Pseudomonas aeruginosa PAO1, PA1662 (clpV2)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1662
Name
clpV2
Replicon chromosome
Genomic location 1810751 - 1813384 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs

Cross-References

RefSeq NP_250353.1
GI 15596859
Affymetrix PA1662_at
Entrez 878010
GenBank AAG05051.1
NCBI Locus Tag PA1662
protein_id(GenBank) gb|AAG05051.1|AE004594_3|gnl|PseudoCAP|PA1662
TIGR NTL03PA01663
UniProtKB Acc Q9I363
UniProtKB ID Q9I363_PSEAE

Product

Feature Type CDS
Coding Frame 1
Product Name
clpV2
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -16.79
Kyte-Doolittle Hydrophobicity Value -0.201
Molecular Weight (kDa) 95.8
Isoelectric Point (pI) 5.47

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database PseudoCAP
Category Hcp secretion island-2 encoded type VI secretion system (H2-T6SS)
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Caenorhabditis elegans
Notes clpV2 mutant significantly less virulent than WT PAO1 strain in C elegans slow killing assay
Reference 22665491
Source 2
Database PseudoCAP
Category Hcp secretion island-2 encoded type VI secretion system (H2-T6SS)
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Homo sapiens
Notes Infection of Calu-3 cells with PAO1 clpV2 mutant resulted in 50% decrease in Akt phosphorylation levels
Reference 22665491

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 597 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000089 (1168 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1662
Search term: clpV2
Search term: clpV2

Human Homologs

References

The heat shock protein ClpB mediates the development of thermotolerance in the cyanobacterium Synechococcus sp. strain PCC 7942.
Eriksson MJ, Clarke AK
J Bacteriol 1996 Aug;178(16):4839-46
PubMed ID: 8759846