Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA4450
|
Name |
murA
|
Replicon | chromosome |
Genomic location | 4984205 - 4985470 (- strand) |
Transposon Mutants | 1 transposon mutants in PAO1 |
RefSeq | NP_253140.1 |
GI | 15599646 |
Affymetrix | PA4450_murA_at |
Entrez | 880969 |
GenBank | AAG07838.1 |
INSDC | AAG07838.1 |
NCBI Locus Tag | PA4450 |
protein_id(GenBank) | gb|AAG07838.1|AE004859_7|gnl|PseudoCAP|PA4450 |
TIGR | NTL03PA04451 |
UniParc | UPI000012F91D |
UniProtKB Acc | Q9HVW7 |
UniProtKB ID | MURA_PSEAE |
UniRef100 | UniRef100_B7UZY9 |
UniRef50 | UniRef50_P70965 |
UniRef90 | UniRef90_B7UZY9 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
UDP-N-acetylglucosamine 1-carboxyvinyltransferase
|
Synonyms |
enoylpyruvate transferase |
Evidence for Translation |
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | -6.42 |
Kyte-Doolittle Hydrophobicity Value | 0.152 |
Molecular Weight (kDa) | 44.6 |
Isoelectric Point (pI) | 5.49 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
5BQ2 | TRANSFERASE | 05/28/15 | Crystal structure of UDP-N-acetylglucosamine 1-carboxyvinyltransferase (UDP-N-acetylglucosamine enolpyruvyl transferase, EPT) from Pseudomonas aeruginosa | Pseudomonas aeruginosa PAO1 | 1.7 | X-RAY DIFFRACTION | 100.0 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 580 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG004039 (535 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA4450
Search term: murA
Search term: UDP-N-acetylglucosamine 1-carboxyvinyltransferase
|
UDP-N-acetylglucosamine 1-carboxyvinyl-transferase from Acinetobacter calcoaceticus.
Ehrt S, Hillen W
FEMS Microbiol Lett 1994 Apr 1;117(2):137-42
PubMed ID: 8181716
|
Structure of UDP-N-acetylglucosamine enolpyruvyl transferase, an enzyme essential for the synthesis of bacterial peptidoglycan, complexed with substrate UDP-N-acetylglucosamine and the drug fosfomycin.
Skarzynski T, Mistry A, Wonacott A, Hutchinson SE, Kelly VA, Duncan K
Structure 1996 Dec 15;4(12):1465-74
PubMed ID: 8994972
|
Studies on the conformational changes in the bacterial cell wall biosynthetic enzyme UDP-N-acetylglucosamine enolpyruvyltransferase (MurA).
Schönbrunn E, Svergun DI, Amrhein N, Koch MH
Eur J Biochem 1998 Apr 15;253(2):406-12
PubMed ID: 9654090
|
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
|