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               PA5111               
Key to diagram


View in GBrowse: (?) 15 putative orthologs View metabolic pathways using PseudoCyc Acknowledgements for this annotation
Gene Information
Strain Pseudomonas aeruginosa PAO1 (Tax ID: 208964) Replicon Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name gloA3  Alternate Gene Names glo1
Locus tag PA5111 (View 7 updates) GenBank Identifier (GI) 15600304 (Precomputed BLAST results at BLINK )
Genomic location 5754144 - 5753614 (-) Feature type CDS 
Transposon Mutant Available?Transposon mutant library help Yes (View in GBrowse) Pseudomonas Ortholog Group Explanation of Orthologous Groups
Product Information
Product Name lactoylglutathione lyase (Class 1  Explanation of Product Name Confidence)
Alternate Product Names aldoketomutase, methylglyoxalase, glyoxalase I
Subcellular localization
Cytoplasmic (Class 3Explanation of Localization Confidence)
Function Classification / Gene Ontology (GO)
PseudoCAP Function Class Central intermediary metabolism
GO Accession GO Term Evidence
Molecular Function
GO:0003961 O-acetylhomoserine aminocarboxypropyltransferase activity ISS
GO:0004462 lactoylglutathione lyase activity IEA
Biological Process
GO:0008152 metabolic process ISS
GO:0006090 pyruvate metabolic process ISS
GO:0005975 carbohydrate metabolic process IEA
GO:0051596 methylglyoxal catabolic process IEA
Cellular Component
COG PredictionExplanation of COG predictions
Accession Description Category
COG0346 GloA, Lactoylglutathione lyase and related lyases [Amino acid transport and metabolism]. Amino acid transport and metabolism
TIGRFAM predictionExplanation of TIGRFAM predictions
Accession Name Function Significance Role Sub Role Release No.
TIGR00068 glyox_I lactoylglutathione lyase 7.3e-76 Central intermediary metabolism | Energy metabolism Amino sugars | Other 8.0
Pathways
Pathways Explanation of Pathways Field
Name Database Xref
Pyruvate metabolism PseudoCAP
methylglyoxal degradation PseudoCyc PWY-901
Pyruvate metabolism KEGG pae00620 Computationally Generated
Reactions
EC Number 4.4.1.5
Enzyme Assays
PDF file(s) provided by Sigma-Aldrich:
 Glyoxalase I (4.4.1.5)
Homology
BLINK Precomputed BLAST results at BLINK
Homologs Explanation of Homologs Field 71% similar to glyoxalase I of [Pseudomonas putida]
Most Similar Human Homolog
ENSP00000362463, Percent Identity: 57.05%, E-Value: 2e-48, Ensembl Release: 56.
Description: Lactoylglutathione lyase
Structure Features
Structure Features Explanation of Structure Features Field PS00934: Glyoxalase I signature 1
PFAM predictions Explanation of PFAM predictions
Accession Domain Start Stop E-value Release No.
PF00903 Glyoxalase, Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily. 24 164 3e-22 23.0
 
Microarray Expression Data
NCBI GEO Link
Sequence data
Upstream 500 BP Region GCGCCACCGCGCTCGACTACGACGACCAGAAGCGCAGCTCGAAGCGCCTGGGCGCCGGCCTGCAAGGCAAGTACGCGTTCGGCAGCGATACCCAGCTGTT CGCCGAGTACGCCCACGAACGTGAGTACGAGGACGACACCCAGGACCTGACCATGTCCCTCAACAGCCTGCCGGGCAATCGCTTCACCCTCGAAGGCTAC ACCCCGCAGGACCATCTCAACCGCGTCTCACTCGGCTTCAGCCAGAAGCTGGCGCCGGAGCTGTCGCTGCGCGGCGGCTACAACTGGCGCAAGGGCGAGG ACGATACCCAGCAGAGCGTCAGCCTGGCGCTGAGCCTGGACTTCTGAAACGGCGGCCGGCGCCCGGTCGGCGCCGGTCGCGTTTCCCGTCGACGGAACGA TCGGCGCGCGCCGGCATCAAGAGAGTAGTACCCCGCTGTTACTCGCCGCCCGGTCGACCGCCATACACTGCCGATCCGTTTCGTTCGAGGGAATCGCCCG

BLAST search against this genome or other genomes in database
Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence ATGAGTTTCAACACCGAAGTACAGCCCGGCATCTGCATGGAGCCGGACGCCATCACCCAGGAATACGTGTTCAACCACACCATGTTGCGGGTCAAGGATC CGAAGCGCTCGCTCGACTTCTACTCGCGGGTGCTCGGCATGCGCCTGCTGCGTCGCCTGGATTTCGAGGAAGGCCGCTTCTCCCTGTATTTCCTCGCCAT GACCCGTGGCGAAGAAGTGCCTGATGCGGTTGACGAGCGCCAGCGATATACCTTCGGACGCCAGTCGGTCCTCGAGCTGACCCACAACTGGGGCAGCGAG AGCGACGACAGCCAGTACCACAACGGCAACCAGGACCCGCGCGGGTTCGGCCACATCTGCTTCTCGGTGCCCGACCTGGTGGCGGCCTGCGAGCGTTTCG AGACGCTCGGGGTGAACTTCGTCAAGCCGCTGGACCGAGGCATGAAGAACGTGGCCTTCATCAGCGATCCCGACGGCTACTGGGTGGAAATCGTCCAGGC CAGCCTGAACGGCGAGATGGGACGCGGCTGA

BLAST search against this genome or other genomes in database


Downstream 500 BP region Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.

Protein Sequence MSFNTEVQPGICMEPDAITQEYVFNHTMLRVKDPKRSLDFYSRVLGMRLLRRLDFEEGRFSLYFLAMTRGEEVPDAVDERQRYTFGRQSVLELTHNWGSE SDDSQYHNGNQDPRGFGHICFSVPDLVAACERFETLGVNFVKPLDRGMKNVAFISDPDGYWVEIVQASLNGEMGRG

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK
References
PubMed The gene encoding glyoxalase I from Pseudomonas putida: cloning, overexpression, and sequence comparisons with human glyoxalase I.
Lu T, Creighton DJ, Antoine M, Fenselau C, Lovett PS.
Gene. 1994 Dec 2;150(1):93-6.
PMID: 7959071
Pseudomonas aeruginosa contains multiple glyoxalase I-encoding genes from both metal activation classes.
Sukdeo N, Honek JF.
Biochim Biophys Acta. 2007 Jun;1774(6):756-63. Epub 2007 Apr 20.
PMID: 17513180
 
Alternate Annotations and Cross-References
TIGR NTL03PA05112
Systomonas PA5111
RefSeq NP_253798.1
GI 15600304
KEGG PA5111
GBrowse Click here for a GBrowse View of this and other annotations.
PseudoCyc Click here to view metabolic pathways in PseudoCyc.


We thank Cystic Fibrosis Foundation Therapeutics, Inc. for critical funding support for the Pseudomonas Genome Database. When acknowledging use of the database, please cite the article by Winsor et al. (2009; PMID:18978025) and not the web site URL. Thank you!

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