Pseudomonas aeruginosa PAO1, PA3397 (fprA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3397
Name
fprA
Synonym: fpr
Replicon chromosome
Genomic location 3802567 - 3803343 (- strand)

Cross-References

RefSeq NP_252087.1
GI 15598593
Affymetrix PA3397_fpr_at
Entrez 879913
GenBank AAG06785.1
INSDC AAG06785.1
NCBI Locus Tag PA3397
protein_id(GenBank) gb|AAG06785.1|AE004761_1|gnl|PseudoCAP|PA3397
TIGR NTL03PA03397
UniParc UPI00000C59EC
UniProtKB Acc Q9HYK7
UniProtKB ID Q9HYK7_PSEAE
UniRef100 UniRef100_W0YU94
UniRef50 UniRef50_Q44532
UniRef90 UniRef90_A0A024HE10

Product

Feature Type CDS
Coding Frame 1
Product Name
FprA
Product Name Confidence: Class 1
Synonyms NADP+-dependent ferredoxin reductase
FPR
NADPH:ferredoxin reductase
Evidence for Translation
Verified using two-dimensional gel electrophoresis, MALDI-TOF MS and peptide mass mapping(pI:5.7, molecular weight:29.5, PMID:21674802)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -5.11
Kyte-Doolittle Hydrophobicity Value -0.346
Molecular Weight (kDa) 29.5
Isoelectric Point (pI) 5.87

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
2QDX OXIDOREDUCTASE 06/21/07 P.Aeruginosa Fpr with FAD Pseudomonas aeruginosa 1.55 X-RAY DIFFRACTION 100.0
3CRZ OXIDOREDUCTASE 04/08/08 Ferredoxin-NADP Reductase Pseudomonas aeruginosa 1.9 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 282 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002231 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3397
Search term: fprA
Search term: FprA

Human Homologs

References

Azotobacter vinelandii NADPH:ferredoxin reductase cloning, sequencing, and overexpression.
Isas JM, Yannone SM, Burgess BK
J Biol Chem 1995 Sep 8;270(36):21258-63
PubMed ID: 7673160
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomics of the oxidative stress response induced by hydrogen peroxide and paraquat reveals a novel AhpC-like protein in Pseudomonas aeruginosa.
Hare NJ, Scott NE, Shin EH, Connolly AM, Larsen MR, Palmisano G, Cordwell SJ
Proteomics 2011 Aug;11(15):3056-69
PubMed ID: 21674802
Biochemical and structural characterization of Pseudomonas aeruginosa Bfd and FPR: ferredoxin NADP+ reductase and not ferredoxin is the redox partner of heme oxygenase under iron-starvation conditions.
Wang A, Zeng Y, Han H, Weeratunga S, Morgan BN, Moënne-Loccoz P, Schönbrunn E, Rivera M
Biochemistry 2007 Oct 30;46(43):12198-211
PubMed ID: 17915950
Pseudomonas aeruginosa IscR-Regulated Ferredoxin NADP(+) Reductase Gene (fprB) Functions in Iron-Sulfur Cluster Biogenesis and Multiple Stress Response.
Romsang A, Duang-Nkern J, Wirathorn W, Vattanaviboon P, Mongkolsuk S
PLoS One 2015;10(7):e0134374
PubMed ID: 26230408