Pseudomonas aeruginosa PAO1, PA4439 (trpS)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4439
Name
trpS
Replicon chromosome
Genomic location 4971986 - 4973332 (- strand)
Transposon Mutants 1 transposon mutants in PAO1

Cross-References

RefSeq NP_253129.1
GI 15599635
Affymetrix PA4439_trpS_at
Entrez 880958
GenBank AAG07827.1
INSDC AAG07827.1
NCBI Locus Tag PA4439
protein_id(GenBank) gb|AAG07827.1|AE004858_5|gnl|PseudoCAP|PA4439
TIGR NTL03PA04440
UniParc UPI0000136738
UniProtKB Acc Q9HVX6
UniProtKB ID SYW_PSEAE
UniRef100 UniRef100_Q9HVX6
UniRef50 UniRef50_Q9HVX6
UniRef90 UniRef90_Q9HVX6

Product

Feature Type CDS
Coding Frame 1
Product Name
tryptophanyl-tRNA synthetase
Product Name Confidence: Class 2
Synonyms tryptophan-tRNA ligase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -5.35
Kyte-Doolittle Hydrophobicity Value -0.199
Molecular Weight (kDa) 49.0
Isoelectric Point (pI) 5.77

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 602 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004028 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4439
Search term: trpS

Human Homologs

Ensembl 110, assembly GRCh38.p14
tryptophanyl tRNA synthetase 2, mitochondrial [Source:HGNC Symbol;Acc:HGNC:12730]
E-value: 2.0e-36
Percent Identity: 30.0

References

Tryptophanyl-tRNA synthetase from Bacillus subtilis. Characterization and role of hydrophobicity in substrate recognition.
Xu ZJ, Love ML, Ma LY, Blum M, Bronskill PM, Bernstein J, Grey AA, Hofmann T, Camerman N, Wong JT
J Biol Chem 1989 Mar 15;264(8):4304-11
PubMed ID: 2494170
Cloning and nucleotide sequence of the structural gene coding for Bacillus subtilis tryptophanyl-tRNA synthetase.
Chow KC, Wong JT
Gene 1988 Dec 20;73(2):537-43
PubMed ID: 3149612
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994