Pseudomonas aeruginosa PAO1, PA3724 (lasB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA3724
Name
lasB
Replicon chromosome
Genomic location 4168987 - 4170483 (- strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_252413.1
GI 15598919
Affymetrix PA3724_lasB_at
Entrez 880368
GenBank AAG07111.1
NCBI Locus Tag PA3724
protein_id(GenBank) gb|AAG07111.1|AE004791_8|gnl|PseudoCAP|PA3724
TIGR NTL03PA03724
UniProtKB Acc P14756
UniProtKB ID ELAS_PSEAE

Product

Feature Type CDS
Coding Frame 1
Product Name
elastase LasB
Product Name Confidence: Class 1
Synonyms neutral metalloproteinase
pseudolysin precursor
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -1.48
Kyte-Doolittle Hydrophobicity Value -0.271
Molecular Weight (kDa) 53.7
Isoelectric Point (pI) 6.74

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 1
Extracellular Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4K89 HYDROLASE 04/18/13 Crystal structure of Pseudomonas aeruginosa strain K solvent tolerant elastase PSEUDOMONAS AERUGINOSA 1.39 X-RAY DIFFRACTION 99.7
1EZM HYDROLASE 01/13/92 THREE-DIMENSIONAL STRUCTURE OF THE ELASTASE OF PSEUDOMONAS AERUGINOSA AT 1.5 ANGSTROMS RESOLUTION PSEUDOMONAS AERUGINOSA 1.5 X-RAY DIFFRACTION 100.0
3DBK HYDROLASE 06/01/08 Pseudomonas aeruginosa elastase with phosphoramidon PSEUDOMONAS AERUGINOSA 1.4 X-RAY DIFFRACTION 100.0
6F8B HYDROLASE 12/12/17 LasB bound to thiol based inhibitor PSEUDOMONAS AERUGINOSA 1.3 X-RAY DIFFRACTION 100.0
6FZX HYDROLASE 03/15/18 LasB, hydroxymate Inhibitor Complex PSEUDOMONAS AERUGINOSA 2.1 X-RAY DIFFRACTION 100.0
1U4G HYDROLASE 07/25/04 Elastase of Pseudomonas aeruginosa with an inhibitor PSEUDOMONAS AERUGINOSA 1.4 X-RAY DIFFRACTION 100.0

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
N-(1-Carboxy-3-Phenylpropyl)Phenylalanyl-Alpha-Asparagine DB02307 DrugBank 5.1.4 P14756 Elastase 100.0 498 6.5e-289

Virulence Evidence

Source 1
Database VFDB : VF0087
Category Elastase
Evidence
Host Organism
Notes
Reference
Source 2
Database PseudoCAP
Category Elastase
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Cavia porcellus
Notes
Infection model Guinea pig acute lung infection
Reference 6401692
Source 3
Database PseudoCAP
Category Elastase
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Oryctolagus cuniculus
Notes LasB mutation reduced invasion of rabbit corneal epithelial (RCE) cells by 70%
Reference 12904569

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 114 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001959 (313 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3724
Search term: lasB
Search term: elastase LasB

Human Homologs

References

Characterization and purification of an outer membrane metalloproteinase from Pseudomonas aeruginosa with fibrinogenolytic activity.
Fricke B, Parchmann O, Kruse K, Rücknagel P, Schierhorn A, Menge S
Biochim Biophys Acta 1999 Aug 30;1454(3):236-50
PubMed ID: 10452958
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Molecular characterization and nucleotide sequence of the Pseudomonas aeruginosa elastase structural gene.
Bever RA, Iglewski BH
J Bacteriol 1988 Sep;170(9):4309-14
PubMed ID: 2842313
Three-dimensional structure of the elastase of Pseudomonas aeruginosa at 1.5-A resolution.
Thayer MM, Flaherty KM, McKay DB
J Biol Chem 1991 Feb 15;266(5):2864-71
PubMed ID: 1899664
Efficient production and processing of elastase and LasA by Pseudomonas aeruginosa require zinc and calcium ions.
Olson JC, Ohman DE
J Bacteriol 1992 Jun;174(12):4140-7
PubMed ID: 1597429
Secretion of elastinolytic enzymes and their propeptides by Pseudomonas aeruginosa.
Braun P, de Groot A, Bitter W, Tommassen J
J Bacteriol 1998 Jul;180(13):3467-9
PubMed ID: 9642203