Pseudomonas aeruginosa PAO1, PA0500 (bioB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA0500
Name
bioB
Replicon chromosome
Genomic location 559644 - 560702 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249191.1
GI 15595697
Affymetrix PA0500_bioB_at
DNASU PaCD00006210
Entrez 877668
GenBank AAG03889.1
INSDC AAG03889.1
NCBI Locus Tag PA0500
protein_id(GenBank) gb|AAG03889.1|AE004487_3|gnl|PseudoCAP|PA0500
TIGR NTL03PA00501
UniParc UPI00000C5090
UniProtKB Acc Q9I618
UniProtKB ID BIOB_PSEAE
UniRef100 UniRef100_B7V485
UniRef50 UniRef50_A9EXH2
UniRef90 UniRef90_A4XZR9

Product

Feature Type CDS
Coding Frame 1
Product Name
biotin synthase
Product Name Confidence: Class 2
Synonyms biotin synthetase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -5.77
Kyte-Doolittle Hydrophobicity Value -0.315
Molecular Weight (kDa) 39.1
Isoelectric Point (pI) 6.01

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold Protein Structure Database

Available for P. aeruginosa PAO1.

View predicted 3D structure in AlphaFold DB

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 475 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000487 (554 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0500
Search term: bioB
Search term: biotin synthase

Human Homologs

References

Biotin synthase: purification, characterization as a [2Fe-2S]cluster protein, and in vitro activity of the Escherichia coli bioB gene product.
Sanyal I, Cohen G, Flint DH
Biochemistry 1994 Mar;33(12):3625-31
PubMed ID: 8142361
Biotin synthase from Escherichia coli: isolation of an enzyme-generated intermediate and stoichiometry of S-adenosylmethionine use.
Shaw NM, Birch OM, Tinschert A, Venetz V, Dietrich R, Savoy LA
Biochem. J. 1998 Mar;330 ( Pt 3)
PubMed ID: 9494071
Transfer of sulfur to biotin from biotin synthase (BioB protein).
Gibson KJ, Pelletier DA, Turner IM
Biochem. Biophys. Res. Commun. 1999 Jan;254(3):632-5
PubMed ID: 9920791