Pseudomonas mendocina NK-01, MDS_3757

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown

Gene Ontology

Ontology Accession Term GO Evidence Evidence Ontology (ECO) Code Reference Comments
Biological Process GO:0009098 leucine biosynthetic process
ISM
Inferred from Sequence Model
Term mapped from: InterPro:MF_00572
ECO:0000259
match to InterPro signature evidence used in automatic assertion
Molecular Function GO:0003852 2-isopropylmalate synthase activity
ISM
Inferred from Sequence Model
Term mapped from: InterPro:MF_00572
ECO:0000259
match to InterPro signature evidence used in automatic assertion
Molecular Function GO:0003824 catalytic activity
ISM
Inferred from Sequence Model
Term mapped from: InterPro:PF00682
ECO:0000259
match to InterPro signature evidence used in automatic assertion

Functional Classifications Manually Assigned by PseudoCAP

Pathways

Database Xref Pathway Version Evidence PMID
KEGG pmk00620 Pyruvate metabolism 81.0+/01-23, Jan 17 ECO:0000249
sequence similarity evidence used in automatic assertion
KEGG pmk01210 2-Oxocarboxylic acid metabolism 81.0+/01-23, Jan 17 ECO:0000249
sequence similarity evidence used in automatic assertion
KEGG pmk00290 Valine, leucine and isoleucine biosynthesis 81.0+/01-23, Jan 17 ECO:0000249
sequence similarity evidence used in automatic assertion
KEGG pmk01110 Biosynthesis of secondary metabolites 81.0+/01-23, Jan 17 ECO:0000249
sequence similarity evidence used in automatic assertion
MetaCyc 3-methylbutanol biosynthesis (engineered) InterPro 5.36-75.0 ECO:0000259
match to InterPro signature evidence used in automatic assertion
KEGG pmk01230 Biosynthesis of amino acids 81.0+/01-23, Jan 17 ECO:0000249
sequence similarity evidence used in automatic assertion
KEGG pmk01100 Metabolic pathways 81.0+/01-23, Jan 17 ECO:0000249
sequence similarity evidence used in automatic assertion

Functional Predictions from Interpro

Analysis Accession Description Interpro Accession Interpro Description Amino Acid Start Amino Acid Stop E-value
Coils Coil Coil - - 545 556 -
Pfam PF08502 LeuA allosteric (dimerisation) domain IPR013709 2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain 422 548 1.6E-28
Gene3D G3DSA:3.20.20.70 Aldolase class I IPR013785 Aldolase-type TIM barrel 12 376 0.0
NCBIfam TIGR00970 JCVI: 2-isopropylmalate synthase IPR005668 2-isopropylmalate synthase 7 547 0.0
SUPERFAMILY SSF89000 post-HMGL domain-like - - 388 438 1.22E-13
SUPERFAMILY SSF110921 2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain IPR036230 2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain superfamily 448 547 1.57E-31
PANTHER PTHR46911 - - - 2 554 0.0
Gene3D G3DSA:3.30.160.270 - IPR036230 2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain superfamily 377 547 2.7E-55
SMART SM00917 LeuA_dimer_2 IPR013709 2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain 422 548 2.3E-29
Pfam PF00682 HMGL-like IPR000891 Pyruvate carboxyltransferase 33 313 3.3E-80
Hamap MF_00572 2-isopropylmalate synthase [leuA]. IPR005668 2-isopropylmalate synthase 8 549 53.547909
FunFam G3DSA:3.20.20.70:FF:000045 2-isopropylmalate synthase - - 11 376 0.0
CDD cd07942 DRE_TIM_LeuA IPR039371 LeuA, N-terminal catalytic TIM barrel domain 32 315 0.0
SUPERFAMILY SSF51569 Aldolase - - 22 330 1.19E-99

Search for additional functional domains at the NCBI CDD database website. Go to this protein's amino acid sequence and follow the link.