Pseudomonas aeruginosa PAO1, PA2853 (oprI)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
Locus Tag
Replicon chromosome
Genomic location 3206915 - 3207166 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1


RefSeq NP_251543.1
GI 15598049
Affymetrix PA2853_oprI_at
DNASU PaCD00007242
Entrez 879851
GenBank AAG06241.1
INSDC AAG06241.1
NCBI Locus Tag PA2853
protein_id(GenBank) gb|AAG06241.1|AE004712_1|gnl|PseudoCAP|PA2853
UniParc UPI000002C273
UniProtKB Acc P11221
UniRef100 UniRef100_P11221
UniRef50 UniRef50_P11221
UniRef90 UniRef90_P11221


Feature Type CDS
Coding Frame 1
Product Name
Outer membrane lipoprotein OprI precursor
Product Name Confidence: Class 1
Evidence for Translation
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (8.83 kDa, PMID:21751344)
Charge (pH 7) 1.20
Kyte-Doolittle Hydrophobicity Value -0.541
Molecular Weight (kDa) 8.8
Isoelectric Point (pI) 8.51

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Outer Membrane Class 1
Outer Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 2 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG005132 (452 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA2853
Search term: oprI

Human Homologs


Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Cloning and analysis of the gene for the major outer membrane lipoprotein from Pseudomonas aeruginosa.
Cornelis P, Bouia A, Belarbi A, Guyonvarch A, Kammerer B, Hannaert V, Hubert JC
Mol Microbiol 1989 Mar;3(3):421-8
PubMed ID: 2473376
Pseudomonas aeruginosa outer membrane lipoprotein I gene: molecular cloning, sequence, and expression in Escherichia coli.
Duchêne M, Barron C, Schweizer A, von Specht BU, Domdey H
J Bacteriol 1989 Aug;171(8):4130-7
PubMed ID: 2502533
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344