Pseudomonas aeruginosa PAO1, PA3574 (nalD)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3574
Name
nalD
Replicon chromosome
Genomic location 4006510 - 4007148 (+ strand)
Transposon Mutants 6 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252264.1
GI 15598770
Affymetrix PA3574_at
Entrez 880183
GenBank AAG06962.1
INSDC AAG06962.1
NCBI Locus Tag PA3574
protein_id(GenBank) gb|AAG06962.1|AE004778_6|gnl|PseudoCAP|PA3574
TIGR NTL03PA03574
UniParc UPI00000C5A74
UniProtKB Acc Q9HY46
UniProtKB ID Q9HY46_PSEAE
UniRef100 UniRef100_W0YTN0
UniRef50 UniRef50_B7VAK7
UniRef90 UniRef90_B7VAK7

Product

Feature Type CDS
Coding Frame 1
Product Name
NalD
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -2.70
Kyte-Doolittle Hydrophobicity Value -0.400
Molecular Weight (kDa) 24.5
Isoelectric Point (pI) 6.25

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 1
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff Bit Score Percent Identity CARD Version
nalD NalD is a repressor of MexAB-OprM. Mutations lead to multidrug resistance and MexAB-OprM overexpression. [PMID:15855496] ARO:3000819 protein overexpression model Strict 429.5 100.0 3.2.5

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5H9T TRANSCRIPTION REGULATOR 12/29/15 Crystal structure of native NalD at resolution of 2.9, the secondary repressor of MexAB-OprM multidrug efflux pump in Pseudomonas aeruginosa Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) 2.89 X-RAY DIFFRACTION 100.0
5DAJ TRANSCRIPTION REGULATOR 08/20/15 Crystal structure of NalD, the secondary repressor of MexAB-OprM multidrug efflux pump in Pseudomonas aeruginosa Pseudomonas aeruginosa PAO1 2.65 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 330 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002095 (523 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3574
Search term: nalD
Search term: NalD

Human Homologs

References

Molecular cloning and characterization of acrA and acrE genes of Escherichia coli.
Ma D, Cook DN, Alberti M, Pon NG, Nikaido H, Hearst JE
J Bacteriol 1993 Oct;175(19):6299-313
PubMed ID: 8407802
Mutations in PA3574 (nalD) lead to increased MexAB-OprM expression and multidrug resistance in laboratory and clinical isolates of Pseudomonas aeruginosa.
Sobel ML, Hocquet D, Cao L, Plesiat P, Poole K
Antimicrob Agents Chemother 2005 May;49(5):1782-6
PubMed ID: 15855496