Pseudomonas aeruginosa PAO1, PA4019 (ubiX)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4019
Name
ubiX
Replicon chromosome
Genomic location 4497850 - 4498479 (- strand)

Cross-References

RefSeq NP_252708.1
GI 15599214
Affymetrix PA4019_at
DNASU PaCD00007756
Entrez 878992
GenBank AAG07406.1
INSDC AAG07406.1
NCBI Locus Tag PA4019
protein_id(GenBank) gb|AAG07406.1|AE004818_12|gnl|PseudoCAP|PA4019
TIGR NTL03PA04019
UniParc UPI0000131194
UniProtKB Acc Q9HX08
UniProtKB ID PAAD_PSEAE
UniRef100 UniRef100_Q9HX08
UniRef50 UniRef50_Q9HX08
UniRef90 UniRef90_Q9HX08

Product

Feature Type CDS
Coding Frame 1
Product Name
UbiX
Product Name Confidence: Class 1
Synonyms probable aromatic acid decarboxylase
Evidence for Translation
Charge (pH 7) -6.20
Kyte-Doolittle Hydrophobicity Value 0.153
Molecular Weight (kDa) 22.4
Isoelectric Point (pI) 4.81

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4ZAV LYASE 04/14/15 UbiX in complex with a covalent adduct between dimethylallyl monophosphate and reduced FMN Pseudomonas aeruginosa 1.4 X-RAY DIFFRACTION 100.0
4ZAG LYASE 04/13/15 Structure of UbiX E49Q mutant in complex with oxidised FMN and dimethylallyl monophosphate Pseudomonas aeruginosa 1.68 X-RAY DIFFRACTION 99.5
3ZQU LYASE 06/11/11 STRUCTURE OF A PROBABLE AROMATIC ACID DECARBOXYLASE PSEUDOMONAS AERUGINOSA 1.5 X-RAY DIFFRACTION 100.0
4ZAX TRANSFERASE 04/14/15 Structure of UbiX in complex with oxidised prenylated FMN (radical) Pseudomonas aeruginosa 1.61 X-RAY DIFFRACTION 100.0
6QLK TRANSFERASE 02/01/19 Crystal structure of F181H UbiX in complex with prFMN Pseudomonas aeruginosa 1.78 X-RAY DIFFRACTION 99.5
4ZAZ LYASE 04/14/15 Structure of UbiX Y169F in complex with a covalent adduct formed between reduced FMN and dimethylallyl monophosphate Pseudomonas aeruginosa 1.45 X-RAY DIFFRACTION 99.5
6QLJ TRANSFERASE 02/01/19 Crystal structure of F181Q UbiX in complex with an oxidised N5-C1' adduct derived from DMAP Pseudomonas aeruginosa 1.77 X-RAY DIFFRACTION 99.5
4ZAF LYASE 04/13/15 Structure of UbiX in complex with oxidised FMN and dimethylallyl monophosphate Pseudomonas aeruginosa 1.71 X-RAY DIFFRACTION 100.0
6QLH TRANSFERASE 02/01/19 Crystal structure of UbiX in complex with reduced FMN and isopentyl monophosphate Pseudomonas aeruginosa 1.57 X-RAY DIFFRACTION 100.0
6QLL TRANSFERASE 02/01/19 Crystal structure of F181H UbiX in complex with FMN and dimethylallyl monophosphate Pseudomonas aeruginosa 1.56 X-RAY DIFFRACTION 99.5
6QLV TRANSFERASE 02/01/19 Crystal structure of W200H UbiX in complex with a geranyl-FMN N5 adduct Pseudomonas aeruginosa 2.391 X-RAY DIFFRACTION 99.5
4ZAW LYASE 04/14/15 Structure of UbiX in complex with reduced prenylated FMN Pseudomonas aeruginosa 1.89 X-RAY DIFFRACTION 100.0
4ZAY LYASE 04/14/15 Structure of UbiX E49Q in complex with a covalent adduct between dimethylallyl monophosphate and reduced FMN Pseudomonas aeruginosa 1.54 X-RAY DIFFRACTION 99.5
4ZAL LYASE 04/13/15 Structure of UbiX E49Q mutant in complex with reduced FMN and dimethylallyl monophosphate Pseudomonas aeruginosa 1.62 X-RAY DIFFRACTION 99.5
4ZAN LYASE 04/13/15 Structure of UbiX Y169F in complex with oxidised FMN and dimethylallyl monophosphate Pseudomonas aeruginosa 1.76 X-RAY DIFFRACTION 99.5
6QLI TRANSFERASE 02/01/19 Crystal structure of F181Q UbiX in complex with FMN and dimethylallyl monophosphate Pseudomonas aeruginosa 1.99 X-RAY DIFFRACTION 99.5

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 371 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001664 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4019
Search term: ubiX
Search term: UbiX

Human Homologs

References

Structure of PA4019, a putative aromatic acid decarboxylase from Pseudomonas aeruginosa.
Kopec J, Schnell R, Schneider G
Acta Crystallogr Sect F Struct Biol Cryst Commun 2011 Oct 1;67(Pt 10):1184-8
PubMed ID: 22102023
UbiX is a flavin prenyltransferase required for bacterial ubiquinone biosynthesis.
White MD, Payne KA, Fisher K, Marshall SA, Parker D, Rattray NJ, Trivedi DK, Goodacre R, Rigby SE, Scrutton NS, Hay S, Leys D
Nature 2015 Jun 25;522(7557):502-6
PubMed ID: 26083743