Pseudomonas aeruginosa PAO1, PA2856 (tesA)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
Locus Tag
Synonym: apeA
Replicon chromosome
Genomic location 3208673 - 3209278 (- strand)
Transposon Mutants 3 transposon mutants in PAO1


RefSeq NP_251546.1
GI 15598052
Affymetrix PA2856_tesA_at
DNASU PaCD00007244
Entrez 878554
GenBank AAG06244.1
INSDC AAG06244.1
NCBI Locus Tag PA2856
protein_id(GenBank) gb|AAG06244.1|AE004712_4|gnl|PseudoCAP|PA2856
UniParc UPI00000C5847
UniProtKB Acc Q9HZY8
UniRef100 UniRef100_Q9HZY8
UniRef50 UniRef50_Q9HZY8
UniRef90 UniRef90_Q9HZY8


Feature Type CDS
Coding Frame 1
Product Name
lysophospholipase A
Product Name Confidence: Class 1
Synonyms lysophospholipase L1
lecithinase B
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -0.80
Kyte-Doolittle Hydrophobicity Value 0.318
Molecular Weight (kDa) 21.0
Isoelectric Point (pI) 5.91

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 2
Periplasmic Class 1
Unknown (This protein may have multiple localization sites) Class 3
Unknown (This protein may have multiple localization sites) Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4JGG HYDROLASE 03/01/13 Crystal Structure of TesA Pseudomonas aeruginosa 1.9 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 245 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002713 (533 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA2856
Search term: tesA

Human Homologs


Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Molecular cloning, sequencing, and mapping of the gene encoding protease I and characterization of proteinase and proteinase-defective Escherichia coli mutants.
Ichihara S, Matsubara Y, Kato C, Akasaka K, Mizushima S
J Bacteriol 1993 Feb;175(4):1032-7
PubMed ID: 8432696
Escherichia coli thioesterase I, molecular cloning and sequencing of the structural gene and identification as a periplasmic enzyme.
Cho H, Cronan JE Jr
J Biol Chem 1993 May 5;268(13):9238-45
PubMed ID: 8098033
Structural and functional characterisation of TesA - a novel lysophospholipase A from Pseudomonas aeruginosa.
Kovačić F, Granzin J, Wilhelm S, Kojić-Prodić B, Batra-Safferling R, Jaeger KE
PLoS ONE 2013 ;8(7):e69125
PubMed ID: 23874889