Pseudomonas aeruginosa PAO1, PA0575 (rmcA)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
Locus Tag
Replicon chromosome
Genomic location 630527 - 634264 (- strand)
Transposon Mutants 9 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs


RefSeq NP_249266.1
GI 15595772
Affymetrix PA0575_at
Entrez 882126
GenBank AAG03964.1
INSDC AAG03964.1
NCBI Locus Tag PA0575
protein_id(GenBank) gb|AAG03964.1|AE004493_9|gnl|PseudoCAP|PA0575
UniParc UPI00000C50C3
UniProtKB Acc Q9I5W1
UniRef100 UniRef100_Q9I5W1
UniRef50 UniRef50_Q4K4W0
UniRef90 UniRef90_Q9I5W1


Feature Type CDS
Coding Frame 1
Product Name
redox regulator of c_di_GMP, RmcA
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -30.54
Kyte-Doolittle Hydrophobicity Value -0.178
Molecular Weight (kDa) 139.7
Isoelectric Point (pI) 5.45

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7EJG PEPTIDE BINDING PROTEIN 04/02/21 Crystal structure of PBP domain of RMCA Pseudomonas aeruginosa 1.68 X-RAY DIFFRACTION 96.2
5M3C HYDROLASE 10/14/16 Structure of the hybrid domain (GGDEF-EAL) of PA0575 from Pseudomonas aeruginosa PAO1 at 2.8 Ang. with GTP and Ca2+ bound to the active site of the GGDEF domain Pseudomonas aeruginosa 2.8 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 564 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000558 (491 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA0575
Search term: rmcA

Human Homologs


PAS domains: internal sensors of oxygen, redox potential, and light.
Taylor BL, Zhulin IB
Microbiol Mol Biol Rev 1999 Jun;63(2):479-506
PubMed ID: 10357859
Insights into the GTP-dependent allosteric control of c-di-GMP hydrolysis from the crystal structure of PA0575 protein from Pseudomonas aeruginosa.
Mantoni F, Paiardini A, Brunotti P, D'Angelo C, Cervoni L, Paone A, Cappellacci L, Petrelli R, Ricciutelli M, Leoni L, Rampioni G, Arcovito A, Rinaldo S, Cutruzzolà F, Giardina G
FEBS J 2018 Oct;285(20):3815-3834
PubMed ID: 30106221
Electron-shuttling antibiotics structure bacterial communities by modulating cellular levels of c-di-GMP.
Okegbe C, Fields BL, Cole SJ, Beierschmitt C, Morgan CJ, Price-Whelan A, Stewart RC, Lee VT, Dietrich LEP
Proc Natl Acad Sci U S A 2017 Jun 27;114(26):E5236-E5245
PubMed ID: 28607054
PA0575 (RmcA) interacts with other c-di-GMP metabolizing proteins in Pseudomonas aeruginosa PAO1.
Yao Y, Xi N, Hai E, Zhang X, Guo J, Lin Z, Huang W
J Gen Appl Microbiol 2023 Jan 24;68(5):232-241
PubMed ID: 35732459