Pseudomonas aeruginosa PAO1, PA2363 (hsiJ3)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA2363
Name
hsiJ3
Replicon chromosome
Genomic location 2612817 - 2614148 (- strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_251053.1
GI 15597559
Affymetrix PA2363_at
Entrez 882215
GenBank AAG05751.1
INSDC AAG05751.1
NCBI Locus Tag PA2363
protein_id(GenBank) gb|AAG05751.1|AE004662_6|gnl|PseudoCAP|PA2363
TIGR NTL03PA02363
UniParc UPI00000C568F
UniProtKB Acc Q9I1B6
UniProtKB ID Q9I1B6_PSEAE
UniRef100 UniRef100_Q9I1B6
UniRef50 UniRef50_G8QBI1
UniRef90 UniRef90_W0YSS6

Product

Feature Type CDS
Coding Frame 1
Product Name
HsiJ3
Product Name Confidence: Class 1
Synonyms hypothetical protein
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -8.48
Kyte-Doolittle Hydrophobicity Value 0.077
Molecular Weight (kDa) 47.8
Isoelectric Point (pI) 5.16

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Virulence Evidence

Source 1
Database PseudoCAP
Category Hcp secretion island-3 encoded type VI secretion system (H3-T6SS)
Evidence ECO:0000317
genomic context evidence used in manual assertion
Host Organism Caenorhabditis elegans
Notes
Infection model Caenorhabditis elegans slow killing assay. H3-T6SS rendered inactive via clpV3 deletion.
Reference 24204589

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 70 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003157 (332 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2363
Search term: hsiJ3
Search term: HsiJ3

Human Homologs

References

A virulence locus of Pseudomonas aeruginosa encodes a protein secretion apparatus.
Mougous JD, Cuff ME, Raunser S, Shen A, Zhou M, Gifford CA, Goodman AL, Joachimiak G, Ordoñez CL, Lory S, Walz T, Joachimiak A, Mekalanos JJ
Science 2006 Jun;312(5779):1526-30
PubMed ID: 16763151
Divergent control of two type VI secretion systems by RpoN in Pseudomonas aeruginosa.
Sana TG, Soscia C, Tonglet CM, Garvis S, Bleves S
PLoS ONE 2013 ;8(10):e76030
PubMed ID: 24204589