Pseudomonas aeruginosa PAO1, PA3520

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3520
Name
Replicon chromosome
Genomic location 3937238 - 3937432 (- strand)
CommentStructural similarity to 5T1P (periplasmic solute-binding protein from Campylobacter jejuni, root-mean-square deviation: 0.4 angstroms). Sequence similarity to 5T1P (97%/68,42%) (GPE). Final predicted ligand: L-alpha-glycerophosphoryl-ethanolamine (PMID: 31631799)

Cross-References

RefSeq NP_252210.1
GI 15598716
Affymetrix PA3520_at
DNASU PaCD00007549
Entrez 878877
GenBank AAG06908.1
INSDC AAG06908.1
NCBI Locus Tag PA3520
protein_id(GenBank) gb|AAG06908.1|AE004772_6|gnl|PseudoCAP|PA3520
TIGR NTL03PA03520
UniParc UPI00000C5A50
UniProtKB Acc Q9HY89
UniProtKB ID Q9HY89_PSEAE
UniRef100 UniRef100_W0YWH1
UniRef50 UniRef50_A6X3Y2
UniRef90 UniRef90_W0YWH1

Product

Feature Type CDS
Coding Frame 1
Product Name
putative periplasmic substrate binding protein
Product Name Confidence: Class 4
Synonyms
Evidence for Translation
Charge (pH 7) -0.36
Kyte-Doolittle Hydrophobicity Value -0.486
Molecular Weight (kDa) 7.1
Isoelectric Point (pI) 6.78

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 1
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 83 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002094 (314 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3520

Human Homologs

References

Uncovering the structure and function of Pseudomonas aeruginosa periplasmic proteins by an in silico approach.
Caprari S, Brandi V, Pasquadibisceglie A, Polticelli F
J Biomol Struct Dyn 2020 Sep;38(15):4508-4520
PubMed ID: 31631799
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994