Pseudomonas aeruginosa PAO1, PA3805 (pilF)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3805
Name
pilF
Replicon chromosome
Genomic location 4264530 - 4265288 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_252494.1
GI 15599000
Affymetrix PA3805_pilF_at
Entrez 879170
GenBank AAG07192.1
INSDC AAG07192.1
NCBI Locus Tag PA3805
protein_id(GenBank) gb|AAG07192.1|AE004798_15|gnl|PseudoCAP|PA3805
TIGR NTL03PA03805
UniParc UPI00000C5B1F
UniProtKB Acc Q9HXJ2
UniProtKB ID Q9HXJ2_PSEAE
UniRef100 UniRef100_Q9HXJ2
UniRef50 UniRef50_Q9HXJ2
UniRef90 UniRef90_Q9HXJ2

Product

Feature Type CDS
Coding Frame 1
Product Name
type 4 fimbrial biogenesis protein PilF
Product Name Confidence: Class 1
Synonyms pilin biosynthetic gene PilF
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Detected using LC-MS/MS (28.54 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 0.21
Kyte-Doolittle Hydrophobicity Value -0.544
Molecular Weight (kDa) 28.5
Isoelectric Point (pI) 7.33

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Class 1
Outer Membrane Vesicle Class 1
Outer Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
2FI7 PROTEIN BINDING 12/28/05 Crystal Structure of PilF : Functional implication in the type 4 pilus biogenesis in Pseudomonas aeruginosa Pseudomonas aeruginosa 2.2 X-RAY DIFFRACTION 100.0
2HO1 PROTEIN BINDING 07/13/06 Functional Characterization of Pseudomonas Aeruginosa pilF Pseudomonas aeruginosa 2 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database VFDB : VF0082
Category Type IV pili biosynthesis
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 204 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001861 (534 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3805
Search term: pilF

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Identification of a gene, pilF, required for type 4 fimbrial biogenesis and twitching motility in Pseudomonas aeruginosa.
Watson AA, Alm RA, Mattick JS
Gene 1996 Nov 21;180(1-2):49-56
PubMed ID: 8973346
PilF is an outer membrane lipoprotein required for multimerization and localization of the Pseudomonas aeruginosa Type IV pilus secretin.
Koo J, Tammam S, Ku SY, Sampaleanu LM, Burrows LL, Howell PL
J Bacteriol 2008 Nov;190(21):6961-9
PubMed ID: 18776008
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344