Pseudomonas aeruginosa PAO1, PA4417 (murE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4417
Name
murE
Replicon chromosome
Genomic location 4951141 - 4952604 (- strand)

Cross-References

RefSeq NP_253107.1
GI 15599613
Affymetrix PA4417_murE_at
Entrez 881246
GenBank AAG07805.1
INSDC CAA58873.1
NCBI Locus Tag PA4417
protein_id(GenBank) gb|AAG07805.1|AE004856_16|gnl|PseudoCAP|PA4417
TIGR NTL03PA04418
UniParc UPI000012F9A5
UniProtKB Acc Q59650
UniProtKB ID MURE_PSEAE
UniRef100 UniRef100_Q59650
UniRef50 UniRef50_Q59650
UniRef90 UniRef90_Q59650

Product

Feature Type CDS
Coding Frame 1
Product Name
UDP-N-acetylmuramoylalanyl-D-glutamate-2, 6-diaminopimelate ligase
Product Name Confidence: Class 2
Synonyms UDP-MurNac-tripeptide synthetase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -13.24
Kyte-Doolittle Hydrophobicity Value 0.044
Molecular Weight (kDa) 51.3
Isoelectric Point (pI) 5.11

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 581 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004007 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4417
Search term: murE

Human Homologs

References

Revised interpretation of the sequence containing the murE gene encoding the UDP-N-acetylmuramyl-tripeptide synthetase of Escherichia coli.
Michaud C, Parquet C, Flouret B, Blanot D, van Heijenoort J
Biochem J 1990 Jul 1;269(1):277-8
PubMed ID: 2198024
Over-production, purification and properties of the uridine-diphosphate-N-acetylmuramoyl-L-alanyl-D-glutamate: meso-2,6-diaminopimelate ligase from Escherichia coli.
Michaud C, Mengin-Lecreulx D, van Heijenoort J, Blanot D
Eur J Biochem 1990 Dec 27;194(3):853-61
PubMed ID: 2269304
Cloning and characterization of the Pseudomonas aeruginosa pbpB gene encoding penicillin-binding protein 3.
Liao X, Hancock RE
Antimicrob Agents Chemother 1995 Aug;39(8):1871-4
PubMed ID: 7486937