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               PA5084               
Key to diagram


View in GBrowse: (?) 12 putative orthologs View metabolic pathways using PseudoCyc Acknowledgements for this annotation
Gene Information
Strain Pseudomonas aeruginosa PAO1 (Tax ID: 208964) Replicon Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name   Alternate Gene Names
Locus tag PA5084 (View 10 updates) GenBank Identifier (GI) 15600277 (Precomputed BLAST results at BLINK )
Genomic location 5723517 - 5722267 (-) Feature type CDS 
Transposon Mutant Available?Transposon mutant library help Yes (View in GBrowse) Pseudomonas Ortholog Group Explanation of Orthologous Groups
Product Information
Product Name probable oxidoreductase (Class 3  Explanation of Product Name Confidence)
Alternate Product Names
Subcellular localization
Function Classification / Gene Ontology (GO)
PseudoCAP Function Class Putative enzymes
GO Accession GO Term Evidence
Molecular Function
GO:0016647 oxidoreductase activity, acting on the CH-NH group of donors, oxygen as acceptor IEA
Biological Process
Cellular Component
COG PredictionExplanation of COG predictions
Accession Description Category
COG0665 DadA, Glycine/D-amino acid oxidases (deaminating) [Amino acid transport and metabolism]. Amino acid transport and metabolism
TIGRFAM predictionExplanation of TIGRFAM predictions
Accession Name Function Significance Role Sub Role Release No.
TIGR02352 thiamin_ThiO glycine oxidase ThiO 4.8e-12 Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine 8.0
Pathways
Pathways Explanation of Pathways Field
Name Database Xref
Phenylalanine metabolism KEGG pae00360 Computationally Generated
Nitrogen metabolism KEGG pae00910 Computationally Generated
Homology
BLINK Precomputed BLAST results at BLINK
Homologs Explanation of Homologs Field 55% similar to dadA gene product of [E. coli]
Structure Features
Structure Features Explanation of Structure Features Field 1-2 predicted transmembrane domains (not signal peptide?)
COG665E: D-amino-acid dehydrogenases. Weak block hit to several oxidoreductases
type I export signal computationally predicted by LipoP v.1.0 (cleavage after residue 20)
type I export signal computationally predicted by Phobius (cleavage after residue 20)
type I export signal computationally predicted by SignalP v.3.0 Hidden Markov Models (cleavage after residue 20)
PFAM predictions Explanation of PFAM predictions
Accession Domain Start Stop E-value Release No.
PF01266 DAO, FAD dependent oxidoreductase. 4 395 4e-42 23.0
PF07992 Pyr_redox_2, Pyridine nucleotide-disulphide oxidoreductase. 4 41 9e-05 23.0
PF01593 Amino_oxidase, Flavin containing amine oxidoreductase. 14 263 8e-09 23.0
 
Operons/Regulons
Type operon
Genes
GeneProduct
PA5083 conserved hypothetical protein
PA5084 probable oxidoreductase
Evidence Computationally Predicted
PubMed 18988623
Cross-Reference DOOR-14155
Microarray Expression Data
NCBI GEO Link
Sequence data
Upstream 500 BP Region GCGAATGCAAGGTGGCCAGTTCGCAGCCGCTCTGCGGATAGCGCGCGCTCCATTCGCCGATCGCCCGCGGCAGCAGCGACAGCGCCAGGGCCGGGGTGGC GCCGATGCGCAGGCTGTGGCCCGGCTGGCGCCGCAGGTTCTGCGCCAGGCGCCGCACGGCCTGCAGGCTATCGCTGAGCTTGGCCACTTCGCGCTCCAGT TCCAGCGCTTCCGGAGTCGCCAGCAGCTTGCCGCGCACTCGCAAGAACAGCGGGAAGCCCAGTTGCTGCTCGGCGTGCTGCAGGACCTTGCTCACCGCCG GTTGGGACACGTGCAGCAACTGTGCGGCGCCGCTGACCGAGCCAGCCTGGCGGATCGCCTGGAACACTTCGATATGGCGGAGCTTCATCGGCGCGAGCCC ATAACCTGGATTTATACCCTGGCCATCTTTATTCATTGTCCTACCGCTGTCATCCGCGCCTATCGTCGAAGCCCTGGAAAACACCTGGATAACGATTGTC

BLAST search against this genome or other genomes in database
Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence ATGGCTCAGCGGGTTTGCATCATCGGCGCCGGCGTGGTCGGCCTGGCCACGGCCTACGCCCTGGTTCGCGAAGGATTCGACGTGACCCTGGTGGAAGCGC GCGAGCGGGGCGGGCTGGAAACCAGCTACGCCAATGGCGGGCAGTTGTCCTATCGCTACGTGGCGCCATTGGCCGACAGCGGCGTGCCGGCCCAGGCGCT CGGCTGGCTGCTGCGCAACGATGCGCCGTTGCGCCTGCGGCCGCGTCTTGATCCGGCGCAGTGGCGCTGGCTGCTGGGCTTCCTCGCGGCCTGCCGCGGC TCGCTGAACCGCCGCAATGCCGCGCACCTGCTGCGCCTGGCATTGCTCAGCCAGCGCACCCTGCAGGACTGGCGCGAGGAGGATGGCCTCGATGGCTTCG ACTGGCGGCGCAACGGCAAGCTGGTGGCGTTTCGCCAACCGGCCAGCTTTGCGCGGGCCCGCCAGGGCCTGGCCGATCCTTCGGCGCAATTCGTCCTGAC CGCCGCCGAGTGCCTCGCGCTGGAGCCGGCCCTGTCCGCCGCGCCCTTTGTCGGCGGCATCCACACGCCGGACGAGGAGGTCGCCGACTGCCATGCCTTC TGCGTGCAACTTGCCGAGCGCCTGCAGGCTTCCGGACATTGCCGGCAACTGCATGGCCGCAGGGTGACGAAGATCGTCGAGGGCGGTGCGGCGGTGCGTG GCGTCGAGGTCGGCGGCGATCTCATCGAGGCCGATCATGTGGTGCTGGCCGCCGGCTACCGCAGCGCCGAATTGATGCTGCCGGGCCTGCGCCTGCCGCT ATACCCGCTCAAGGGCTATAGCCTGACCCTGCCGGTCGGCGCGCAGCACCGCGCGCCGGATACCAGCATCACCGATTACGACCGCAAGATCGTCTACGCG CGGATCGGCGAGCGGCTGCGGATCGCCGCGATGGTCGACATCGTCGGCTTCGACCCCGGCCTCGAGCCCGCGCGCCTGGCACTGCTGCGCCAGCAGGCGC GGGACACGTTCCCGCAGGGCGGCGATTTCGACGCTGCCGTCGAATGGGCCGGCATGCGCCCGGCCACGCCCACCGGGGTACCGCTGGTGGGCAACGGCGG CTACCGCAACCTCTGGCTCAACCTCGGCCACGGCGCGCTCGGCTTCACCCTGGCCTGCGGCAGCGGGCGCCTGCTGGCCGAGCAGATCGGCCGCCGCCCG CCGAGCATCGATACCACTGGCCTGCTGCCGCGCGGCGCGCTGGCTGGCTGA

BLAST search against this genome or other genomes in database


Downstream 500 BP region Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.

Protein Sequence MAQRVCIIGAGVVGLATAYALVREGFDVTLVEARERGGLETSYANGGQLSYRYVAPLADSGVPAQALGWLLRNDAPLRLRPRLDPAQWRWLLGFLAACRG SLNRRNAAHLLRLALLSQRTLQDWREEDGLDGFDWRRNGKLVAFRQPASFARARQGLADPSAQFVLTAAECLALEPALSAAPFVGGIHTPDEEVADCHAF CVQLAERLQASGHCRQLHGRRVTKIVEGGAAVRGVEVGGDLIEADHVVLAAGYRSAELMLPGLRLPLYPLKGYSLTLPVGAQHRAPDTSITDYDRKIVYA RIGERLRIAAMVDIVGFDPGLEPARLALLRQQARDTFPQGGDFDAAVEWAGMRPATPTGVPLVGNGGYRNLWLNLGHGALGFTLACGSGRLLAEQIGRRP PSIDTTGLLPRGALAG

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK
References
PubMed Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE.
Genome Res. 2005 Feb;15(2):321-9.
PMID: 15687295
A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.
Oshima T, Aiba H, Baba T, Fujita K, Hayashi K, Honjo A, Ikemoto K, Inada T, Itoh T, Kajihara M, Kanai K, Kashimoto K, Kimura S, Kitagawa M, Makino K, Masuda S, Miki T, Mizobuchi K, Mori H, Motomura K, Nakamura Y, Nashimoto H, Nishio Y, Saito N, Horiuchi T
DNA Res. 1996 Jun 30;3(3):137-55.
PMID: 8905232
 
Alternate Annotations and Cross-References
TIGR NTL03PA05085
Systomonas PA5084
RefSeq NP_253771.1
GI 15600277
KEGG PA5084
GBrowse Click here for a GBrowse View of this and other annotations.
PseudoCyc Click here to view metabolic pathways in PseudoCyc.


We thank Cystic Fibrosis Foundation Therapeutics, Inc. for critical funding support for the Pseudomonas Genome Database. When acknowledging use of the database, please cite the article by Winsor et al. (2009; PMID:18978025) and not the web site URL. Thank you!

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