Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA3724
|
Name |
lasB
|
Replicon | chromosome |
Genomic location | 4168987 - 4170483 (- strand) |
Transposon Mutants | 4 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 2 transposon mutants in orthologs |
RefSeq | NP_252413.1 |
GI | 15598919 |
Affymetrix | PA3724_lasB_at |
Entrez | 880368 |
GenBank | AAG07111.1 |
NCBI Locus Tag | PA3724 |
protein_id(GenBank) | gb|AAG07111.1|AE004791_8|gnl|PseudoCAP|PA3724 |
TIGR | NTL03PA03724 |
UniProtKB Acc | P14756 |
UniProtKB ID | ELAS_PSEAE |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
elastase LasB
|
Synonyms |
neutral metalloproteinase pseudolysin precursor |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | -1.48 |
Kyte-Doolittle Hydrophobicity Value | -0.271 |
Molecular Weight (kDa) | 53.7 |
Isoelectric Point (pI) | 6.74 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
4K89 | HYDROLASE | 04/18/13 | Crystal structure of Pseudomonas aeruginosa strain K solvent tolerant elastase | Pseudomonas aeruginosa | 1.39 | X-RAY DIFFRACTION | 99.7 |
1EZM | HYDROLASE | 01/13/92 | THREE-DIMENSIONAL STRUCTURE OF THE ELASTASE OF PSEUDOMONAS AERUGINOSA AT 1.5 ANGSTROMS RESOLUTION | Pseudomonas aeruginosa | 1.5 | X-RAY DIFFRACTION | 100.0 |
7NLK | HYDROLASE | 02/22/21 | LasB, N-aryl-2-butylmercaptoacetamide | Pseudomonas aeruginosa (strain UCBPP-PA14) | 1.7 | X-RAY DIFFRACTION | 98.7 |
6F8B | HYDROLASE | 12/12/17 | LasB bound to thiol based inhibitor | Pseudomonas aeruginosa | 1.3 | X-RAY DIFFRACTION | 100.0 |
7AJR | HYDROLASE | 09/29/20 | Virtual screening approach leading to the identification of a novel and tractable series of Pseudomonas aeruginosa elastase inhibitors | Pseudomonas aeruginosa | 1.75 | X-RAY DIFFRACTION | 100.0 |
7QH1 | HYDROLASE | 12/10/21 | Discovery and development of a novel inhaled antivirulence therapy for the treatment of Pseudomonas aeruginosa infections in patients with chronic respiratory disease | Pseudomonas aeruginosa | 2.74 | X-RAY DIFFRACTION | 100.0 |
1U4G | HYDROLASE | 07/25/04 | Elastase of Pseudomonas aeruginosa with an inhibitor | Pseudomonas aeruginosa | 1.4 | X-RAY DIFFRACTION | 100.0 |
8CR7 | HYDROLASE | 03/07/23 | Crystal structure of recombinant LasB from Pseudomonas aeruginosa PA7 | Pseudomonas aeruginosa PA7 | 1.5 | X-RAY DIFFRACTION | 95.9 |
6FZX | HYDROLASE | 03/15/18 | LasB, hydroxymate Inhibitor Complex | Pseudomonas aeruginosa | 2.1 | X-RAY DIFFRACTION | 100.0 |
3DBK | HYDROLASE | 06/01/08 | Pseudomonas aeruginosa elastase with phosphoramidon | Pseudomonas aeruginosa | 1.4 | X-RAY DIFFRACTION | 100.0 |
8CR4 | HYDROLASE | 03/07/23 | Crystal structure of recombinant LasBArtif from Pseudomonas aeruginosa AZPAE14816 | Pseudomonas aeruginosa | 0.91 | X-RAY DIFFRACTION | 94.9 |
7OC7 | HYDROLASE | 04/26/21 | LasB, alpha-alkyl-N-aryl mercaptoacetamide | Pseudomonas aeruginosa | 1.95 | X-RAY DIFFRACTION | 98.7 |
7NLM | HYDROLASE | 02/22/21 | LasB, N-aryl-2-butylmercaptoacetamide | Pseudomonas aeruginosa (strain UCBPP-PA14) | 1.65 | X-RAY DIFFRACTION | 98.7 |
8CR3 | HYDROLASE | 03/07/23 | Crystal structure of recombinant LasB from Pseudomonas aeruginosa PAO1 | Pseudomonas aeruginosa PAO1 | 1.12 | X-RAY DIFFRACTION | 98.4 |
Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.
Drug Name | Source Accession | Source DB | Version | Target Accession | Target Description | Percent Identity | Alignment Length | E-Value |
N-(1-Carboxy-3-Phenylpropyl)Phenylalanyl-Alpha-Asparagine | DB02307 | DrugBank | 5.1.4 | P14756 | Elastase | 100.0 | 498 | 6.5e-289 |
Source 1 | |
Database | PseudoCAP |
Category | Elastase |
Evidence |
ECO:0000315
mutant phenotype evidence used in manual assertion
|
Host Organism | Oryctolagus cuniculus |
Notes | LasB mutation reduced invasion of rabbit corneal epithelial (RCE) cells by 70% |
Reference | 12904569 |
Source 2 | |
Database | PseudoCAP |
Category | Elastase |
Evidence |
ECO:0000315
mutant phenotype evidence used in manual assertion
|
Host Organism | Cavia porcellus |
Notes | |
Infection model | Guinea pig acute lung infection |
Reference | 6401692 |
Source 3 | |
Database | VFDB : VF0087 |
Category | Elastase |
Evidence |
|
Host Organism | |
Notes | |
Reference |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 114 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG001959 (310 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA3724
Search term: lasB
Search term: elastase LasB
|
Characterization and purification of an outer membrane metalloproteinase from Pseudomonas aeruginosa with fibrinogenolytic activity.
Fricke B, Parchmann O, Kruse K, Rücknagel P, Schierhorn A, Menge S
Biochim Biophys Acta 1999 Aug 30;1454(3):236-50
PubMed ID: 10452958
|
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
|
Molecular characterization and nucleotide sequence of the Pseudomonas aeruginosa elastase structural gene.
Bever RA, Iglewski BH
J Bacteriol 1988 Sep;170(9):4309-14
PubMed ID: 2842313
|
Three-dimensional structure of the elastase of Pseudomonas aeruginosa at 1.5-A resolution.
Thayer MM, Flaherty KM, McKay DB
J Biol Chem 1991 Feb 15;266(5):2864-71
PubMed ID: 1899664
|
Efficient production and processing of elastase and LasA by Pseudomonas aeruginosa require zinc and calcium ions.
Olson JC, Ohman DE
J Bacteriol 1992 Jun;174(12):4140-7
PubMed ID: 1597429
|
Secretion of elastinolytic enzymes and their propeptides by Pseudomonas aeruginosa.
Braun P, de Groot A, Bitter W, Tommassen J
J Bacteriol 1998 Jul;180(13):3467-9
PubMed ID: 9642203
|