Pseudomonas aeruginosa PAO1, PA0423 (pasP)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
Locus Tag
Synonym: pasP
Synonym: yceI
Replicon chromosome
Genomic location 470662 - 471237 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs


RefSeq NP_249114.1
GI 15595620
Affymetrix PA0423_at
Entrez 877902
GenBank AAG03812.1
INSDC AAG03812.1
NCBI Locus Tag PA0423
protein_id(GenBank) gb|AAG03812.1|AE004479_7|gnl|PseudoCAP|PA0423
UniParc UPI00000C504B
UniProtKB Acc Q9I690
UniRef100 UniRef100_B7V411
UniRef50 UniRef50_Q32E67
UniRef90 UniRef90_B7V411


Feature Type CDS
Coding Frame 1
Product Name
Product Name Confidence: Class 1
Synonyms PasP
Evidence for Translation
Detected using LC-MS/MS (20.78 kDa, PMID:21751344)
Verified using two-dimensional gel electrophoresis, MALDI-TOF MS and peptide mass mapping(pI:6.1, molecular weight:20.8, PMID:21674802)
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -1.04
Kyte-Doolittle Hydrophobicity Value -0.380
Molecular Weight (kDa) 20.8
Isoelectric Point (pI) 6.53

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 1
Unknown Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7BWL ANTIBIOTIC 04/14/20 Structure of antibiotic sequester from Pseudomonas aerurinosa Pseudomonas aeruginosa PAO1 2.25 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database PseudoCAP
Category Pseudomonas aeruginosa small protease (PASP)
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Mus musculus
Infection model Mouse scratch model of P. aeruginosa keratitis
Reference 23548618
Source 2
Database PseudoCAP
Category Pseudomonas aeruginosa small protease (PASP)
Evidence ECO:0000314
direct assay evidence used in manual assertion
Host Organism Oryctolagus cuniculus
Infection model P. aeruginosa corneal infection of New Zealand white rabbit
Reference 16186360

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 310 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000414 (536 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA0423
Search term: pasP
Search term: PasP

Human Homologs


Identification of a novel secreted protease from Pseudomonas aeruginosa that causes corneal erosions.
Marquart ME, Caballero AR, Chomnawang M, Thibodeaux BA, Twining SS, O'Callaghan RJ
Invest Ophthalmol Vis Sci 2005 Oct;46(10):3761-8
PubMed ID: 16186360
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomics of the oxidative stress response induced by hydrogen peroxide and paraquat reveals a novel AhpC-like protein in Pseudomonas aeruginosa.
Hare NJ, Scott NE, Shin EH, Connolly AM, Larsen MR, Palmisano G, Cordwell SJ
Proteomics 2011 Aug;11(15):3056-69
PubMed ID: 21674802
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
Mep72, a metzincin protease that is preferentially secreted by biofilms of Pseudomonas aeruginosa.
Passmore IJ, Nishikawa K, Lilley KS, Bowden SD, Chung JC, Welch M
J Bacteriol 2015 Feb 15;197(4):762-73
PubMed ID: 25488299