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               PA4640               
Key to diagram


View in GBrowse: (?) 16 putative orthologs View metabolic pathways using PseudoCyc Acknowledgements for this annotation
Gene Information
Strain Pseudomonas aeruginosa PAO1 (Tax ID: 208964) Replicon Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name mqoB  Alternate Gene Names
Locus tag PA4640 (View 9 updates) GenBank Identifier (GI) 15599836 (Precomputed BLAST results at BLINK )
Genomic location 5208464 - 5209987 (+) Feature type CDS 
Transposon Mutant Available?Transposon mutant library help Yes (View in GBrowse) Pseudomonas Ortholog Group Explanation of Orthologous Groups
Product Information
Product Name malate:quinone oxidoreductase (Class 2  Explanation of Product Name Confidence)
Alternate Product Names malate dehydrogenase
Subcellular localization
Function Classification / Gene Ontology (GO)
PseudoCAP Function Class Central intermediary metabolism
Energy metabolism
GO Accession GO Term Evidence
Molecular Function
GO:0050421 nitrite reductase (NO-forming) activity IDA
GO:0008924 malate dehydrogenase (acceptor) activity IEA
Biological Process
GO:0008152 metabolic process ISS
GO:0006091 generation of precursor metabolites and energy ISS
GO:0006099 tricarboxylic acid cycle IEA
Cellular Component
COG PredictionExplanation of COG predictions
Accession Description Category
COG0579 COG0579, Predicted dehydrogenase [General function prediction only]. General function prediction only
TIGRFAM predictionExplanation of TIGRFAM predictions
Accession Name Function Significance Role Sub Role Release No.
TIGR01320 mal_quin_oxido malate dehydrogenase (acceptor) 0 Energy metabolism TCA cycle 8.0
Pathways
Pathways Explanation of Pathways Field
Name Database Xref
Pyruvate metabolism PseudoCAP
Citrate cycle (TCA cycle) PseudoCAP
glycolysis+TCA+glyoxylate bypass PseudoCyc GLYCOLYSIS-TCA-GLYOX-BYPASS
TCA cycle -- aerobic respiration PseudoCyc TCA
glyoxylate bypass+TCA PseudoCyc TCA-GLYOX-BYPASS
Pyruvate metabolism KEGG pae00620 Computationally Generated
Reactions
EC Number 1.1.99.16
Homology
BLINK Precomputed BLAST results at BLINK
Homologs Explanation of Homologs Field 69% similar to L-malate dehydrogenase (acceptor) [Corynebacterium glutamicum]
Structure Features
Structure Features Explanation of Structure Features Field 3 predicted transmembrane helices
0 predicted transmembrane helices (TMHMM v.2.0)
At least one transmembrane helix predicted by Phobius
PFAM predictions Explanation of PFAM predictions
Accession Domain Start Stop E-value Release No.
PF06039 Mqo, Malate:quinone oxidoreductase (Mqo). 5 494 0.0 23.0
PF01266 DAO, FAD dependent oxidoreductase. 14 242 2e-09 23.0
 
Microarray Expression Data
NCBI GEO Link
Sequence data
Upstream 500 BP Region CCCATGAGGCAGACGCCCTGAAACGCCTGTAGCGAGTTTTTCGGCAAGGAATGCCGCATGACCCCGCACGATTCTGCGAAGGCATGCCCGCGACAGACGA GCCCTTCCGGCCCATGTCTCCAAAAGAGCGTGGATCGAAGGTATTTCACTACTTCACGAGAGTGTGGCGAGCAATGAAGAGTTTTGCGTCGGTAAGTGCG CCATTAGCGCTTGAAACAGCCCCCCGCAGACGCGACCGCAGCGGATACCTCATGACCAGAGCCTGACGGCCCGTTCCGTCGCGAATGTGCTTGCACGACC GGATGCCCGCATGGGCCAGCCAGGCTGCCAGATCGCCAGCCCCGCGCCCGGCATCCATAGGCGATGACGCCCGGCGTCCCGCCGTTGTTGCGAAGAATCG GACATGACGGCTCCCTCCGCCGCTGCCTGCCGGCCCGTACCGGCCTCGGCCCCGCCCCGTCTGTCGTCAATTTGGTGCTGAAGATTTTGGAGAAGCGTTA

BLAST search against this genome or other genomes in database
Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence ATGGCGCAAAACGATCACGAAACAGTCGACATGCTGCTGGTAGGCGCCGGCATCATGAGCGCGACCCTGGCGGTCCTGCTCAAGGAACTCGACCCCAACC TGAAGATGGAAGTCGTCGAACTCCAGGAGTCCGGCGCGATCGAAAGCTCCAACCCGTGGAACAACGCGGGCACCGGCCACGCCGGCCTGTGCGAGCTGAA CTACACCCCGCAGTCGGCCGACGGCAGCATCGACATCAAGAAAGCCGTGGGCATCAACACCATGTTCGAGGTGTCGAAGCAGTTCTGGTCGCACCTGGTC GCCAAGGGCACCTTCGGCTCGCCGAAGACCTTCATCAACCCGGTACCGCACCTGAGCTTCGTGCGCGGCAGCGAAGGCATCGCCTACCTGAAGAAGCGCT TCGAATCGCTGACGAAGCACCACGCTTTCGAGACCATGGTCTACTCCGAGGACAAGGCCACCCTCGCCGAGTGGATGCCGCTGATGATGCCGGGTCGTCC GGCCGACGAAGCGATCGCCGCGACCCGCGTCGAGGGCGGTACCGACGTCAACTTCGGCGCCCTGACCAACCAGCTCCTGCAACACCTGGCGCAACAGCCG GGCGCGCAGATCCGCTACAACCAGAAGGTCACCCACCTGCGCCGCGCCGATAACGGCTGGCGGGTCACCGTCAAGGACACCCGCAACGGCGGCGACCGCG AGATCCAGGCACGCTTCGTCTTCCTCGGCGCCGGCGGCGGCGCCCTGCCGCTCCTGCAACTTTCCGGCATCCCGGAAGGCAAGGGCTTCGGTGGTTTCCC GGTGAGCGGCCAGTGGCTGCGCTGCGACAACCCGGAAATCGTCAAGCAGCACCAGGCCAAGGTCTACAGCCAGGCCGAGGTCGGCTCGCCGCCGATGTCC GTGCCGCACCTGGACACCCGTGTGGTGGACGGCAAGAAATCCCTGCTGTTCGGCCCCTATGCCGGTTTCTCCACCAAGTTCCTGCGCCATGGCTCGTTCC TCGACCTGCCGCTGTCGGTACGCCCGGGCAACATCCTGCCGATGCTCTCGGTGGCCCGCGACAACATGGACCTGACCCGCTACCTGATCGGCCAGGTCAT GCAGTCGCCGGAACAGCGCCTGGAAGCCCTGCGCAAGTTCTACCCCGAAGCCAGGGCCGAGGACTGGCGCCTGGAAGTCGCCGGCCAGCGCGTGCAGATC ATCAAGAAAGATCCGAAGAAAGGCGGCATCCTGCAGTTCGGCACCGAGCTGGTGGCCGCCCACGACGGCTCCATCGCCGCCCTGCTGGGCGCCTCGCCGG GCGCCTCGGTGACCGTCTCGATCATGCTCGGCCTGATCGAGCGCTGCTTCCCGGAGCAGGCCCGCTCGCCGGAGTGGAGCGCCAAGCTGAAGGAAATCTT CCCGGCTCGCGAGAAAGAGCTGGAAAGCGACGCCGAGCTGTACCGCAGCGTCAGCAGCCGTTGCAGCGAGGTGCTGGAACTGACCGCGAAGAACGACGTG CAGGCCCCGGTCAACGCCGAGTGA

BLAST search against this genome or other genomes in database


Downstream 500 BP region Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.

Protein Sequence MAQNDHETVDMLLVGAGIMSATLAVLLKELDPNLKMEVVELQESGAIESSNPWNNAGTGHAGLCELNYTPQSADGSIDIKKAVGINTMFEVSKQFWSHLV AKGTFGSPKTFINPVPHLSFVRGSEGIAYLKKRFESLTKHHAFETMVYSEDKATLAEWMPLMMPGRPADEAIAATRVEGGTDVNFGALTNQLLQHLAQQP GAQIRYNQKVTHLRRADNGWRVTVKDTRNGGDREIQARFVFLGAGGGALPLLQLSGIPEGKGFGGFPVSGQWLRCDNPEIVKQHQAKVYSQAEVGSPPMS VPHLDTRVVDGKKSLLFGPYAGFSTKFLRHGSFLDLPLSVRPGNILPMLSVARDNMDLTRYLIGQVMQSPEQRLEALRKFYPEARAEDWRLEVAGQRVQI IKKDPKKGGILQFGTELVAAHDGSIAALLGASPGASVTVSIMLGLIERCFPEQARSPEWSAKLKEIFPAREKELESDAELYRSVSSRCSEVLELTAKNDV QAPVNAE

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK
References
PubMed Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE.
Genome Res. 2005 Feb;15(2):321-9.
PMID: 15687295
Biochemical and genetic characterization of the membrane-associated malate dehydrogenase (acceptor) from Corynebacterium glutamicum.
Molenaar D, van der Rest ME, Petrovic S.
Eur J Biochem. 1998 Jun 1;254(2):395-403.
PMID: 9660197
 
Alternate Annotations and Cross-References
TIGR NTL03PA04641
Systomonas PA4640
RefSeq NP_253330.1
GI 15599836
KEGG PA4640
GBrowse Click here for a GBrowse View of this and other annotations.
PseudoCyc Click here to view metabolic pathways in PseudoCyc.


We thank Cystic Fibrosis Foundation Therapeutics, Inc. for critical funding support for the Pseudomonas Genome Database. When acknowledging use of the database, please cite the article by Winsor et al. (2009; PMID:18978025) and not the web site URL. Thank you!

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