Pseudomonas aeruginosa PAO1, PA1084 (flgI)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1084
Name
flgI
Replicon chromosome
Genomic location 1169822 - 1170931 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249775.1
GI 15596281
Affymetrix PA1084_flgI_at
DNASU PaCD00006501
Entrez 878536
GenBank AAG04473.1
INSDC AAG04473.1
NCBI Locus Tag PA1084
protein_id(GenBank) gb|AAG04473.1|AE004539_15|gnl|PseudoCAP|PA1084
TIGR NTL03PA01085
UniParc UPI000012A932
UniProtKB Acc Q9I4P5
UniProtKB ID FLGI_PSEAE
UniRef100 UniRef100_A6V9B1
UniRef50 UniRef50_P58203
UniRef90 UniRef90_A6V9B1

Product

Feature Type CDS
Coding Frame 1
Product Name
flagellar P-ring protein precursor FlgI
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) 0.96
Kyte-Doolittle Hydrophobicity Value 0.129
Molecular Weight (kDa) 38.2
Isoelectric Point (pI) 7.84

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Periplasmic Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database VFDB : VF0273
Category Flagella
Evidence
Host Organism
Notes
Reference 14617143

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 243 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001046 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1084
Search term: flgI

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
The flaFIX gene product of Salmonella typhimurium is a flagellar basal body component with a signal peptide for export.
Homma M, Komeda Y, Iino T, Macnab RM
J Bacteriol 1987 Apr;169(4):1493-8
PubMed ID: 3549691
L-, P-, and M-ring proteins of the flagellar basal body of Salmonella typhimurium: gene sequences and deduced protein sequences.
Jones CJ, Homma M, Macnab RM
J Bacteriol 1989 Jul;171(7):3890-900
PubMed ID: 2544561
Molecular cloning of two Pseudomonas flagellin genes and basal body structural genes.
Winstanley C, Morgan JA, Pickup RW, Saunders JR
Microbiology (Reading) 1994 Aug;140 ( Pt 8):2019-31
PubMed ID: 7921252
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994