Pseudomonas aeruginosa PAO1, PA3186 (oprB)

Cytoplasmic Membrane
Outer Membrane
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Replicon chromosome
Genomic location 3575912 - 3577276 (- strand)
Transposon Mutants 5 transposon mutants in PAO1
Transposon Mutants in orthologs 4 transposon mutants in orthologs
CommentLocalication to outer membrane vesicle confirmed in three separate studies (PMID:22909304, PMID:21751344 and PMID:26303878).


RefSeq NP_251876.1
GI 15598382
Affymetrix PA3186_oprB_s_at
Entrez 882788
GenBank AAG06574.1
INSDC AAG06574.1
NCBI Locus Tag PA3186
protein_id(GenBank) gb|AAG06574.1|AE004742_10|gnl|PseudoCAP|PA3186
UniParc UPI0000131F64
UniProtKB Acc Q51485
UniRef100 UniRef100_Q51485
UniRef50 UniRef50_Q51485
UniRef90 UniRef90_Q51485


Feature Type CDS
Coding Frame 1
Product Name
Glucose/carbohydrate outer membrane porin OprB precursor
Product Name Confidence: Class 1
Synonyms D1
carbohydrate-selective porin OprB
Evidence for Translation
Detected using LC-MS/MS (50.75 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -7.16
Kyte-Doolittle Hydrophobicity Value -0.537
Molecular Weight (kDa) 50.8
Isoelectric Point (pI) 5.48

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Class 1
Outer Membrane Vesicle Class 1
Outer Membrane Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 51 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002430 (722 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA3186
Search term: oprB


Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Cloning and nucleotide sequence of the Pseudomonas aeruginosa glucose-selective OprB porin gene and distribution of OprB within the family Pseudomonadaceae.
Wylie JL, Worobec EA
Eur. J. Biochem. 1994 Mar;220(2):505-12
PubMed ID: 8125108
The OprB porin plays a central role in carbohydrate uptake in Pseudomonas aeruginosa.
Wylie JL, Worobec EA
J. Bacteriol. 1995 Jun;177(11):3021-6
PubMed ID: 7768797
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
Identification of proteins associated with the Pseudomonas aeruginosa biofilm extracellular matrix.
Toyofuku M, Roschitzki B, Riedel K, Eberl L
J Proteome Res 2012 Oct 5;11(10):4906-15
PubMed ID: 22909304
Proteome Profiles of Outer Membrane Vesicles and Extracellular Matrix of Pseudomonas aeruginosa Biofilms.
Couto N, Schooling SR, Dutcher JR, Barber J
J Proteome Res 2015 Oct 2;14(10):4207-22
PubMed ID: 26303878