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               PA5304               
Key to diagram


View in GBrowse: (?) 17 putative orthologs View metabolic pathways using PseudoCyc Acknowledgements for this annotation
Gene Information
Strain Pseudomonas aeruginosa PAO1 (Tax ID: 208964) Replicon Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name dadA  Alternate Gene Names
Locus tag PA5304 (View 9 updates) GenBank Identifier (GI) 15600497 (Precomputed BLAST results at BLINK )
Genomic location 5973476 - 5972178 (-) Feature type CDS 
Transposon Mutant Available?Transposon mutant library help Yes (View in GBrowse) Pseudomonas Ortholog Group Explanation of Orthologous Groups
POG004876 
Orthology uncertain because more than one top BLASTp hit was identified.
Product Information
Product Name D-amino acid dehydrogenase, small subunit (Class 2  Explanation of Product Name Confidence)
Alternate Product Names
Subcellular localization
Function Classification / Gene Ontology (GO)
PseudoCAP Function Class Energy metabolism
Amino acid biosynthesis and metabolism
GO Accession GO Term Evidence
Molecular Function
GO:0004795 threonine synthase activity IDA
GO:0008718 D-amino-acid dehydrogenase activity RCA
GO:0016647 oxidoreductase activity, acting on the CH-NH group of donors, oxygen as acceptor IEA
Biological Process
GO:0006520 amino acid metabolic process ISS
GO:0006091 generation of precursor metabolites and energy ISS
GO:0006558 L-phenylalanine metabolic process ISS
GO:0006807 nitrogen compound metabolic process ISS
Cellular Component
COG PredictionExplanation of COG predictions
Accession Description Category
COG0665 DadA, Glycine/D-amino acid oxidases (deaminating) [Amino acid transport and metabolism]. Amino acid transport and metabolism
TIGRFAM predictionExplanation of TIGRFAM predictions
Accession Name Function Significance Role Sub Role Release No.
TIGR02352 thiamin_ThiO glycine oxidase ThiO 5.2e-13 Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine 8.0
Pathways
Pathways Explanation of Pathways Field
Name Database Xref
Phenylalanine metabolism PseudoCAP
Nitrogen metabolism PseudoCAP
Phenylalanine metabolism KEGG pae00360 Computationally Generated
Nitrogen metabolism KEGG pae00910 Computationally Generated
Reactions
EC Number 1.4.99.1
Homology
BLINK Precomputed BLAST results at BLINK
Homologs Explanation of Homologs Field 75% similar to dadA gene product of [E. coli]
Most Similar Human Homolog
ENSP00000288050, Percent Identity: 22.32%, E-Value: 6e-11, Ensembl Release: 56.
Description: Pyruvate dehydrogenase phosphatase regulatory subunit, mitochondrial Precursor
Structure Features
Structure Features Explanation of Structure Features Field PS00677: D-amino acid oxidases signature (weak & partial)
type I export signal computationally predicted by LipoP v.1.0 (cleavage after residue 20)
type I export signal computationally predicted by Phobius (cleavage after residue 16)
PFAM predictions Explanation of PFAM predictions
Accession Domain Start Stop E-value Release No.
PF01266 DAO, FAD dependent oxidoreductase. 2 398 2e-56 23.0
PF02737 3HCDH_N, 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain. 2 31 9e-05 23.0
 
Genomic Context
Genomic context Explanation of Genomic Context Field - Near dadX, but unlike E. coli, not adjacent to it.
Operons/Regulons
Type operon
Genes
GeneProduct
PA5303 conserved hypothetical protein
dadA D-amino acid dehydrogenase, small subunit
Evidence Computationally Predicted
PubMed 18988623
Cross-Reference DOOR-14200
Microarray Expression Data
NCBI GEO Link
Sequence data
Upstream 500 BP Region GCGCGGCGTGCCGAATACCAGAAGGTGGCCAACAGCAGCGGCGCTTCGCTGGCCGACGTGGAGGCCATGGCCGGCAAGAAGGCGATGGAGAGAACCCCTG CCGGGCAGTACGTCCAGGTCGGCGGCAAGTGGGTGAAGAAATAGCGCGAAGGCTCCGCGAGCGCTCTGGGACGCTGCTCCGGTCGCAAGGGCCGGGCAGT GTCCGCGGAATTTTTCCTGTCGCGAGCGGTTCCTTCAGGGACTCCTGCCGTTTTCCATGGCTTCTGCGACAGGCTTTCCCGAAAAAGCGACTCACGGCCT GAGTTCGATCATTTAAATATGCGGTATTTTTCCAGTTTTATGGTGGATGCGCCTCGGTAAAACCCCTAATTGTCCGGTTTTTTACCTGAGTAACCGCCTA AAATTGGTGATTATTTCTGCCTGCCTCTTCCTATACTGCGCGCATCTACACGCTCAAAATAACAAACGTCCGCGGTTCCTCCGCGACGTGGGAGGCGACA

BLAST search against this genome or other genomes in database
Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence ATGCGAGTTCTGGTCCTTGGCAGCGGTGTCATCGGTACCGCCAGTGCGTATTACCTGGCCCGTGCCGGGTTCGAGGTGGTGGTGGTCGACCGTCAGGACG GTCCCGCGCTGGAAACCAGCTTCGCCAACGCCGGCCAGGTGTCTCCCGGCTACGCTTCGCCCTGGGCAGCCCCGGGCATTCCCCTGAAGGCCATGAAGTG GCTGCTGGAAAAGCACGCGCCGCTGGCCATCAAGCTCACCTCCGATCCCAGCCAGTACGCCTGGATGCTGCAGATGCTGCGCAACTGCACCGCCGAGCGC TACGCCGTGAACAAGGAGCGCATGGTCCGCCTGTCCGAGTACAGCCGCGATTGCCTCGACGAACTGCGCGCCGAGACCGGCATCGCCTACGAGGGCCGCA CCCTCGGCACCACCCAACTGTTCCGCACCCAGGCGCAGCTGGACGCCGCCGGCAAGGACATCGCCGTGCTCGAGCGCTCCGGCGTGCCCTACGAGGTTCT CGACCGCGACGGCATCGCCCGCGTAGAGCCGGCTTTGGCCAAGGTCGCCGACAAGCTGGTCGGCGCCTTGCGCCTGCCCAACGACCAGACCGGCGACTGC CAGCTGTTCACCACCCGCCTGGCGGAAATGGCCAAGGGCCTGGGCGTGGAGTTCCGCTTCGGCCAGAACATCGAGCGCCTGGACTTCGCCGGCGACCGCA TCAACGGCGTGCTGGTCAACGGCGAATTGCTCACCGCCGACCACTACGTGCTGGCCCTGGGCAGCTACTCGCCGCAACTGCTCAAGCCGCTGGGTATCAA GGCTCCGGTCTATCCGCTGAAGGGTTATTCGCTGACCGTGCCGATCACCAACCCGGAGATGGCGCCGACCTCGACCATCCTCGACGAGACCTACAAGGTG GCGATCACCCGCTTCGACCAGCGCATCCGCGTCGGCGGCATGGCGGAAATCGCCGGCTTCGACCTGTCGCTGAACCCGCGCCGCCGCGAGACCCTGGAAA TGATCACCACCGACCTCTATCCCGAGGGCGGCGATATCAGCCAGGCGACCTTCTGGACCGGCCTGCGCCCGGCGACCCCGGATGGCACCCCGATCGTCGG CGCCACCCGCTACCGCAACCTGTTCCTCAATACCGGCCACGGCACCCTGGGTTGGACCATGGCCTGCGGGTCGGGTCGCTACCTGGCCGACCTGATGGCG AAGAAGCGCCCGCAGATCAGTACCGAAGGCCTGGATATTTCCCGCTACAGCAATTCCCCGGAGAACGCCAAGAATGCCCATCCAGCGCCAGCACACTAA

BLAST search against this genome or other genomes in database


Downstream 500 BP region Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.

Protein Sequence MRVLVLGSGVIGTASAYYLARAGFEVVVVDRQDGPALETSFANAGQVSPGYASPWAAPGIPLKAMKWLLEKHAPLAIKLTSDPSQYAWMLQMLRNCTAER YAVNKERMVRLSEYSRDCLDELRAETGIAYEGRTLGTTQLFRTQAQLDAAGKDIAVLERSGVPYEVLDRDGIARVEPALAKVADKLVGALRLPNDQTGDC QLFTTRLAEMAKGLGVEFRFGQNIERLDFAGDRINGVLVNGELLTADHYVLALGSYSPQLLKPLGIKAPVYPLKGYSLTVPITNPEMAPTSTILDETYKV AITRFDQRIRVGGMAEIAGFDLSLNPRRRETLEMITTDLYPEGGDISQATFWTGLRPATPDGTPIVGATRYRNLFLNTGHGTLGWTMACGSGRYLADLMA KKRPQISTEGLDISRYSNSPENAKNAHPAPAH

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK
References
PubMed Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE.
Genome Res. 2005 Feb;15(2):321-9.
PMID: 15687295
Organization and expression of the Escherichia coli K-12 dad operon encoding the smaller subunit of D-amino acid dehydrogenase and the catabolic alanine racemase.
Lobocka M, Hennig J, Wild J, Klopotowski T.
J Bacteriol. 1994 Mar;176(5):1500-10.
PMID: 7906689
 
Alternate Annotations and Cross-References
TIGR NTL03PA05305
Systomonas PA5304
RefSeq NP_253991.1
GI 15600497
KEGG PA5304
GBrowse Click here for a GBrowse View of this and other annotations.
PseudoCyc Click here to view metabolic pathways in PseudoCyc.


We thank Cystic Fibrosis Foundation Therapeutics, Inc. for critical funding support for the Pseudomonas Genome Database. When acknowledging use of the database, please cite the article by Winsor et al. (2009; PMID:18978025) and not the web site URL. Thank you!

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