Pseudomonas aeruginosa PAO1, PA1010 (dapA)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
Locus Tag
Replicon chromosome
Genomic location 1093251 - 1094129 (+ strand)


RefSeq NP_249701.1
GI 15596207
Affymetrix PA1010_dapA_at
Entrez 879525
GenBank AAG04399.1
INSDC AAG04399.1
NCBI Locus Tag PA1010
protein_id(GenBank) gb|AAG04399.1|AE004533_10|gnl|PseudoCAP|PA1010
UniParc UPI0000128E58
UniProtKB Acc Q9I4W3
UniRef100 UniRef100_Q9I4W3
UniRef50 UniRef50_Q9JZR4
UniRef90 UniRef90_Q9I4W3


Feature Type CDS
Coding Frame 1
Product Name
dihydrodipicolinate synthase
Product Name Confidence: Class 2
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Charge (pH 7) -2.44
Kyte-Doolittle Hydrophobicity Value 0.043
Molecular Weight (kDa) 31.4
Isoelectric Point (pI) 6.40

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3PUO LYASE 12/06/10 Crystal structure of dihydrodipicolinate synthase from Pseudomonas aeruginosa(PsDHDPS)complexed with L-lysine at 2.65A resolution Pseudomonas aeruginosa 2.65 X-RAY DIFFRACTION 98.6
3PS7 LYASE 12/01/10 Biochemical studies and crystal structure determination of dihydrodipicolinate synthase from Pseudomonas aeruginosa Pseudomonas aeruginosa 2.85 X-RAY DIFFRACTION 98.6
3S8H LYASE 05/28/11 Structure of dihydrodipicolinate synthase complexed with 3-Hydroxypropanoic acid(HPA)at 2.70 A resolution Pseudomonas aeruginosa 2.7 X-RAY DIFFRACTION 98.6
6P90 STRUCTURAL PROTEIN 06/08/19 Crystal structure of PaDHDPS2-H56Q mutant Pseudomonas aeruginosa 1.9 X-RAY DIFFRACTION 99.7
3QZE LYASE 03/05/11 Crystal Structure of DapA (PA1010) at 1.6 A resolution Pseudomonas aeruginosa 1.59 X-RAY DIFFRACTION 100.0
3NOE LYASE 06/25/10 Crystal Structure of Dihydrodipicolinate synthase from Pseudomonas aeruginosa Pseudomonas aeruginosa 2.95 X-RAY DIFFRACTION 98.6

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 633 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000973 (536 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA1010
Search term: dapA

Human Homologs


Reaction mechanism of Escherichia coli dihydrodipicolinate synthase investigated by X-ray crystallography and NMR spectroscopy.
Blickling S, Renner C, Laber B, Pohlenz HD, Holak TA, Huber R
Biochemistry 1997 Jan 7;36(1):24-33
PubMed ID: 8993314
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142