Pseudomonas aeruginosa PAO1, PA4235 (ftnA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4235
Name
ftnA
Replicon chromosome
Genomic location 4751665 - 4752129 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252925.1
GI 15599431
Affymetrix PA4235_bfrA_at
Entrez 881830
GenBank AAG07623.1
INSDC AAG07623.1
NCBI Locus Tag PA4235
protein_id(GenBank) gb|AAG07623.1|AE004841_1|gnl|PseudoCAP|PA4235
TIGR NTL03PA04236
UniParc UPI00000C5C7E
UniProtKB Acc Q9HWF9
UniProtKB ID BFR_PSEAE
UniRef100 UniRef100_Q9HWF9
UniRef50 UniRef50_Q9HWF9
UniRef90 UniRef90_Q9HWF9

Product

Feature Type CDS
Coding Frame 1
Product Name
bacterial ferritin
Product Name Confidence: Class 1
Synonyms FtnA
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (17.94 kDa, PMID:21751344)
Charge (pH 7) -10.11
Kyte-Doolittle Hydrophobicity Value -0.504
Molecular Weight (kDa) 17.9
Isoelectric Point (pI) 4.79

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3R2S METAL BINDING PROTEIN 03/14/11 2.1A resolution structure of Doubly Soaked FtnA from Pseudomonas aeruginosa (pH 6.0) Pseudomonas aeruginosa 2.1 X-RAY DIFFRACTION 100.0
3R2M METAL BINDING PROTEIN 03/14/11 1.8A resolution structure of Doubly Soaked FtnA from Pseudomonas aeruginosa (pH 7.5) Pseudomonas aeruginosa 1.8 X-RAY DIFFRACTION 100.0
3R2R METAL BINDING PROTEIN 03/14/11 1.65A resolution structure of Iron Soaked FtnA from Pseudomonas aeruginosa (pH 6.0) Pseudomonas aeruginosa 1.65 X-RAY DIFFRACTION 100.0
3R2H METAL BINDING PROTEIN 03/14/11 1.7 A resolution structure of As-Isolated FtnA from Pseudomonas aeruginosa (pH 10.5) Pseudomonas aeruginosa 1.7 X-RAY DIFFRACTION 100.0
3R2K METAL BINDING PROTEIN 03/14/11 1.55A resolution structure of As-Isolated FtnA from Pseudomonas aeruginosa (pH 7.5) Pseudomonas aeruginosa 1.55 X-RAY DIFFRACTION 100.0
3R2L METAL BINDING PROTEIN 03/14/11 1.85A resolution structure of Iron Soaked FtnA from Pseudomonas aeruginosa (pH 7.5) Pseudomonas aeruginosa 1.85 X-RAY DIFFRACTION 100.0
3R2O METAL BINDING PROTEIN 03/14/11 1.95 A resolution structure of As-Isolated FtnA from Pseudomonas aeruginosa (pH 6.0) Pseudomonas aeruginosa 1.95 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 220 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001475 (534 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4235
Search term: ftnA

Human Homologs

References

Purification, characterization and function of bacterioferritin from the cyanobacterium Synechocystis P.C.C. 6803.
Laulhère JP, Labouré AM, Van Wuytswinkel O, Gagnon J, Briat JF
Biochem J 1992 Feb 1;281 ( Pt 3)(Pt 3):785-93
PubMed ID: 1536655
Bacterioferritin A modulates catalase A (KatA) activity and resistance to hydrogen peroxide in Pseudomonas aeruginosa.
Ma JF, Ochsner UA, Klotz MG, Nanayakkara VK, Howell ML, Johnson Z, Posey JE, Vasil ML, Monaco JJ, Hassett DJ
J Bacteriol 1999 Jun;181(12):3730-42
PubMed ID: 10368148
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
Two distinct ferritin-like molecules in Pseudomonas aeruginosa: the product of the bfrA gene is a bacterial ferritin (FtnA) and not a bacterioferritin (Bfr).
Yao H, Jepkorir G, Lovell S, Nama PV, Weeratunga S, Battaile KP, Rivera M
Biochemistry 2011 Jun 14;50(23):5236-48
PubMed ID: 21574546