Pseudomonas aeruginosa PAO1, PA0509 (nirN)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Replicon chromosome
Genomic location 571111 - 572592 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs


RefSeq NP_249200.1
GI 15595706
Affymetrix PA0509_nirN_at
Entrez 878428
GenBank AAG03898.1
INSDC AAG03898.1
NCBI Locus Tag PA0509
protein_id(GenBank) gb|AAG03898.1|AE004488_2|gnl|PseudoCAP|PA0509
UniParc UPI00000C5098
UniProtKB Acc Q9I609
UniRef100 UniRef100_W0YNA8
UniRef50 UniRef50_F8GTF5
UniRef90 UniRef90_A6UYX0


Feature Type CDS
Coding Frame 1
Product Name
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) 2.79
Kyte-Doolittle Hydrophobicity Value -0.203
Molecular Weight (kDa) 54.0
Isoelectric Point (pI) 7.54

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown (This protein may have multiple localization sites) Class 3
Unknown (This protein may have multiple localization sites) Class 3
Periplasmic Class 1
Additional evidence for subcellular localization

AlphaFold Protein Structure Database

Available for P. aeruginosa PAO1.

View predicted 3D structure in AlphaFold DB

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6RTE OXIDOREDUCTASE 05/23/19 Dihydro-heme d1 dehydrogenase NirN in complex with DHE Pseudomonas aeruginosa 1.94 X-RAY DIFFRACTION 99.8
6RTD OXIDOREDUCTASE 05/23/19 Dihydro-heme d1 dehydrogenase NirN in complex with DHE Pseudomonas aeruginosa 2.36 X-RAY DIFFRACTION 99.8

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 57 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000494 (728 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA0509
Search term: nirN
Search term: NirN

Human Homologs


Two c-type cytochromes, NirM and NirC, encoded in the nir gene cluster of Pseudomonas aeruginosa act as electron donors for nitrite reductase.
Hasegawa N, Arai H, Igarashi Y
Biochem Biophys Res Commun 2001 Nov 16;288(5):1223-30
PubMed ID: 11700043
Gene cluster for dissimilatory nitrite reductase (nir) from Pseudomonas aeruginosa: sequencing and identification of a locus for heme d1 biosynthesis.
Kawasaki S, Arai H, Kodama T, Igarashi Y
J. Bacteriol. 1997 Jan;179(1):235-42
PubMed ID: 8982003
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
NirN protein from Pseudomonas aeruginosa is a novel electron-bifurcating dehydrogenase catalyzing the last step of heme d1 biosynthesis.
Adamczack J, Hoffmann M, Papke U, Haufschildt K, Nicke T, Bröring M, Sezer M, Weimar R, Kuhlmann U, Hildebrandt P, Layer G
J. Biol. Chem. 2014 Oct;289(44):30753-62
PubMed ID: 25204657