Pseudomonas aeruginosa PAO1, PA0216

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0216
Name
Synonym: madM
Replicon chromosome
Genomic location 243841 - 244605 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
CommentBIOLOG data shows growth on malonate. Annotation assigns PA0215 and PA0216 as having 71% and 81% homology with MadL and MadM from Malonomonas rubra.

Cross-References

RefSeq NP_248907.1
GI 15595413
Affymetrix PA0216_at
Entrez 879529
GenBank AAG03605.1
INSDC AAG03605.1
NCBI Locus Tag PA0216
protein_id(GenBank) gb|AAG03605.1|AE004459_9|gnl|PseudoCAP|PA0216
TIGR NTL03PA00217
UniParc UPI00000C4F9F
UniProtKB Acc Q9I6S2
UniProtKB ID Q9I6S2_PSEAE
UniRef100 UniRef100_W0YMD6
UniRef50 UniRef50_Q87V62
UniRef90 UniRef90_A6UY11

Product

Feature Type CDS
Coding Frame 1
Product Name
malonate transporter MadM
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Charge (pH 7) 4.16
Kyte-Doolittle Hydrophobicity Value 1.045
Molecular Weight (kDa) 25.4
Isoelectric Point (pI) 9.32

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 30 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000215 (475 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0216

Human Homologs

References

Identification of an Na+-dependent malonate transporter of Malonomonas rubra and its dependence on two separate genes.
Schaffitzel C, Berg M, Dimroth P, Pos KM
J Bacteriol 1998 May;180(10):2689-93
PubMed ID: 9573154