Pseudomonas aeruginosa PAO1, PA2611 (cysG)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2611
Name
cysG
Replicon chromosome
Genomic location 2952017 - 2953414 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_251301.1
GI 15597807
Affymetrix PA2611_cysG_at
Entrez 882317
GenBank AAG05999.1
INSDC AAG05999.1
NCBI Locus Tag PA2611
protein_id(GenBank) gb|AAG05999.1|AE004689_11|gnl|PseudoCAP|PA2611
TIGR NTL03PA02611
UniParc UPI00000C5768
UniProtKB Acc Q9I0M7
UniProtKB ID CYSG_PSEAE
UniRef100 UniRef100_Q9I0M7
UniRef50 UniRef50_P25924
UniRef90 UniRef90_Q9I0M7

Product

Feature Type CDS
Coding Frame 1
Product Name
siroheme synthase
Product Name Confidence: Class 2
Synonyms uroporphyrinogen III methylase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -5.76
Kyte-Doolittle Hydrophobicity Value 0.053
Molecular Weight (kDa) 50.4
Isoelectric Point (pI) 6.28

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 537 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002940 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2611
Search term: cysG
Search term: siroheme synthase

Human Homologs

References

The Escherichia coli cysG gene encodes S-adenosylmethionine-dependent uroporphyrinogen III methylase.
Warren MJ, Roessner CA, Santander PJ, Scott AI
Biochem J 1990 Feb 1;265(3):725-9
PubMed ID: 2407234
The Escherichia coli cysG gene encodes the multifunctional protein, siroheme synthase.
Spencer JB, Stolowich NJ, Roessner CA, Scott AI
FEBS Lett 1993 Nov 29;335(1):57-60
PubMed ID: 8243665