Pseudomonas aeruginosa PAO1, PA1004 (nadA)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
Locus Tag
Replicon chromosome
Genomic location 1087843 - 1088901 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentReaction not utilizing O2 is necessary to produce spermidine in anaerobic conditions, as shown by gap analysis and explained in references.


RefSeq NP_249695.1
GI 15596201
Affymetrix PA1004_nadA_at
Entrez 883095
GenBank AAG04393.1
INSDC AAG04393.1
NCBI Locus Tag PA1004
protein_id(GenBank) gb|AAG04393.1|AE004533_4|gnl|PseudoCAP|PA1004
UniParc UPI000012FC67
UniProtKB Acc Q9I4W9
UniRef100 UniRef100_Q02IG9
UniRef50 UniRef50_Q8EEN5
UniRef90 UniRef90_Q02IG9


Feature Type CDS
Coding Frame 1
Product Name
quinolinate synthetase A
Product Name Confidence: Class 2
Synonyms L-aspartate oxidase (aerobic/anaerobic) NadA
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -9.71
Kyte-Doolittle Hydrophobicity Value -0.037
Molecular Weight (kDa) 38.4
Isoelectric Point (pI) 5.40

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 2
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 517 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000967 (536 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA1004
Search term: nadA

Human Homologs


Molecular biology of pyridine nucleotide biosynthesis in Escherichia coli. Cloning and characterization of quinolinate synthesis genes nadA and nadB.
Flachmann R, Kunz N, Seifert J, Gütlich M, Wientjes FJ, Läufer A, Gassen HG
Eur J Biochem 1988 Aug 1;175(2):221-8
PubMed ID: 2841129
A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.
Oshima T, Aiba H, Baba T, Fujita K, Hayashi K, Honjo A, Ikemoto K, Inada T, Itoh T, Kajihara M, Kanai K, Kashimoto K, Kimura S, Kitagawa M, Makino K, Masuda S, Miki T, Mizobuchi K, Mori H, Motomura K, Nakamura Y, Nashimoto H, Nishio Y, Saito N, Horiuchi T
DNA Res 1996 Jun 30;3(3):137-55
PubMed ID: 8905232
Genetic linkage in Pseudomonas aeruginosa of algT and nadB: mutation in nadB does not affect NAD biosynthesis or alginate production.
DeVries CA, Hassett DJ, Flynn JL, Ohman DE
Gene 1995 Apr 14;156(1):63-7
PubMed ID: 7737518