Pseudomonas putida GB-1, PputGB1_3979 (alaS)
Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
Gene Ontology
| Ontology | Accession | Term | GO Evidence | Evidence Ontology (ECO) Code | Reference | Comments |
|---|---|---|---|---|---|---|
| Cellular Component | GO:0005737 | cytoplasm |
Inferred from Sequence Model
Term mapped from: InterPro:SSF101353
|
ECO:0000259 match to InterPro signature evidence used in automatic assertion |
||
| Molecular Function | GO:0003676 | nucleic acid binding |
Inferred from Sequence Model
Term mapped from: InterPro:PF02272
|
ECO:0000259 match to InterPro signature evidence used in automatic assertion |
||
| Molecular Function | GO:0004813 | alanine-tRNA ligase activity |
Inferred from Sequence Model
Term mapped from: InterPro:PF01411
|
ECO:0000259 match to InterPro signature evidence used in automatic assertion |
||
| Molecular Function | GO:0004812 | aminoacyl-tRNA ligase activity |
Inferred from Sequence Model
Term mapped from: InterPro:SM00863
|
ECO:0000259 match to InterPro signature evidence used in automatic assertion |
||
| Biological Process | GO:0043039 | tRNA aminoacylation |
Inferred from Sequence Model
Term mapped from: InterPro:SM00863
|
ECO:0000259 match to InterPro signature evidence used in automatic assertion |
||
| Biological Process | GO:0006419 | alanyl-tRNA aminoacylation |
Inferred from Sequence Model
Term mapped from: InterPro:PF01411
|
ECO:0000259 match to InterPro signature evidence used in automatic assertion |
||
| Molecular Function | GO:0005524 | ATP binding |
Inferred from Sequence Model
Term mapped from: InterPro:PF01411
|
ECO:0000259 match to InterPro signature evidence used in automatic assertion |
||
| Molecular Function | GO:0000166 | nucleotide binding |
Inferred from Sequence Model
Term mapped from: InterPro:PF01411
|
ECO:0000259 match to InterPro signature evidence used in automatic assertion |
Functional Classifications Manually Assigned by PseudoCAP
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|
Pathways
| Database | Xref | Pathway | Version | Evidence | PMID |
|---|---|---|---|---|---|
| KEGG | ppg00970 | Aminoacyl-tRNA biosynthesis | 81.0+/01-23, Jan 17 |
ECO:0000249
sequence similarity evidence used in automatic assertion |
Functional Predictions from Interpro
| Analysis | Accession | Description | Interpro Accession | Interpro Description | Amino Acid Start | Amino Acid Stop | E-value |
|---|---|---|---|---|---|---|---|
| Pfam | PF02272 | DHHA1 domain | IPR003156 | DHHA1 domain | 756 | 889 | 2.6E-25 |
| Coils | Coil | Coil | - | - | 729 | 781 | - |
| Pfam | PF07973 | Threonyl and Alanyl tRNA synthetase second additional domain | IPR012947 | Threonyl/alanyl tRNA synthetase, SAD | 674 | 718 | 2.3E-15 |
| PRINTS | PR00980 | Alanyl-tRNA synthetase signature | IPR002318 | Alanine-tRNA ligase, class IIc | 230 | 241 | 1.3E-30 |
| Gene3D | G3DSA:2.40.30.130 | - | - | - | 469 | 573 | 3.1E-17 |
| PRINTS | PR00980 | Alanyl-tRNA synthetase signature | IPR002318 | Alanine-tRNA ligase, class IIc | 327 | 340 | 1.3E-30 |
| CDD | cd00673 | AlaRS_core | - | - | 26 | 272 | 0.0 |
| PANTHER | PTHR11777 | ALANYL-TRNA SYNTHETASE | - | - | 19 | 893 | 0.0 |
| PRINTS | PR00980 | Alanyl-tRNA synthetase signature | IPR002318 | Alanine-tRNA ligase, class IIc | 257 | 270 | 1.3E-30 |
| PRINTS | PR00980 | Alanyl-tRNA synthetase signature | IPR002318 | Alanine-tRNA ligase, class IIc | 97 | 108 | 1.3E-30 |
| SUPERFAMILY | SSF55681 | Class II aaRS and biotin synthetases | IPR045864 | Class II Aminoacyl-tRNA synthetase/Biotinyl protein ligase (BPL) and lipoyl protein ligase (LPL) | 24 | 276 | 4.97E-106 |
| Gene3D | G3DSA:3.30.930.10 | Bira Bifunctional Protein; Domain 2 | IPR045864 | Class II Aminoacyl-tRNA synthetase/Biotinyl protein ligase (BPL) and lipoyl protein ligase (LPL) | 24 | 273 | 5.2E-122 |
| SUPERFAMILY | SSF50447 | Translation proteins | IPR009000 | Translation protein, beta-barrel domain superfamily | 476 | 573 | 3.53E-20 |
| Gene3D | G3DSA:3.30.54.20 | - | - | - | 645 | 699 | 2.5E-55 |
| FunFam | G3DSA:3.30.980.10:FF:000004 | Alanine--tRNA ligase, cytoplasmic | - | - | 574 | 733 | 2.5E-39 |
| FunFam | G3DSA:2.40.30.130:FF:000001 | Alanine--tRNA ligase | - | - | 473 | 573 | 3.2E-33 |
| SUPERFAMILY | SSF101353 | Putative anticodon-binding domain of alanyl-tRNA synthetase (AlaRS) | IPR018162 | Alanine-tRNA ligase, class IIc, anti-codon-binding domain superfamily | 277 | 486 | 2.35E-62 |
| Coils | Coil | Coil | - | - | 389 | 409 | - |
| NCBIfam | TIGR00344 | JCVI: alanine--tRNA ligase | IPR002318 | Alanine-tRNA ligase, class IIc | 28 | 876 | 0.0 |
| PRINTS | PR00980 | Alanyl-tRNA synthetase signature | IPR002318 | Alanine-tRNA ligase, class IIc | 303 | 319 | 1.3E-30 |
| Hamap | MF_00036_B | Alanine--tRNA ligase [alaS]. | IPR023033 | Alanine-tRNA ligase, eukaryota/bacteria | 1 | 897 | 200.886063 |
| Coils | Coil | Coil | - | - | 630 | 650 | - |
| FunFam | G3DSA:3.10.310.40:FF:000001 | Alanine--tRNA ligase | - | - | 786 | 892 | 1.9E-28 |
| Pfam | PF01411 | tRNA synthetases class II (A) | IPR018164 | Alanyl-tRNA synthetase, class IIc, N-terminal | 28 | 576 | 0.0 |
| SMART | SM00863 | tRNA_SAD_4 | IPR012947 | Threonyl/alanyl tRNA synthetase, SAD | 674 | 718 | 1.1E-18 |
| SUPERFAMILY | SSF55186 | ThrRS/AlaRS common domain | IPR018163 | Threonyl/alanyl tRNA synthetase, class II-like, putative editing domain superfamily | 573 | 730 | 1.7E-48 |
| FunFam | G3DSA:3.30.54.20:FF:000001 | Alanine--tRNA ligase | - | - | 645 | 699 | 1.2E-25 |
| Gene3D | G3DSA:3.30.980.10 | - | - | - | 584 | 720 | 2.5E-55 |
| Gene3D | G3DSA:3.10.310.40 | - | - | - | 786 | 892 | 4.2E-32 |
| FunFam | G3DSA:3.30.930.10:FF:000004 | Alanine--tRNA ligase | - | - | 23 | 273 | 5.0E-127 |
| Gene3D | G3DSA:6.10.250.550 | - | - | - | 722 | 784 | 1.7E-6 |
Search for additional functional domains at the NCBI CDD database website. Go to this protein's amino acid sequence and follow the link.