Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA4761
|
Name |
dnaK
|
Replicon | chromosome |
Genomic location | 5347198 - 5349111 (- strand) |
Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
RefSeq | NP_253449.1 |
GI | 15599955 |
Affymetrix | PA4761_dnaK_at |
Entrez | 881765 |
GenBank | AAG08147.1 |
INSDC | AAG08147.1 |
NCBI Locus Tag | PA4761 |
protein_id(GenBank) | gb|AAG08147.1|AE004889_10|gnl|PseudoCAP|PA4761 |
TIGR | NTL03PA04762 |
UniParc | UPI00001295F4 |
UniProtKB Acc | Q9HV43 |
UniProtKB ID | DNAK_PSEAE |
UniRef100 | UniRef100_Q02FR1 |
UniRef50 | UniRef50_P38646 |
UniRef90 | UniRef90_Q02FR1 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
DnaK protein
|
Synonyms |
heat shock protein DnaK heat shock protein 70, HSP70 |
Evidence for Translation |
Verified using two-dimensional gel electrophoresis, MALDI-TOF MS and peptide mass mapping(pI:4.8, molecular weight:68.4, PMID:21674802)
Detected using LC-MS/MS (68.40 kDa, PMID:21751344)
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | -29.31 |
Kyte-Doolittle Hydrophobicity Value | -0.326 |
Molecular Weight (kDa) | 68.4 |
Isoelectric Point (pI) | 4.53 |
Individual Mappings | |
Additional evidence for subcellular localization |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 638 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG004405 (536 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA4761
Search term: dnaK
Search term: DnaK protein
|
Ensembl
110, assembly
GRCh38.p14
|
heat shock protein family A (Hsp70) member 9 [Source:HGNC Symbol;Acc:HGNC:5244]
E-value:
5.0e-206
Percent Identity:
62.1
|
Purification and properties of the Escherichia coli dnaK replication protein.
Zylicz M, Georgopoulos C
J Biol Chem 1984 Jul 25;259(14):8820-5
PubMed ID: 6086613
|
DNA sequence analysis of the dnaK gene of Escherichia coli B and of two dnaK genes carrying the temperature-sensitive mutations dnaK7(Ts) and dnaK756(Ts).
Miyazaki T, Tanaka S, Fujita H, Itikawa H
J Bacteriol 1992 Jun;174(11):3715-22
PubMed ID: 1592823
|
Inhibition of DnaK autophosphorylation by heat shock proteins and polypeptide substrates.
Panagiotidis CA, Burkholder WF, Gaitanaris GA, Gragerov A, Gottesman ME, Silverstein SJ
J Biol Chem 1994 Jun 17;269(24):16643-7
PubMed ID: 8206983
|
Structural analysis of substrate binding by the molecular chaperone DnaK.
Zhu X, Zhao X, Burkholder WF, Gragerov A, Ogata CM, Gottesman ME, Hendrickson WA
Science 1996 Jun 14;272(5268):1606-14
PubMed ID: 8658133
|
NMR solution structure of the 21 kDa chaperone protein DnaK substrate binding domain: a preview of chaperone-protein interaction.
Wang H, Kurochkin AV, Pang Y, Hu W, Flynn GC, Zuiderweg ER
Biochemistry 1998 Jun 2;37(22):7929-40
PubMed ID: 9609686
|
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
|
Proteomics of the oxidative stress response induced by hydrogen peroxide and paraquat reveals a novel AhpC-like protein in Pseudomonas aeruginosa.
Hare NJ, Scott NE, Shin EH, Connolly AM, Larsen MR, Palmisano G, Cordwell SJ
Proteomics 2011 Aug;11(15):3056-69
PubMed ID: 21674802
|
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
|
The Pseudomonas aeruginosa dnaK gene is involved in bacterial translocation across the intestinal epithelial cell barrier.
Okuda J, Yamane S, Nagata S, Kunikata C, Suezawa C, Yasuda M
Microbiology (Reading) 2017 Aug;163(8):1208-1216
PubMed ID: 28758636
|