Transversion g.4742448T>A p.Ser9Thr
 Missense
TCG(Ser) -> ACG(Thr) 		SNP-282777 View
Select to view SNPs in a specific strain
SNP Map
PubMed Structural genes for salicylate biosynthesis from chorismate in Pseudomonas aeruginosa.
Serino L, Reimmann C, Baur H, Beyeler M, Visca P, Haas D.
Mol Gen Genet. 1995 Nov 15;249(2):217-28.
PMID: 7500944
Biosynthesis of pyochelin and dihydroaeruginoic acid requires the iron-regulated pchDCBA operon in Pseudomonas aeruginosa.
Serino L, Reimmann C, Visca P, Beyeler M, Chiesa VD, Haas D.
J Bacteriol. 1997 Jan;179(1):248-57.
PMID: 8982005
 


We thank Cystic Fibrosis Foundation Therapeutics, Inc. for critical funding support for the Pseudomonas Genome Database. When acknowledging use of the database, please cite the article by Winsor et al. (2009; PMID:18978025) and not the web site URL. Thank you!

| What's New | FAQs | Cystic Fibrosis | Pseudomonas aeruginosa | Who is Involved? | Annotation Approach | Get Involved | Supplemental Data | Links to More Analyses | Site Map | Acknowledgements | Contact Us |