Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA4270
|
Name |
rpoB
|
Replicon | chromosome |
Genomic location | 4776544 - 4780617 (- strand) |
Comment | Localication to outer membrane vesicle confirmed in three separate studies (PMID:22909304, PMID:21751344 and PMID:26303878). |
RefSeq | NP_252960.1 |
GI | 15599466 |
Affymetrix | PA4270_rpoB_at |
Entrez | 881699 |
GenBank | AAG07658.1 |
INSDC | AAA25985.1 |
NCBI Locus Tag | PA4270 |
protein_id(GenBank) | gb|AAG07658.1|AE004842_7|gnl|PseudoCAP|PA4270 |
TIGR | NTL03PA04271 |
UniParc | UPI00001346F1 |
UniProtKB Acc | Q51561 |
UniProtKB ID | RPOB_PSEAE |
UniRef100 | UniRef100_Q51561 |
UniRef50 | UniRef50_P06173 |
UniRef90 | UniRef90_Q51561 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
DNA-directed RNA polymerase beta chain
|
Synonyms | |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Detected using LC-MS/MS (150.84 kDa, PMID:21751344)
|
Charge (pH 7) | -19.87 |
Kyte-Doolittle Hydrophobicity Value | -0.286 |
Molecular Weight (kDa) | 150.8 |
Isoelectric Point (pI) | 5.58 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
7XL3 | TRANSCRIPTION | 04/21/22 | Cryo-EM structure of Pseudomonas aeruginosa RNAP sigmaS holoenzyme complexes with transcription factor SutA (open lobe) | Pseudomonas aeruginosa PAO1 | 3.13 | ELECTRON MICROSCOPY | 100.0 |
7F0R | TRANSCRIPTION/DNA | 06/06/21 | Cryo-EM structure of Pseudomonas aeruginosa SutA transcription activation complex | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1); SYNTHETIC CONSTRUCT | 5.8 | ELECTRON MICROSCOPY | 100.0 |
7XL4 | TRANSCRIPTION | 04/21/22 | Cryo-EM structure of Pseudomonas aeruginosa RNAP sigmaS holoenzyme complexes with transcription factor SutA (closed lobe) | Pseudomonas aeruginosa PAO1 | 3.86 | ELECTRON MICROSCOPY | 100.0 |
7XYA | TRANSCRIPTION | 06/01/22 | The cryo-EM structure of an AlpA-loading complex | Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT | 3.3 | ELECTRON MICROSCOPY | 100.0 |
7VF9 | TRANSCRIPTION | 09/10/21 | Cryo-EM structure of Pseudomonas aeruginosa RNAP sigmaS holoenzyme complexes | Pseudomonas aeruginosa PAO1 | 4.04 | ELECTRON MICROSCOPY | 100.0 |
7XYB | TRANSCRIPTION | 06/01/22 | The cryo-EM structure of an AlpA-loaded complex | Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT | 3.7 | ELECTRON MICROSCOPY | 100.0 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 667 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG001441 (533 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA4270
Search term: rpoB
Search term: DNA-directed RNA polymerase beta chain
|
The primary structure of Escherichia coli RNA polymerase. Nucleotide sequence of the rpoB gene and amino-acid sequence of the beta-subunit.
Ovchinnikov YA, Monastyrskaya GS, Gubanov VV, Guryev SO, Chertov OYu, Modyanov NN, Grinkevich VA, Makarova IA, Marchenko TV, Polovnikova IN, Lipkin VM, Sverdlov ED
Eur J Biochem 1981 Jun 1;116(3):621-9
PubMed ID: 6266829
|
A beta subunit mutation disrupting the catalytic function of Escherichia coli RNA polymerase.
Lee J, Kashlev M, Borukhov S, Goldfarb A
Proc Natl Acad Sci U S A 1991 Jul 15;88(14):6018-22
PubMed ID: 2068078
|
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
|
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
|
Identification of proteins associated with the Pseudomonas aeruginosa biofilm extracellular matrix.
Toyofuku M, Roschitzki B, Riedel K, Eberl L
J Proteome Res 2012 Oct 5;11(10):4906-15
PubMed ID: 22909304
|
Proteome Profiles of Outer Membrane Vesicles and Extracellular Matrix of Pseudomonas aeruginosa Biofilms.
Couto N, Schooling SR, Dutcher JR, Barber J
J Proteome Res 2015 Oct 2;14(10):4207-22
PubMed ID: 26303878
|