Pseudomonas aeruginosa PAO1, PA5203 (gshA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Ontology

Ontology Accession Term GO Evidence Evidence Ontology (ECO) Code Reference Comments
Molecular Function GO:0004357 glutamate-cysteine ligase activity
ISS
Inferred from Sequence or Structural Similarity 		ECO:0000250
sequence similarity evidence used in manual assertion		 2895925 Reviewed by curator
Biological Process GO:0006750 glutathione biosynthetic process
ISS
Inferred from Sequence or Structural Similarity 		ECO:0000250
sequence similarity evidence used in manual assertion		 2895925 Reviewed by curator
Molecular Function GO:0003824 catalytic activity
ISM
Inferred from Sequence Model
Term mapped from: InterPro:SSF55931
 ECO:0000259
match to InterPro signature evidence used in automatic assertion
Molecular Function GO:0004357 glutamate-cysteine ligase activity
ISM
Inferred from Sequence Model
Term mapped from: InterPro:TIGR01434
 ECO:0000259
match to InterPro signature evidence used in automatic assertion
Biological Process GO:0006750 glutathione biosynthetic process
ISM
Inferred from Sequence Model
Term mapped from: InterPro:TIGR01434
 ECO:0000259
match to InterPro signature evidence used in automatic assertion

Functional Classifications Manually Assigned by PseudoCAP

Amino acid biosynthesis and metabolism Other PAO1 genes in this class
Biosynthesis of cofactors, prosthetic groups and carriers Other PAO1 genes in this class

Pathways

Database Xref Pathway Version Evidence PMID
PseudoCAP Glutamate metabolism ECO:0000037
not_recorded
KEGG pae00270 Cysteine and methionine metabolism 81.0+/01-23, Jan 17 ECO:0000249
sequence similarity evidence used in automatic assertion
KEGG pae01100 Metabolic pathways 81.0+/01-23, Jan 17 ECO:0000249
sequence similarity evidence used in automatic assertion
KEGG pae00480 Glutathione metabolism 81.0+/01-23, Jan 17 ECO:0000249
sequence similarity evidence used in automatic assertion
PseudoCyc GLUTATHIONESYN-PWY glutathione biosynthesis 19.5 ECO:0000250
sequence similarity evidence used in manual assertion 		12867747
PseudoCAP Glutathione metabolism ECO:0000037
not_recorded

Functional Predictions from Interpro

Analysis Accession Description Interpro Accession Interpro Description Amino Acid Start Amino Acid Stop E-value
PANTHER PTHR38761 GLUTAMATE--CYSTEINE LIGASE IPR006334 Glutamate--cysteine ligase, monofunctional 1 520 0.0
Hamap MF_00578 Glutamate--cysteine ligase [gshA]. IPR006334 Glutamate--cysteine ligase, monofunctional 1 527 229.449738
SUPERFAMILY SSF55931 Glutamine synthetase/guanido kinase IPR014746 Glutamine synthetase/guanido kinase, catalytic domain 8 514 0.0
Gene3D G3DSA:3.30.590.20 - - - 21 523 0.0
NCBIfam TIGR01434 JCVI: glutamate--cysteine ligase IPR006334 Glutamate--cysteine ligase, monofunctional 6 517 0.0
FunFam G3DSA:3.30.590.20:FF:000007 Glutamate--cysteine ligase - - 21 523 0.0
Pfam PF04262 Glutamate-cysteine ligase IPR007370 Glutamate--cysteine ligase 10 378 0.0

Search for additional functional domains at the NCBI CDD database website. Go to this protein's amino acid sequence and follow the link.