Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA4416
|
Name |
murF
|
Replicon | chromosome |
Genomic location | 4949772 - 4951148 (- strand) |
RefSeq | NP_253106.1 |
GI | 15599612 |
Affymetrix | PA4416_murF_at |
Entrez | 881289 |
GenBank | AAG07804.1 |
INSDC | AAG07804.1 |
NCBI Locus Tag | PA4416 |
protein_id(GenBank) | gb|AAG07804.1|AE004856_15|gnl|PseudoCAP|PA4416 |
TIGR | NTL03PA04417 |
UniParc | UPI00000C5D04 |
UniProtKB Acc | Q9HVZ7 |
UniProtKB ID | Q9HVZ7_PSEAE |
UniRef100 | UniRef100_W0YYS3 |
UniRef50 | UniRef50_Q87WY1 |
UniRef90 | UniRef90_Q02H24 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase
|
Synonyms |
D-ala-D-ala-adding enzyme |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | -2.64 |
Kyte-Doolittle Hydrophobicity Value | 0.162 |
Molecular Weight (kDa) | 47.4 |
Isoelectric Point (pI) | 6.52 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
4CVL | LIGASE | 03/28/14 | PaMurF in complex with AMP-PNP | PSEUDOMONAS AERUGINOSA PAO1 | 2.98 | X-RAY DIFFRACTION | 99.5 |
4CVM | LIGASE | 03/28/14 | PaMurF in complex with AMP-PNP and UDP-MurNAc-tripeptide (mDAP) | PSEUDOMONAS AERUGINOSA PAO1 | 2.06 | X-RAY DIFFRACTION | 99.5 |
4CVK | LIGASE | 03/28/14 | PaMurF in complex with UDP-MurNAc-tripeptide (mDAP) | Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT | 1.92 | X-RAY DIFFRACTION | 99.5 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 577 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG004006 (534 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA4416
Search term: murF
|
Nucleotide sequence of the murF gene encoding the UDP-MurNAc-pentapeptide synthetase of Escherichia coli.
Parquet C, Flouret B, Mengin-Lecreulx D, van Heijenoort J
Nucleic Acids Res 1989 Jul 11;17(13):5379
PubMed ID: 2668880
|
Purification and characterization of the D-alanyl-D-alanine-adding enzyme from Escherichia coli.
Duncan K, van Heijenoort J, Walsh CT
Biochemistry 1990 Mar 6;29(9):2379-86
PubMed ID: 2186811
|
Kinetic mechanism of the Escherichia coli UDPMurNAc-tripeptide D-alanyl-D-alanine-adding enzyme: use of a glutathione S-transferase fusion.
Anderson MS, Eveland SS, Onishi HR, Pompliano DL
Biochemistry 1996 Dec 17;35(50):16264-9
PubMed ID: 8973200
|
Conditionally lethal Escherichia coli murein mutants contain point defects that map to regions conserved among murein and folyl poly-gamma-glutamate ligases: identification of a ligase superfamily.
Eveland SS, Pompliano DL, Anderson MS
Biochemistry 1997 May 20;36(20):6223-9
PubMed ID: 9166795
|