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               PA5429               
Key to diagram


View in GBrowse: (?) 16 putative orthologs View metabolic pathways using PseudoCyc Acknowledgements for this annotation
Gene Information
Strain Pseudomonas aeruginosa PAO1 (Tax ID: 208964) Replicon Chromosome(5651 genes, 6264404 bp) [ RefSeq ]
Gene name aspA  Alternate Gene Names
Locus tag PA5429 (View 6 updates) GenBank Identifier (GI) 15600622 (Precomputed BLAST results at BLINK )
Genomic location 6109260 - 6110684 (+) Feature type CDS 
Transposon Mutant Available?Transposon mutant library help Yes (View in GBrowse) Pseudomonas Ortholog Group Explanation of Orthologous Groups
Product Information
Product Name aspartate ammonia-lyase (Class 2  Explanation of Product Name Confidence)
Alternate Product Names aspartase
Subcellular localization
Cytoplasmic (Class 2Explanation of Localization Confidence)
Cytoplasmic (Class 3Explanation of Localization Confidence)
Function Classification / Gene Ontology (GO)
PseudoCAP Function Class Amino acid biosynthesis and metabolism
GO Accession GO Term Evidence
Molecular Function
GO:0003994 aconitate hydratase activity ISS
GO:0008797 aspartate ammonia-lyase activity IEA
Biological Process
GO:0006807 nitrogen compound metabolic process ISS
GO:0006522 alanine metabolic process ISS
GO:0006531 aspartate metabolic process ISS
GO:0006520 amino acid metabolic process IEA
Cellular Component
COG PredictionExplanation of COG predictions
Accession Description Category
COG1027 AspA, Aspartate ammonia-lyase [Amino acid transport and metabolism]. Amino acid transport and metabolism
TIGRFAM predictionExplanation of TIGRFAM predictions
Accession Name Function Significance Role Sub Role Release No.
TIGR00839 aspA aspartate ammonia-lyase 1.2e-275 Energy metabolism Amino acids and amines 8.0
Pathways
Pathways Explanation of Pathways Field
Name Database Xref
Nitrogen metabolism PseudoCAP
Alanine and Aspartate metabolism PseudoCAP
Synthesis of aspartate and asparagine; interconversion of aspartate and asparagine. PseudoCyc ASPASN-PWY
glutamine degradation III PseudoCyc GLUTAMINEFUM-PWY
Alanine, aspartate and glutamate metabolism KEGG pae00250 Computationally Generated
Nitrogen metabolism KEGG pae00910 Computationally Generated
Metabolic pathways KEGG pae01100 Computationally Generated
Reactions
EC Number 4.3.1.1
Enzyme Assays
PDF file(s) provided by Sigma-Aldrich:
 L-Aspartase (4.3.1.1)
Homology
BLINK Precomputed BLAST results at BLINK
Homologs Explanation of Homologs Field 93% similar to aspartate ammonia-lyase [Pseudomonas fluorescens]
Most Similar Human Homolog
ENSP00000355518, Percent Identity: 40.30%, E-Value: 2e-91, Ensembl Release: 56.
Description: Fumarate hydratase, mitochondrial Precursor
Structure Features
Structure Features Explanation of Structure Features Field PS00163: Fumarate lyases signature
PFAM predictions Explanation of PFAM predictions
Accession Domain Start Stop E-value Release No.
PF00206 Lyase_1, Lyase. 15 347 1e-101 23.0
PF10415 FumaraseC_C, Fumarase C C-terminus. 413 467 3e-18 23.0
 
Microarray Expression Data
NCBI GEO Link
Sequence data
Upstream 500 BP Region GGAAGAAGCCCAGCGCCAGCGAGTGGGCGGCGGCGAACTGCAGCACCTCGCCCTGCCCGCCTTCCAGGTGGTGCAGGTGGCGCACCACCTGGCCGAGCTG CTCGACCACGCTGCGCGCGGTGACCAGGAACAGCTGCCCGGCCTCGGTCAGTTCCACCGGCGTGCGCTGGCGATTCACCAGGGTCAGGCCGAGGGCGTCC TCCAGGCTGCGAATGCGCCGGCTGAAGGCCGGCTGGGTGACGAAGCGTTTCTCCGCCGCCTGGGAAAAGCTGCGGGTGCCGGCCAGGGCGATGAAATCCT CCAGCCACTTCAGTTCGAGGTTCACGACGGGAAAGGCTCCGGTAATGCGATGACACGTCCGCCATCATACCAGCGTCACATCCAACATTATGCCGTTTGT GCATGAGGCAGCGTTTAACAGCATTGGCCACGAAATCGTCACATCCTTAGTCTAGTGGGCATCGCGGCACTGGCCGTGTCCCTCTGAGACAAATCCCGTC

BLAST search against this genome or other genomes in database
Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence ATGTCCCCCGTTGCATCTTCCCGTATCGAGAAAGACCTGCTCGGCACCCTCGAAGTGCCCGCTGACGCCTACTACGGCATCCAGACCCTGCGCGCCGTGA ACAACTTCCGCCTCTCCGGGGTGCCGCTGTCGCACTACCCGAAGCTGGTCGTTGCCCTGGCCATGGTCAAGCAGGCCGCCGCCGACGCCAACCGTCAGCT CGGCCATCTCCCGGAAGACAAGCACGCCGCCATCAGCGAGGCCTGCGCCCGCCTGATCCGCGGCGATTTCCACGAGCAGTTCGTGGTGGACATGATCCAG GGCGGCGCCGGCACCTCCACCAACATGAACGCGAACGAAGTGATCGCGAACATCGCGCTGGAAGCCATGGGCCACACCAAGGGTGAGTACAAGTACCTGC ACCCGAACAACGATGTGAACATGGCGCAGTCGACCAACGACGCCTACCCAACCGCCATCCGCCTGGGCCTGCTGCTCGGCCACGACACCCTGCTGGCCAG CCTCGACAGCCTGATCCAGGCCTTCGCCGCCAAGGGCGTCGAATTCGCCGGCGTACTGAAGATGGGCCGCACCCAGTTGCAGGACGCCGTGCCGATGACC CTCGGCCAGGAATTCCACGCTTTCGCCACCACCCTCGGCGAAGACCTCGACCGCCTGCGTCGCCTGGCGCCGGAGCTGCTCACCGAAGTGAACCTCGGTG GCACCGCCATCGGCACCGGCATCAACGCCGACCCCGGCTACCAGAAGCTCGCCGTCGAGCGCCTGGCCGCCATCAGCGGGCAGCCGCTGAAGCCGGCCGC CGACCTGATCGAAGCCACCTCCGACATGGGCGCCTTCGTGCTGTTCTCCGGCATGCTCAAGCGCACCGCGGTCAAGCTGTCGAAGATCTGCAACGACCTG CGCCTGCTCTCCAGCGGCCCGCGCACCGGCATCAACGAGATCAACCTGCCGCCGCGCCAGCCGGGCAGTTCGATCATGCCGGGCAAGGTCAACCCGGTGA TCCCGGAAGCGGTCAACCAGGTCGCCTTCGAAGTGATCGGCAACGACCTCGCCCTGACCCTGGCGGCCGAAGGCGGCCAGTTGCAACTGAACGTGATGGA GCCGCTGATCGCCTACAAGATCTTCGACTCGATCCGCCTGCTGCAACGCGCCATGGACATGCTCCGCGAGCACTGCATCACCGGCATCACCGCCAACGTC GAGCGCTGCCACGAACTGGTCGAGCACAGCATCGGTCTGGTCACCGCGCTGAACCCGTACATCGGCTACGAGAACTCCACGCGGATCGCCAAGACCGCCC TGGAGAGCGGCCGCGGCGTCCTGGAACTGGTACGCGAGGAAAAGCTGCTGGACGAAGCCACCCTGGCCGACATCCTGCTGCCGGAAAACATGATCGCGCC GCGCCTGATTCCGCTGCGCGCCTGA

BLAST search against this genome or other genomes in database


Downstream 500 BP region Click here to view the nucleotide sequence of the region 500 BP downstream of this gene.

Protein Sequence MSPVASSRIEKDLLGTLEVPADAYYGIQTLRAVNNFRLSGVPLSHYPKLVVALAMVKQAAADANRQLGHLPEDKHAAISEACARLIRGDFHEQFVVDMIQ GGAGTSTNMNANEVIANIALEAMGHTKGEYKYLHPNNDVNMAQSTNDAYPTAIRLGLLLGHDTLLASLDSLIQAFAAKGVEFAGVLKMGRTQLQDAVPMT LGQEFHAFATTLGEDLDRLRRLAPELLTEVNLGGTAIGTGINADPGYQKLAVERLAAISGQPLKPAADLIEATSDMGAFVLFSGMLKRTAVKLSKICNDL RLLSSGPRTGINEINLPPRQPGSSIMPGKVNPVIPEAVNQVAFEVIGNDLALTLAAEGGQLQLNVMEPLIAYKIFDSIRLLQRAMDMLREHCITGITANV ERCHELVEHSIGLVTALNPYIGYENSTRIAKTALESGRGVLELVREEKLLDEATLADILLPENMIAPRLIPLRA

BLAST search against this genome or other genomes in database
View precomputed BLAST results against all genomes at NCBI BLINK
References
PubMed Cloning and nucleotide sequence of the aspartase gene of Pseudomonas fluorescens.
Takagi JS, Tokushige M, Shimura Y.
J Biochem. 1986 Sep;100(3):697-705.
PMID: 3096982
 
Alternate Annotations and Cross-References
TIGR NTL03PA05430
Systomonas PA5429
RefSeq NP_254116.1
GI 15600622
KEGG PA5429
GBrowse Click here for a GBrowse View of this and other annotations.
PseudoCyc Click here to view metabolic pathways in PseudoCyc.


We thank Cystic Fibrosis Foundation Therapeutics, Inc. for critical funding support for the Pseudomonas Genome Database. When acknowledging use of the database, please cite the article by Winsor et al. (2009; PMID:18978025) and not the web site URL. Thank you!

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