Pseudomonas aeruginosa PAO1, PA3920 (copA1 )

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA3920
Name
copA1
Synonym: yvgX
Synonym: cueA
Replicon chromosome
Genomic location 4389231 - 4391609 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentPA3920 was originally identified as cueA in 2005 where its role in Cu resistance/tolerance was confirmed (PMID:16128398). Referred to as cueA in 2010 where role in Cu tolerance was also confirmed (PMID: 20233934).
Comment PA3920 later referred to as CopA1 i in order to align with naming and homologies (PMID:28760827, PMID:21091508).

Cross-References

RefSeq NP_252609.1
GI 15599115
Affymetrix PA3920_at
Entrez 879022
GenBank AAG07307.1
INSDC AAG07307.1
NCBI Locus Tag PA3920
protein_id(GenBank) gb|AAG07307.1|AE004809_7|gnl|PseudoCAP|PA3920
TIGR NTL03PA03920
UniParc UPI00000C5B78
UniProtKB Acc Q9HX93
UniProtKB ID Q9HX93_PSEAE
UniRef100 UniRef100_S0J2Y1
UniRef50 UniRef50_A0A022P3W0
UniRef90 UniRef90_A0A022P3W0

Product

Feature Type CDS
Coding Frame 1
Product Name
CopA1
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -1.04
Kyte-Doolittle Hydrophobicity Value 0.255
Molecular Weight (kDa) 83.4
Isoelectric Point (pI) 6.85

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 643 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001754 (551 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3920
Search term: copA1
Search term: CopA1

Human Homologs

Ensembl 97, assembly GRCh38.p12
ATPase copper transporting beta [Source:HGNC Symbol;Acc:HGNC:870]
E-value: 4.3e-115
Percent Identity: 36.9
Ensembl 97, assembly GRCh38.p12
ATPase copper transporting beta [Source:HGNC Symbol;Acc:HGNC:870]
E-value: 4.3e-115
Percent Identity: 36.9

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Mutations in the cueA gene encoding a copper homeostasis P-type ATPase reduce the pathogenicity of Pseudomonas aeruginosa in mice.
Schwan WR, Warrener P, Keunz E, Stover CK, Folger KR
Int J Med Microbiol 2005 Aug;295(4):237-42
PubMed ID: 16128398
Quorum-sensing regulation of a copper toxicity system in Pseudomonas aeruginosa.
Thaden JT, Lory S, Gardner TS
J. Bacteriol. 2010 May;192(10):2557-68
PubMed ID: 20233934
Copper homeostasis networks in the bacterium <i>Pseudomonas aeruginosa</i>.
Quintana J, Novoa-Aponte L, Argüello JM
J Biol Chem 2017 Sep 22;292(38):15691-15704
PubMed ID: 28760827
Distinct functional roles of homologous Cu+ efflux ATPases in Pseudomonas aeruginosa.
González-Guerrero M, Raimunda D, Cheng X, Argüello JM
Mol Microbiol 2010 Dec;78(5):1246-58
PubMed ID: 21091508