Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA0958
|
Name |
oprD
|
Replicon | chromosome |
Genomic location | 1043983 - 1045314 (- strand) |
Transposon Mutants | 4 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 7 transposon mutants in orthologs |
Comment | Localication to outer membrane vesicle confirmed in three separate studies (PMID:22909304, PMID:21751344 and PMID:26303878). |
RefSeq | NP_249649.1 |
GI | 15596155 |
Affymetrix | PA0958_oprD_at |
Entrez | 881970 |
GenBank | AAG04347.1 |
INSDC | AAG04347.1 |
NCBI Locus Tag | PA0958 |
protein_id(GenBank) | gb|AAG04347.1|AE004529_13|gnl|PseudoCAP|PA0958 |
TIGR | NTL03PA00959 |
UniParc | UPI0000131F72 |
UniProtKB Acc | P32722 |
UniProtKB ID | PORD_PSEAE |
UniRef100 | UniRef100_P32722 |
UniRef50 | UniRef50_P32722 |
UniRef90 | UniRef90_P32722 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
Basic amino acid, basic peptide and imipenem outer membrane porin OprD precursor
|
Synonyms |
Porin D Protein D2 OccD1 |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (48.36 kDa, PMID:21751344)
Detected by MALDI-TOF/TOF (PMID:19333994).
Detected using 2-DE, MALDI-TOF/MS analysis (47.98 kDa, pI 4.9, PMID:16335974)
|
Charge (pH 7) | -12.55 |
Kyte-Doolittle Hydrophobicity Value | -0.466 |
Molecular Weight (kDa) | 48.4 |
Isoelectric Point (pI) | 4.75 |
Individual Mappings | |
Additional evidence for subcellular localization |
Detected in sarcosine-insoluble outer membrane fraction using 2-DE, MALDI-TOF/MS analysis (PMID:16335974)
|
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
2ODJ | MEMBRANE PROTEIN | 12/22/06 | Crystal structure of the outer membrane protein OprD from Pseudomonas aeruginosa | Pseudomonas aeruginosa | 2.9 | X-RAY DIFFRACTION | 100.0 |
4FOZ | PROTEIN TRANSPORT | 06/21/12 | Crystal Structure of OccD1 (OprD) Y282R/D307H | Pseudomonas aeruginosa | 2.4 | X-RAY DIFFRACTION | 99.3 |
3SY7 | MEMBRANE PROTEIN | 07/15/11 | Improved crystal structure of Pseudomonas aeruginosa OprD | Pseudomonas aeruginosa | 2.15 | X-RAY DIFFRACTION | 99.8 |
Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.
Drug Name | Source Accession | Source DB | Version | Target Accession | Target Description | Percent Identity | Alignment Length | E-Value |
(Hydroxyethyloxy)Tri(Ethyloxy)Octane | DB04233 | DrugBank | 5.1.4 | P32722 | Porin D | 100.0 | 443 | 6.2e-259 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 16 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG000187 (1579 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA0958
Search term: oprD
|
Identification of porins in the outer membrane of Pseudomonas aeruginosa that form small diffusion pores.
Yoshihara E, Nakae T
J Biol Chem 1989 Apr 15;264(11):6297-301
PubMed ID: 2539376
|
Proteomic analysis of the sarcosine-insoluble outer membrane fraction of Pseudomonas aeruginosa responding to ampicilin, kanamycin, and tetracycline resistance.
Peng X, Xu C, Ren H, Lin X, Wu L, Wang S
J Proteome Res 2005 Nov-Dec;4(6):2257-65
PubMed ID: 16335974
|
Protein D2 channel of the Pseudomonas aeruginosa outer membrane has a binding site for basic amino acids and peptides.
Trias J, Nikaido H
J Biol Chem 1990 Sep 15;265(26):15680-4
PubMed ID: 2118530
|
Nucleotide sequence of the protein D2 gene of Pseudomonas aeruginosa.
Yoneyama H, Yoshihara E, Nakae T
Antimicrob Agents Chemother 1992 Aug;36(8):1791-3
PubMed ID: 1339257
|
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
|
Proteomic analysis of the sarcosine-insoluble outer membrane fraction of Pseudomonas aeruginosa responding to ampicilin, kanamycin, and tetracycline resistance.
Peng X, Xu C, Ren H, Lin X, Wu L, Wang S
J Proteome Res 2005 Nov-Dec;4(6):2257-65
PubMed ID: 16335974
|
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
|
Identification of proteins associated with the Pseudomonas aeruginosa biofilm extracellular matrix.
Toyofuku M, Roschitzki B, Riedel K, Eberl L
J Proteome Res 2012 Oct 5;11(10):4906-15
PubMed ID: 22909304
|
Proteome Profiles of Outer Membrane Vesicles and Extracellular Matrix of Pseudomonas aeruginosa Biofilms.
Couto N, Schooling SR, Dutcher JR, Barber J
J Proteome Res 2015 Oct 2;14(10):4207-22
PubMed ID: 26303878
|
Responses of carbapenemase-producing and non-producing carbapenem-resistant Pseudomonas aeruginosa strains to meropenem revealed by quantitative tandem mass spectrometry proteomics.
Salvà-Serra F, Jaén-Luchoro D, Marathe NP, Adlerberth I, Moore ERB, Karlsson R
Front Microbiol 2022;13:1089140
PubMed ID: 36845973
|
OccK channels from Pseudomonas aeruginosa exhibit diverse single-channel electrical signatures but conserved anion selectivity.
Liu J, Eren E, Vijayaraghavan J, Cheneke BR, Indic M, van den Berg B, Movileanu L
Biochemistry 2012 Mar 20;51(11):2319-30
PubMed ID: 22369314
|
High-level resistance to meropenem in clinical isolates of Pseudomonas aeruginosa in the absence of carbapenemases: role of active efflux and porin alterations.
Chalhoub H, Sáenz Y, Rodriguez-Villalobos H, Denis O, Kahl BC, Tulkens PM, Van Bambeke F
Int J Antimicrob Agents 2016 Dec;48(6):740-743
PubMed ID: 28128097
|