Pseudomonas aeruginosa PAO1, PA4760 (dnaJ)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4760
Name
dnaJ
Replicon chromosome
Genomic location 5345949 - 5347082 (- strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_253448.1
GI 15599954
Affymetrix PA4760_dnaJ_at
Entrez 881760
GenBank AAG08146.1
INSDC AAG08146.1
NCBI Locus Tag PA4760
protein_id(GenBank) gb|AAG08146.1|AE004889_9|gnl|PseudoCAP|PA4760
TIGR NTL03PA04761
UniParc UPI0000129592
UniProtKB Acc Q9HV44
UniProtKB ID DNAJ_PSEAE
UniRef100 UniRef100_B7V1H2
UniRef50 UniRef50_Q05646
UniRef90 UniRef90_A6VCL7

Product

Feature Type CDS
Coding Frame 1
Product Name
DnaJ protein
Product Name Confidence: Class 2
Synonyms heat shock protein DnaJ
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 1.39
Kyte-Doolittle Hydrophobicity Value -0.466
Molecular Weight (kDa) 40.3
Isoelectric Point (pI) 7.39

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 635 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004404 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4760
Search term: dnaJ
Search term: DnaJ protein

Human Homologs

Ensembl 110, assembly GRCh38.p14
DnaJ heat shock protein family (Hsp40) member A2 [Source:HGNC Symbol;Acc:HGNC:14884]
E-value: 3.7e-31
Percent Identity: 34.2

References

The nucleotide sequence of the Escherichia coli K12 dnaJ+ gene. A gene that encodes a heat shock protein.
Bardwell JC, Tilly K, Craig E, King J, Zylicz M, Georgopoulos C
J Biol Chem 1986 Feb 5;261(4):1782-5
PubMed ID: 3003085
Escherichia coli DnaJ and GrpE heat shock proteins jointly stimulate ATPase activity of DnaK.
Liberek K, Marszalek J, Ang D, Georgopoulos C, Zylicz M
Proc Natl Acad Sci U S A 1991 Apr 1;88(7):2874-8
PubMed ID: 1826368
NMR structure of the J-domain and the Gly/Phe-rich region of the Escherichia coli DnaJ chaperone.
Pellecchia M, Szyperski T, Wall D, Georgopoulos C, Wüthrich K
J Mol Biol 1996 Jul 12;260(2):236-50
PubMed ID: 8764403