Pseudomonas aeruginosa PAO1, PA2961 (holB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2961
Name
holB
Replicon chromosome
Genomic location 3320064 - 3321050 (- strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_251651.1
GI 15598157
Affymetrix PA2961_holB_at
DNASU PaCD00007292
Entrez 880379
GenBank AAG06349.1
INSDC AAG06349.1
NCBI Locus Tag PA2961
protein_id(GenBank) gb|AAG06349.1|AE004722_5|gnl|PseudoCAP|PA2961
TIGR NTL03PA02961
UniParc UPI000012CAC8
UniProtKB Acc P52024
UniProtKB ID HOLB_PSEAE
UniRef100 UniRef100_P52024
UniRef50 UniRef50_P52024
UniRef90 UniRef90_P52024

Product

Feature Type CDS
Coding Frame 1
Product Name
DNA polymerase III, delta prime subunit
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -1.53
Kyte-Doolittle Hydrophobicity Value -0.027
Molecular Weight (kDa) 35.7
Isoelectric Point (pI) 6.62

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 587 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002621 (534 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2961
Search term: holB

Human Homologs

References

Identification, isolation, and characterization of the structural gene encoding the delta' subunit of Escherichia coli DNA polymerase III holoenzyme.
Carter JR, Franden MA, Aebersold R, McHenry CS
J Bacteriol 1993 Jun;175(12):3812-22
PubMed ID: 8509334
Identification of a novel gene, pilZ, essential for type 4 fimbrial biogenesis in Pseudomonas aeruginosa.
Alm RA, Bodero AJ, Free PD, Mattick JS
J Bacteriol 1996 Jan;178(1):46-53
PubMed ID: 8550441