Pseudomonas aeruginosa PAO1, PA4412 (murG)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4412
Name
murG
Replicon chromosome
Genomic location 4945068 - 4946141 (- strand)

Cross-References

RefSeq NP_253102.1
GI 15599608
Affymetrix PA4412_murG_at
DNASU PaCD00007912
Entrez 881264
GenBank AAG07800.1
INSDC AAG07800.1
NCBI Locus Tag PA4412
protein_id(GenBank) gb|AAG07800.1|AE004856_11|gnl|PseudoCAP|PA4412
TIGR NTL03PA04413
UniParc UPI000012F9DD
UniProtKB Acc Q9HW01
UniProtKB ID MURG_PSEAE
UniRef100 UniRef100_B7UZJ0
UniRef50 UniRef50_Q87WY5
UniRef90 UniRef90_B7UZJ0

Product

Feature Type CDS
Coding Frame 1
Product Name
UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 11.25
Kyte-Doolittle Hydrophobicity Value 0.138
Molecular Weight (kDa) 37.8
Isoelectric Point (pI) 9.99

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 2
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3S2U TRANSFERASE 05/17/11 Crystal structure of the Pseudomonas aeruginosa MurG:UDP-GlcNAc substrate complex Pseudomonas aeruginosa 2.23 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 571 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004002 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4412
Search term: murG

Human Homologs

References

Nucleotide sequence of the cell-envelope murG gene of Escherichia coli.
Mengin-Lecreulx D, Texier L, van Heijenoort J
Nucleic Acids Res 1990 May 11;18(9):2810
PubMed ID: 2187180
Nucleotide sequence involving murG and murC in the mra gene cluster region of Escherichia coli.
Ikeda M, Wachi M, Jung HK, Ishino F, Matsuhashi M
Nucleic Acids Res 1990 Jul 11;18(13):4014
PubMed ID: 2197603
The murG gene of Escherichia coli codes for the UDP-N-acetylglucosamine: N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase involved in the membrane steps of peptidoglycan synthesis.
Mengin-Lecreulx D, Texier L, Rousseau M, van Heijenoort J
J Bacteriol 1991 Aug;173(15):4625-36
PubMed ID: 1649817
The final step of peptidoglycan subunit assembly in Escherichia coli occurs in the cytoplasm.
Bupp K, van Heijenoort J
J Bacteriol 1993 Mar;175(6):1841-3
PubMed ID: 8449890