Pseudomonas fluorescens SBW25, PFLU4748 (alaS)
Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
Gene Ontology
| Ontology | Accession | Term | GO Evidence | Evidence Ontology (ECO) Code | Reference | Comments |
|---|---|---|---|---|---|---|
| Molecular Function | GO:0004812 | aminoacyl-tRNA ligase activity |
Inferred from Sequence Model
Term mapped from: InterPro:SM00863
|
ECO:0000259 match to InterPro signature evidence used in automatic assertion |
||
| Biological Process | GO:0006419 | alanyl-tRNA aminoacylation |
Inferred from Sequence Model
Term mapped from: InterPro:SSF101353
|
ECO:0000259 match to InterPro signature evidence used in automatic assertion |
||
| Molecular Function | GO:0005524 | ATP binding |
Inferred from Sequence Model
Term mapped from: InterPro:SSF101353
|
ECO:0000259 match to InterPro signature evidence used in automatic assertion |
||
| Molecular Function | GO:0004813 | alanine-tRNA ligase activity |
Inferred from Sequence Model
Term mapped from: InterPro:SSF101353
|
ECO:0000259 match to InterPro signature evidence used in automatic assertion |
||
| Molecular Function | GO:0000166 | nucleotide binding |
Inferred from Sequence Model
Term mapped from: InterPro:SSF55186
|
ECO:0000259 match to InterPro signature evidence used in automatic assertion |
||
| Molecular Function | GO:0003676 | nucleic acid binding |
Inferred from Sequence Model
Term mapped from: InterPro:PF02272
|
ECO:0000259 match to InterPro signature evidence used in automatic assertion |
||
| Cellular Component | GO:0005737 | cytoplasm |
Inferred from Sequence Model
Term mapped from: InterPro:SSF101353
|
ECO:0000259 match to InterPro signature evidence used in automatic assertion |
||
| Biological Process | GO:0043039 | tRNA aminoacylation |
Inferred from Sequence Model
Term mapped from: InterPro:SM00863
|
ECO:0000259 match to InterPro signature evidence used in automatic assertion |
Functional Classifications Manually Assigned by PseudoCAP
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Pathways
| Database | Xref | Pathway | Version | Evidence | PMID |
|---|---|---|---|---|---|
| KEGG (InterPro) | 00970 | Aminoacyl-tRNA biosynthesis | InterPro 5.8-49.0 |
ECO:0000259
match to InterPro signature evidence used in automatic assertion |
0 |
Functional Predictions from Interpro
| Analysis | Accession | Description | Interpro Accession | Interpro Description | Amino Acid Start | Amino Acid Stop | E-value |
|---|---|---|---|---|---|---|---|
| FunFam | G3DSA:3.30.54.20:FF:000001 | Alanine--tRNA ligase | - | - | 620 | 674 | 5.2E-26 |
| Gene3D | G3DSA:6.10.250.550 | - | - | - | 697 | 759 | 2.1E-7 |
| Coils | Coil | Coil | - | - | 704 | 756 | - |
| SUPERFAMILY | SSF50447 | Translation proteins | IPR009000 | Translation protein, beta-barrel domain superfamily | 450 | 548 | 4.24E-19 |
| Gene3D | G3DSA:3.30.54.20 | - | - | - | 620 | 674 | 1.3E-54 |
| FunFam | G3DSA:3.30.980.10:FF:000004 | Alanine--tRNA ligase, cytoplasmic | - | - | 549 | 708 | 2.6E-40 |
| FunFam | G3DSA:3.30.930.10:FF:000004 | Alanine--tRNA ligase | - | - | 1 | 248 | 2.8E-126 |
| Gene3D | G3DSA:2.40.30.130 | - | - | - | 453 | 548 | 1.3E-16 |
| Gene3D | G3DSA:3.30.930.10 | Bira Bifunctional Protein; Domain 2 | IPR045864 | Class II Aminoacyl-tRNA synthetase/Biotinyl protein ligase (BPL) and lipoyl protein ligase (LPL) | 1 | 248 | 7.7E-119 |
| SUPERFAMILY | SSF55681 | Class II aaRS and biotin synthetases | IPR045864 | Class II Aminoacyl-tRNA synthetase/Biotinyl protein ligase (BPL) and lipoyl protein ligase (LPL) | 2 | 251 | 2.98E-105 |
| PANTHER | PTHR11777 | ALANYL-TRNA SYNTHETASE | - | - | 1 | 863 | 0.0 |
| PRINTS | PR00980 | Alanyl-tRNA synthetase signature | IPR002318 | Alanine-tRNA ligase, class IIc | 302 | 315 | 2.0E-29 |
| SMART | SM00863 | tRNA_SAD_4 | IPR012947 | Threonyl/alanyl tRNA synthetase, SAD | 649 | 693 | 1.5E-17 |
| SUPERFAMILY | SSF55186 | ThrRS/AlaRS common domain | IPR018163 | Threonyl/alanyl tRNA synthetase, class II-like, putative editing domain superfamily | 548 | 709 | 4.45E-49 |
| PRINTS | PR00980 | Alanyl-tRNA synthetase signature | IPR002318 | Alanine-tRNA ligase, class IIc | 74 | 85 | 2.0E-29 |
| Gene3D | G3DSA:3.10.310.40 | - | - | - | 761 | 868 | 1.5E-32 |
| SUPERFAMILY | SSF101353 | Putative anticodon-binding domain of alanyl-tRNA synthetase (AlaRS) | IPR018162 | Alanine-tRNA ligase, class IIc, anti-codon-binding domain superfamily | 252 | 458 | 2.09E-59 |
| Gene3D | G3DSA:3.30.980.10 | - | - | - | 559 | 695 | 1.3E-54 |
| Pfam | PF01411 | tRNA synthetases class II (A) | IPR018164 | Alanyl-tRNA synthetase, class IIc, N-terminal | 5 | 551 | 0.0 |
| Hamap | MF_00036_B | Alanine--tRNA ligase [alaS]. | IPR023033 | Alanine-tRNA ligase, eukaryota/bacteria | 1 | 872 | 201.229752 |
| Pfam | PF07973 | Threonyl and Alanyl tRNA synthetase second additional domain | IPR012947 | Threonyl/alanyl tRNA synthetase, SAD | 649 | 693 | 3.9E-14 |
| FunFam | G3DSA:2.40.30.130:FF:000001 | Alanine--tRNA ligase | - | - | 448 | 548 | 3.4E-31 |
| PRINTS | PR00980 | Alanyl-tRNA synthetase signature | IPR002318 | Alanine-tRNA ligase, class IIc | 232 | 245 | 2.0E-29 |
| NCBIfam | TIGR00344 | JCVI: alanine--tRNA ligase | IPR002318 | Alanine-tRNA ligase, class IIc | 5 | 851 | 0.0 |
| CDD | cd00673 | AlaRS_core | - | - | 3 | 247 | 0.0 |
| FunFam | G3DSA:3.10.310.40:FF:000001 | Alanine--tRNA ligase | - | - | 761 | 868 | 4.7E-29 |
| Pfam | PF02272 | DHHA1 domain | IPR003156 | DHHA1 domain | 731 | 862 | 6.7E-26 |
| PRINTS | PR00980 | Alanyl-tRNA synthetase signature | IPR002318 | Alanine-tRNA ligase, class IIc | 278 | 294 | 2.0E-29 |
| PRINTS | PR00980 | Alanyl-tRNA synthetase signature | IPR002318 | Alanine-tRNA ligase, class IIc | 205 | 216 | 2.0E-29 |
Search for additional functional domains at the NCBI CDD database website. Go to this protein's amino acid sequence and follow the link.