Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA3397
|
Name |
fprA
Synonym: fpr |
Replicon | chromosome |
Genomic location | 3802567 - 3803343 (- strand) |
RefSeq | NP_252087.1 |
GI | 15598593 |
Affymetrix | PA3397_fpr_at |
Entrez | 879913 |
GenBank | AAG06785.1 |
INSDC | AAG06785.1 |
NCBI Locus Tag | PA3397 |
protein_id(GenBank) | gb|AAG06785.1|AE004761_1|gnl|PseudoCAP|PA3397 |
TIGR | NTL03PA03397 |
UniParc | UPI00000C59EC |
UniProtKB Acc | Q9HYK7 |
UniProtKB ID | Q9HYK7_PSEAE |
UniRef100 | UniRef100_W0YU94 |
UniRef50 | UniRef50_Q44532 |
UniRef90 | UniRef90_A0A024HE10 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
FprA
|
Synonyms |
NADP+-dependent ferredoxin reductase FPR NADPH:ferredoxin reductase |
Evidence for Translation |
Verified using two-dimensional gel electrophoresis, MALDI-TOF MS and peptide mass mapping(pI:5.7, molecular weight:29.5, PMID:21674802)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
|
Charge (pH 7) | -5.11 |
Kyte-Doolittle Hydrophobicity Value | -0.346 |
Molecular Weight (kDa) | 29.5 |
Isoelectric Point (pI) | 5.87 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
2QDX | OXIDOREDUCTASE | 06/21/07 | P.Aeruginosa Fpr with FAD | Pseudomonas aeruginosa | 1.55 | X-RAY DIFFRACTION | 100.0 |
3CRZ | OXIDOREDUCTASE | 04/08/08 | Ferredoxin-NADP Reductase | Pseudomonas aeruginosa | 1.9 | X-RAY DIFFRACTION | 100.0 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 282 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG002231 (535 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA3397
Search term: fprA
Search term: FprA
|
Azotobacter vinelandii NADPH:ferredoxin reductase cloning, sequencing, and overexpression.
Isas JM, Yannone SM, Burgess BK
J Biol Chem 1995 Sep 8;270(36):21258-63
PubMed ID: 7673160
|
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
|
Proteomics of the oxidative stress response induced by hydrogen peroxide and paraquat reveals a novel AhpC-like protein in Pseudomonas aeruginosa.
Hare NJ, Scott NE, Shin EH, Connolly AM, Larsen MR, Palmisano G, Cordwell SJ
Proteomics 2011 Aug;11(15):3056-69
PubMed ID: 21674802
|
Biochemical and structural characterization of Pseudomonas aeruginosa Bfd and FPR: ferredoxin NADP+ reductase and not ferredoxin is the redox partner of heme oxygenase under iron-starvation conditions.
Wang A, Zeng Y, Han H, Weeratunga S, Morgan BN, Moënne-Loccoz P, Schönbrunn E, Rivera M
Biochemistry 2007 Oct 30;46(43):12198-211
PubMed ID: 17915950
|
Pseudomonas aeruginosa IscR-Regulated Ferredoxin NADP(+) Reductase Gene (fprB) Functions in Iron-Sulfur Cluster Biogenesis and Multiple Stress Response.
Romsang A, Duang-Nkern J, Wirathorn W, Vattanaviboon P, Mongkolsuk S
PLoS One 2015;10(7):e0134374
PubMed ID: 26230408
|