Pseudomonas aeruginosa PAO1, PA3692 (lptF)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3692
Name
lptF
Replicon chromosome
Genomic location 4134591 - 4135376 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252382.1
GI 15598888
Affymetrix PA3692_at
DNASU PaCD00007618
Entrez 879104
GenBank AAG07080.1
INSDC AAG07080.1
NCBI Locus Tag PA3692
protein_id(GenBank) gb|AAG07080.1|AE004788_10|gnl|PseudoCAP|PA3692
TIGR NTL03PA03692
UniParc UPI00000C5AC1
UniProtKB Acc Q9HXU8
UniProtKB ID Q9HXU8_PSEAE
UniRef100 UniRef100_W0YWG8
UniRef50 UniRef50_Q88MR7
UniRef90 UniRef90_W0YWG8

Product

Feature Type CDS
Coding Frame 1
Product Name
Lipotoxon F, LptF
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Detected using LC-MS/MS (28.51 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 5.97
Kyte-Doolittle Hydrophobicity Value -0.574
Molecular Weight (kDa) 28.5
Isoelectric Point (pI) 9.90

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Outer Membrane Class 3
Outer Membrane Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 456 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001985 (526 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3692
Search term: lptF
Search term: Lipotoxon F, LptF

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Conservation of the gene for outer membrane protein OprF in the family Pseudomonadaceae: sequence of the Pseudomonas syringae oprF gene.
Ullstrom CA, Siehnel R, Woodruff W, Steinbach S, Hancock RE
J Bacteriol 1991 Jan;173(2):768-75
PubMed ID: 1898935
Lipotoxin F of Pseudomonas aeruginosa is an AlgU-dependent and alginate-independent outer membrane protein involved in resistance to oxidative stress and adhesion to A549 human lung epithelia.
Damron FH, Napper J, Teter MA, Yu HD
Microbiology (Reading) 2009 Apr;155(Pt 4):1028-1038
PubMed ID: 19332805
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344